Craig James Obergfell

US Patent:

20150361481, Dec 17, 2015

Filed:

Jun 11, 2015

Appl. No.:

14/736509

Inventors:

- Carlsbad CA, US
Chieh-Yuan LI - El Cerrito CA, US
Brian REED - Woodbridge CT, US
Craig OBERGFELL - San Diego CA, US
Devin DRESSMAN - Pittsburgh PA, US
Abraham ROSENBAUM - Waterbury CT, US
Scott BENSON - Alameda CA, US
Andi BROKA - Carlsbad CA, US

International Classification:

C12Q 1/68

Abstract:

In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads.