Courtney E. Maus - Pasadena MD, US Ray A. Mc Millian - Timonium MD, US
Assignee:
Becton, Dickinson and Company - Franklin Lakes NJ
International Classification:
C12Q 1/68
US Classification:
435 6
Abstract:
A region of the cytotoxin gene has been identified which is useful for performing amplification assays to determine specifically whether is present in the sample being tested. Oligonucleotides useful for performing thermal Strand Displacement Assay (tSDA) reactions on this gene are disclosed. The disclosed oligonucleotides can be used in an assay which is specific for multiple strains of and which does not show cross reactivity with the genomes of other microorganisms or with human DNA.
Assay For Chlamydia Trachomatis By Amplification And Detection Of Chlamydia Trachomatis Pmpa Gene
A region of the pmpA gene has been identified which is useful for performing amplification assays to determine specifically whether is present in the sample being tested. Oligonucleotides useful for performing thermal Strand Displacement Assay (tSDA) reactions on this gene are disclosed. The disclosed oligonucleotides can be used in an assay which is specific for multiple strains of and which does not show cross reactivity with the genomes of other microorganisms or with human DNA.