Christopher D Snow

US Patent:

20150259710, Sep 17, 2015

Filed:

Jul 30, 2012

Appl. No.:

13/561560

Inventors:

Catherine Asleson Dundon - Englewood CO, US
Kevin Roberg-Perez - Englewood CO, US
Christopher Snow - Ft. Collins CO, US
Peter Meinhold - Denver CO, US

Assignee:

Gevo, Inc. - Englewood CO

International Classification:

C12P 7/16
C12N 9/04
C12N 9/88

Abstract:

The present invention relates to recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise at least one nucleic acid molecule encoding a polypeptide with keto-isovalerate decarboxylase (KIVD) activity, wherein said polypeptide is at least about 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to a polypeptide selected from SEQ ID NOs: 1-214. Also provided are modified decarboxylases exhibiting an improved ability to utilize α-ketoisovalerate as a substrate in various beneficial enzymatic conversions.

US Patent:

20140295512, Oct 2, 2014

Filed:

Jun 15, 2012

Appl. No.:

14/126606

Inventors:

Thomas Buelter - Duisburg, DE
Doug Lies - Parker CO, US
Christopher Smith - Parker CO, US
Peter Meinhold - Topanga CA, US
Ruth Berry - Bellevne WA, US
Christopher Snow - Fort Collins CO, US
Sabine Bastian - Pasadena CA, US

Assignee:

CALIFORNIA INSTITUTE OF TECHNOLOGY - Pasadena CA
GEVO, INC. - Englewood CO

International Classification:

C12P 7/16
C12N 9/04

US Classification:

435160, 4352542, 435190

Abstract:

The present invention relates to recombinant microorganisms comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI) or modified NADH-dependent variant thereof, wherein said KARI is at least about 60% identical to SEQ ID NO: 2. In various aspects of the invention, the recombinant microorganisms may comprise an isobutanol producing metabolic pathway and can be used in methods of making isobutanol.