Daniel S Cullen

US Patent:

6379928, Apr 30, 2002

Filed:

Dec 10, 1999

Appl. No.:

09/468265

Inventors:

Randy Michael Berka - San Mateo CA
Daniel Cullen - Madison WI
Gregory Lawrence Gray - South San Francisco CA
Kirk James Hayenga - Burlingame CA
Virgil Bryan Lawlis - San Mateo CA

Assignee:

Genencor International, Inc. - Palo Alto CA

International Classification:

C12P 2100

US Classification:

435 711, 435 691, 435 697, 435 698, 435183, 435205, 435223, 435225, 43525411, 4352543, 4352544, 4352546, 4352548

Abstract:

Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence. Preferably functional polyadenylation sequences are operably linked to the 3â terminus of the DNA sequence encoding the heterologous polypeptides.

US Patent:

20030044955, Mar 6, 2003

Filed:

Mar 20, 2002

Appl. No.:

10/105481

Inventors:

Randy Berka - San Mateo CA, US
Daniel Cullen - Madison WI, US
Gregory Gray - South San Francisco CA, US
Kirk Hayenga - Burlingame CA, US
Virgil Lawlis - San Mateo CA, US

Assignee:

Genencor International, Inc.

International Classification:

C12N009/30
C07H021/04
C12N001/16
C12N001/18
C12P021/02

US Classification:

435/203000, 435/254200, 435/069100, 435/320100, 536/023200

Abstract:

Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence. Preferably functional polyadenylation sequences are operably linked to the 3′ terminus of the DNA sequence encoding the heterologous polypeptides. Each of the above described vectors are used in novel processes to transform a filamentous fungus wherein the DNA sequences encoding the signal sequence and heterologous polypeptide are expressed. The thus synthesized polypeptide is thereafter secreted from the filamentous fungus.

US Patent:

20030224482, Dec 4, 2003

Filed:

Feb 6, 2003

Appl. No.:

10/360081

Inventors:

Randy Berka - San Mateo CA, US
Daniel Cullen - Madison WI, US
Gregory Gray - South San Francisco CA, US
Kirk Hayenga - Burlingame CA, US
Virgil Lawlis - San Mateo CA, US

Assignee:

Genencor International, Inc.

International Classification:

C12P021/02
C12N001/16
C12N015/74

US Classification:

435/069100, 435/484000, 435/320100, 435/254300, 435/254800

Abstract:

Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence. Preferably functional polyadenylation sequences are operably linked to the 3′ terminus of the DNA sequence encoding the heterologous polypeptides. Each of the above described vectors are used in novel processes to transform a filamentous fungus wherein the DNA sequences encoding the signal sequence and heterologous polypeptide are expressed. The thus synthesized polypeptide is thereafter secreted from the filamentous fungus.

US Patent:

55784631, Nov 26, 1996

Filed:

Aug 2, 1994

Appl. No.:

8/284942

Inventors:

Randy M. Berka - San Mateo CA
Daniel Cullen - Madison WI
Gregory L. Gray - South San Francisco CA
Kirk J. Hayenga - Burlingame CA
Virgil B. Lawlis - San Mateo CA

Assignee:

Genencor International, Inc. - South San Francisco CA

International Classification:

C12N 115
C12N 1509
C12N 1556
C12N 1580

US Classification:

435 691

Abstract:

Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence. Preferably functional polyadenylation sequences are operably linked to the 3' terminus of the DNA sequence encoding the heterologous polypeptides.

US Patent:

53647700, Nov 15, 1994

Filed:

Sep 25, 1989

Appl. No.:

7/413010

Inventors:

Randy M. Berka - San Mateo CA
Daniel Cullen - Madison WI
Gregory L. Gray - South San Francisco CA
Kirk J. Hayenga - Burlingame CA
Virgil B. Lawlis - San Mateo CA

Assignee:

Genencor International Inc. - San Francisco CA

International Classification:

C12N 115
C12N 1509
C12N 1556
C12N 1580

US Classification:

435 691

Abstract:

Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence. Preferably functional polyadenylation sequences are operably linked to the 3' terminus of the DNA sequence encoding the heterologous polypeptides.

US Patent:

61718173, Jan 9, 2001

Filed:

Nov 24, 1997

Appl. No.:

8/976967

Inventors:

Randy Michael Berka - San Mateo CA
Daniel Cullen - Madison WI
Gregory Lawrence Gray - South San Francisco CA
Kirk James Hayenga - Burlingame CA
Virgil Bryan Lawlis - San Mateo CA

Assignee:

Genencor International, Inc. - South San Francisco CA

International Classification:

C12N 1500
C12N 119
C12P 2100
C07H 2104

US Classification:

435 691

Abstract:

Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence. Preferably functional polyadenylation sequences are operably linked to the 3' terminus of the DNA sequence encoding the heterologous polypeptides.

US Patent:

60047852, Dec 21, 1999

Filed:

Jun 7, 1995

Appl. No.:

8/484384

Inventors:

Randy Michael Berka - San Mateo CA
Daniel Cullen - Madison WI
Gregory Lawrence Gray - South San Francisco CA
Kirk James Hayenga - Burlingame CA
Virgil Bryan Lawlis - San Mateo CA

Assignee:

Genencor International Inc. - Palo Alto CA

International Classification:

C12N 115
C12N 1580

US Classification:

4351723

Abstract:

Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence. Preferably functional polyadenylation sequences are operably linked to the 3' terminus of the DNA sequence encoding the heterologous polypeptides.

US Patent:

61034908, Aug 15, 2000

Filed:

Jun 17, 1994

Appl. No.:

8/261989

Inventors:

Randy Michael Berka - San Mateo CA
Daniel Cullen - Madison WI
Gregory Lawrence Gray - South San Francisco CA
Kirk James Hayenga - Burlingame CA
Virgil Bryan Lawlis - San Mateo CA

Assignee:

Genencor International, Inc. - South San Francisco CA

International Classification:

C12N 1500
C12N 115
C12N 1563
C12N 1511

US Classification:

435 691

Abstract:

Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence. Preferably functional polyadenylation sequences are operably linked to the 3' terminus of the DNA sequence encoding the heterologous polypeptides.