Christopher R Tate

US Patent:

52120678, May 18, 1993

Filed:

Mar 30, 1989

Appl. No.:

7/330479

Inventors:

Edward T. Mallinson - Columbia MD
Christopher R. Tate - Frederick MD
Russell G. Miller - Glen Dale MD

Assignee:

The United States of America as represented by the Secretary of
Agriculture - Washington DC
The University of Maryland - College Park MD

International Classification:

C12Q 110
C12Q 102
C12Q 124
C12Q 122

US Classification:

435 38

Abstract:

A swift, accurate assay for the detection of salmonella contamination in a livestock environments, comprises sampling the floor of the livestock holding area with drag swabs for about 10 or more minutes, after which said swabs are maintained in a static condition, at reduced temperature, in the presence of double strength skim milk, until ready for testing. The swabs are transferred to a salmonella-preferential growth medium, such as tetrathionate broth and subsequently assayed. To reduce non-salmonella "look alike" or masking bacterial colonies, Novobiocin may be administered to the culture media.

US Patent:

52081508, May 4, 1993

Filed:

Apr 8, 1991

Appl. No.:

7/681358

Inventors:

Christopher Tate - Frederick MD
Russell Miller - Glen Dale MD
Edward Mallinson - Columbia MD
Sammy Joseph - Derwood MD

Assignee:

The University of Maryland - College Park MD
The United States of America as represented by the Secretary of
Agriculture - Washington DC

International Classification:

C12Q 110
C12Q 104
C12Q 124
C12Q 102
C12Q 122
C12Q 106
C12Q 108
C12N 120

US Classification:

435 38

Abstract:

An improved plating media incorporates TERGITOL. RTM. 4, an alkyl sodium sulfate biological detergent, in a Xylose-lysine agar base. The plating media may also include peptone and sulfapyridine to further inhibit Citrobacter growth. The media is highly preferential for Salmonella, giving a greater number of Salmonella positives, and reduced competitive organisms. When incorporated with drag swab methodology employing skim milk or evaporated milk as a holding media, which may advantageously include novobiocin to suppress unwanted bacterial growth, coupled with a tetrathionate-culturing broth or other selective enrichment broth, the plating media completes a highly sensitive assay for the detection of Salmonella, particularly keyed for poultry and livestock handling structures, and for veterinary and human clinical laboratory applications.