Connie V Ward

US Patent:

7932073, Apr 26, 2011

Filed:

Aug 8, 2007

Appl. No.:

11/891249

Inventors:

Sarah Teter - Davis CA, US
Connie Ward - Hamilton MT, US
Joel Cherry - Davis CA, US
Aubrey Jones - Davis CA, US
Paul Harris - Carnation WA, US
Jung Yi - Sacramento CA, US

Assignee:

Novozymes, Inc. - Davis CA

International Classification:

C12N 9/42
C12S 3/00
C11D 3/00

US Classification:

435209, 510382, 435267

Abstract:

The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

US Patent:

20050048619, Mar 3, 2005

Filed:

Aug 25, 2004

Appl. No.:

10/926223

Inventors:

Sarah Teter - Davis CA, US
Joel Cherry - Davis CA, US
Connie Ward - Woodland CA, US
Aubrey Jones - Davis CA, US
Paul Harris - Carnation WA, US
Jung Yi - Sacramento CA, US

Assignee:

Novozymes Biotech, Inc. - Davis CA

International Classification:

C07H021/04
C12N009/24
C12P021/04

US Classification:

435069100, 536023200, 435200000, 435320100, 435325000

Abstract:

The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

US Patent:

20060172379, Aug 3, 2006

Filed:

May 26, 2005

Appl. No.:

11/140150

Inventors:

Sarah Teter - Davis CA, US
Michael Lamsa - Davis CA, US
Joel Cherry - Davis CA, US
Connie Ward - Woodland CA, US

Assignee:

Novozymes, Inc. - Davis CA

International Classification:

C12P 21/06
C12N 1/16
C12N 15/74

US Classification:

435069100, 435254300, 435484000

Abstract:

The present invention relates to methods for expression screening of filamentous fungal transformants, comprising: (a) isolating single colony transformants of a DNA library introduced into ; (b) preparing DNA from each of the single colony transformants; (c) introducing a sample of each of the DNA preparations of step (b) into separate suspensions of protoplasts of a filamentous fungus to obtain transformants thereof, wherein each transformant contains one or more copies of an individual polynucleotide from the DNA library; (d) growing the individual filamentous fungal transformants of step (c) on selective growth medium, thereby permitting growth of the filamentous fungal transformants, while suppressing growth of untransformed filamentous fungi; and (e) measuring activity or a property of each polypeptide encoded by the individual polynucleotides. The present invention also relates to isolated polynucleotides encoding polypeptides of interest obtained by such methods, to nucleic acid constructs, expression vectors, and recombinant host cells comprising the isolated polynucleotides, and to methods of producing the polypeptides encoded by the isolated polynucleotides.