Daniel J Cohn

US Patent:

6818428, Nov 16, 2004

Filed:

Jul 2, 2001

Appl. No.:

09/898165

Inventors:

Daniel H. Cohn - Santa Monica CA
Muhammad Faiyaz-Ul-Haque - Islamabad, PK
Lily King - Los Angeles CA
Deborah Krakow - Los Angeles CA

Assignee:

Cedars Sinai Medical Center - Los Angeles CA

International Classification:

C12N 912

US Classification:

435194, 4352523, 4353201, 536 232, 4241921

Abstract:

Disclosed are isolated polynucleotides containing nucleic acid segments encoding human or murine ATP sulfurylase/APS kinase, also known as PAPS synthetase (PAPSS), particularly PAPSS2 and Papss2 proteins. Also disclosed are nucleic acid constructs, including vectors, probes, primers, and primer pairs containing novel PAPSS2 and Papss2 gene sequences. A genetically modified vertebrate cell containing a nucleic acid construct of the present invention and a non-human vertebrate comprising the cell are also disclosed. Based on the present PAPSS2-specific polynucleotides and nucleic acid constructs, are genetic testing kits and methods for diagnosing spondyloepimetaphyseal dysplasia (SEMD) in a human subject, of identifying a human carrier of an heritable allele associated with SEMD, and of gene therapy or protein therapy for treating a human subject having an osteoarthritic disorder, which is caused or aggravated by deficient enzymatic sulfation activity. Also disclosed is a protein therapy method for treating a human subject having an osteoarthritic disorder caused or aggravated by deficient enzymatic sulfation activity that employs an inventive PAPSS2 fusion protein. Also disclosed are an isolated antibody or antibody fragment that selectively binds a PAPSS2 or Papss2 protein.

US Patent:

20030195162, Oct 16, 2003

Filed:

Jul 2, 2001

Appl. No.:

09/898200

Inventors:

Daniel Cohn - Santa Monica CA, US
Muhammad Faiyaz-Ul-Haque - Islamabad, PK
Lily King - Los Angeles CA, US
Deborah Krakow - Los Angeles CA, US

International Classification:

C12Q001/68
C07H021/04
A61K048/00
C12P019/34

US Classification:

514/044000, 435/006000, 435/091200, 536/023200, 536/024300

Abstract:

Disclosed are isolated polynucleotides containing nucleic acid segments encoding human or murine ATP sulfurylase/APS kinase, also known as PAPS synthetase (PAPSS), particularly PAPSS2 and Papss2 proteins. Also disclosed are nucleic acid constructs, including vectors, probes, primers, and primer pairs containing novel PAPSS2 and Papss2 gene sequences. A genetically modified vertebrate cell containing a nucleic acid construct of the present invention and a non-human vertebrate comprising the cell are also disclosed. Based on the present PAPSS2-specific polynucleotides and nucleic acid constructs, are genetic testing kits and methods for diagnosing spondyloepimetaphyseal dysplasia (SEMD) in a human subject, of identifying a human carrier of an heritable allele associated with SEMD, and of gene therapy or protein therapy for treating a human subject having an osteoarthritic disorder, which is caused or aggravated by deficient enzymatic sulfation activity. Also disclosed is a protein therapy method for treating a human subject having an osteoarthritic disorder caused or aggravated by deficient enzymatic sulfation activity that employs an inventive PAPSS2 fusion protein. Also disclosed are an isolated antibody or antibody fragment that selectively binds a PAPSS2 or Papss2 protein.

Address:

7701 Westlawn Ave, Los Angeles, CA 90045

Phone:

3104109088

VIN:

3GCAAADB0AS594764

Make:

CHEVROLET

Model:

HHR

Year:

2010