Christopher R Tate

US Patent:

52081508, May 4, 1993

Filed:

Apr 8, 1991

Appl. No.:

7/681358

Inventors:

Christopher Tate - Frederick MD
Russell Miller - Glen Dale MD
Edward Mallinson - Columbia MD
Sammy Joseph - Derwood MD

Assignee:

The University of Maryland - College Park MD
The United States of America as represented by the Secretary of
Agriculture - Washington DC

International Classification:

C12Q 110
C12Q 104
C12Q 124
C12Q 102
C12Q 122
C12Q 106
C12Q 108
C12N 120

US Classification:

435 38

Abstract:

An improved plating media incorporates TERGITOL. RTM. 4, an alkyl sodium sulfate biological detergent, in a Xylose-lysine agar base. The plating media may also include peptone and sulfapyridine to further inhibit Citrobacter growth. The media is highly preferential for Salmonella, giving a greater number of Salmonella positives, and reduced competitive organisms. When incorporated with drag swab methodology employing skim milk or evaporated milk as a holding media, which may advantageously include novobiocin to suppress unwanted bacterial growth, coupled with a tetrathionate-culturing broth or other selective enrichment broth, the plating media completes a highly sensitive assay for the detection of Salmonella, particularly keyed for poultry and livestock handling structures, and for veterinary and human clinical laboratory applications.