Connie R Hansen

US Patent:

8043839, Oct 25, 2011

Filed:

Feb 14, 2007

Appl. No.:

12/279326

Inventors:

David Weiner - Del Mar CA, US
David Blum - Nashville TN, US
Alexander Varvak - San Diego CA, US
Shaun Healey - Carlsbad CA, US
Kristine Chang - San Diego CA, US
Geoff Hazlewood - Newbury, GB
Thomas Todaro - San Diego CA, US
Grace Desantis - San Diego CA, US
Hwai Chang - San Marcos CA, US
Connie Jo Hansen - San Diego CA, US
Scott W. Beaver - San Marcos CA, US
Thomas Woodward - Scottsville VA, US
Charles Hancock - San Marcos CA, US

Assignee:

Verenium Corporation - San Diego CA

International Classification:

C12N 9/24
C12N 1/20
C12N 1/15
C12N 1/16
C12N 1/19
C12N 15/70
C12P 21/06
C07H 21/04
A01H 5/00

US Classification:

435200, 435 691, 4353201, 4352523, 536 232, 800298, 800312, 800314, 800320, 8003201, 8003202, 8003203

Abstract:

The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e. g. , catalyzing hydrolysis of internal β-1,4-xylosidic linkages or endo-β-1,4-ghicanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.

US Patent:

20030228616, Dec 11, 2003

Filed:

May 12, 2003

Appl. No.:

10/435766

Inventors:

Bahram Arezi - Carlsbad CA, US
Holly Hogrefe - San Diego CA, US
Joseph Sorge - Wilson WY, US
Connie Hansen - San Diego CA, US

Assignee:

Stratagene

International Classification:

C12Q001/68
C07H021/04
C12P019/34
C12N009/22
C12N001/20
C12N015/74

US Classification:

435/006000, 435/069100, 435/199000, 435/252300, 435/320100, 435/091200, 536/023200

Abstract:

The present invention relates to compositions and kits comprising a mutant DNA polymerase with increased reverse transcriptase activity. The invention also relates to methods for using the subject compositions and kits.

US Patent:

20050123940, Jun 9, 2005

Filed:

May 26, 2004

Appl. No.:

10/853973

Inventors:

Joseph Sorge - Del Mar CA, US
Holly Hogrefe - San Diego CA, US
Connie Hansen - San Diego CA, US
Bahram Arezi - Carlsbad CA, US
Rebecca Mullinax - San Diego CA, US

International Classification:

C12Q001/68
C12P019/34
C12N009/22

US Classification:

435006000, 435091200, 435199000

Abstract:

The present invention relates to composition, kits and methods comprising a mutant DNA polymerase exhibiting increased reverse transcriptase activity. The invention also relates to methods of generating modified cDNA.

US Patent:

20080280291, Nov 13, 2008

Filed:

May 15, 2006

Appl. No.:

11/435018

Inventors:

Joseph A. Sorge - Del Mar CA, US
Connie Jo Hansen - San Diego CA, US
Holly Hogrefe - San Diego CA, US

International Classification:

C12Q 1/68
C12N 9/00

US Classification:

435 6, 435183

Abstract:

The invention features a novel isolated Family B DNA polymerase, a polymerase JDF-3, and mutant recombinant forms thereof. Mutant polymerases of the invention are deficient in 3′ to 5′ exonuclease activity and/or exhibit reduced discrimination against non-conventional nucleotides relative to the wild-type form of the polymerase.

US Patent:

56817266, Oct 28, 1997

Filed:

Sep 2, 1993

Appl. No.:

8/116049

Inventors:

William David Huse - Del Mar CA
Connie Jo Hansen - San Diego CA

Assignee:

Stratagene - La Jolla CA

International Classification:

C12P 1934

US Classification:

435 9152

Abstract:

The present invention provides an improved method for the synthesis of double stranded DNA, particularly complementary DNA for the construction of directional complementary DNA libraries. The method comprises synthesizing a first strand of DNA complementary to a selected RNA or DNA template by contacting with the template a linker/primer comprising a selected restriction site, a suitable RNA or DNA dependent DNA polymerase, and substrates comprising a deoxyribonucleotide triphosphate analog. The linker/primer and deoxyribonucleotide triphosphate analog are selected such that incorporation of the nucleotide analog in the first strand substantially protects the double stranded DNA from cleavage, under conditions sufficient to cleave or substantially cleave the linker/primer, at the selected restriction site.

US Patent:

61435317, Nov 7, 2000

Filed:

Jul 22, 1997

Appl. No.:

8/899029

Inventors:

William David Huse - Del Mar CA
Connie Jo Hansen - San Diego CA

Assignee:

Stratagene - La Jolla CA

International Classification:

C12P 1934
C07H 2104

US Classification:

435 9151

Abstract:

The present invention provides an improved method for the synthesis of double stranded DNA, particularly complementary DNA for the construction of directional complementary DNA libraries. The method comprises synthesizing a first strand of DNA complementary to a selected RNA or DNA template by contacting with the template a linker/primer comprising a selected restriction site, a suitable RNA or DNA dependent DNA polymerase, and substrates comprising a deoxyribonucleotide triphosphate analog. The linker/primer and deoxyribonucleotide triphosphate analog are selected such that incorporation of the nucleotide analog in the first strand substantially protects the double stranded DNA from cleavage, under conditions sufficient to cleave or substantially cleave the linker/primer, at the selected restriction site.

US Patent:

20170342483, Nov 30, 2017

Filed:

May 27, 2016

Appl. No.:

15/167823

Inventors:

- Santa Clara CA, US
Michael Borns - Escondido CA, US
Holly Hogrefe - San Diego CA, US
Connie Hansen - San Diego CA, US

International Classification:

C12Q 1/68

Abstract:

Provided herein, among other things, are a variety of methods for transposase-mediated tagging and amplification of short DNA fragments, e.g., between about 150 bp and 1.5 Kb in length. In some aspects, the method includes tagging the DNA fragments with a first primer sequence using barcoded transposases followed by a primer extension reaction to introduce a second primer sequence, e.g., using random or gene-specific primers. Kits for performing this method are also provided.