Dupont Feb 2011 - Mar 2015
Research Manager and Senior Research Scientist
Dupont Feb 2005 - Feb 2011
Senior Research Microbiologist
Dupont Feb 2002 - Feb 2005
Research Microbiologist
Centers For Disease Control and Prevention Sep 1996 - Jul 1997
Emerging Infectious Disease Fellow
Eurofins Sep 1996 - Jul 1997
Associate Director of Science
Education:
University of South Florida 1997 - 2001
Doctorates, Doctor of Philosophy, Molecular Biology, Microbiology
Ohio University Heritage College of Osteopathic Medicine 1992 - 1996
Bachelors, Bachelor of Science, Microbiology, Philosophy
Skills:
Microbiology Assay Development R&D Biotechnology Pcr Molecular Biology Science Food Microbiology Life Sciences Lifesciences Qpcr Product Development Biochemistry Food Science Gel Electrophoresis Food Technology Gmp Commercialization Genetics Technology Transfer Dna Research and Development Protein Chemistry Project Management Real Time Pcr Pcr Primer Design Real Time Pcr Scorpion Chemistry Multiplex Real Time Pcr Assay Optimization and Chemistries Hplc Chromatography Infectious Diseases Polymerase Chain Reaction Six Sigma Fermentation Fda Pcr Probe Design Real Time Pcr Taq Man Chemistry Real Time Pcr Molecular Beacons Chemistry Product Commercialization Real Time Pcr Ampliflour Chemistry Design of Experiments Sample Preparation Real Time Pcr Assay Optimization Aoac Study Design Afnor Study Design Health Canada Study Design Biofilms Microbially Influenced Corrosion Molecular Subtyping Techniques Food Safety
Interests:
Writing Simpsons Seasons 1 9 Logic Modern Western Philosophy Comics Anime and Manga Quantum Physics Cosmology Golf Ancient Western Philosophy Star Wars Analytic Philosophy Philosophy of Wittgenstein See +See Less
Laura Bedzyk - Odessa DE, US Daniel DeMarco - Wilmington DE, US Richard Ebersole - Wilmington DE, US Raymond Jackson - Newark DE, US Stephen Varkey - Newark DE, US Rick Ye - Hockessin DE, US
Assignee:
E.I. du Pont de Nemours and Company - Wilmington DE
International Classification:
C12Q 1/68 C12P 19/34
US Classification:
435 6, 435 912
Abstract:
Methods for the direct detection of diagnostic target RNA have been developed, which obviate the need for time consuming RNA purification and isolation procedures.
This invention pertains to the protection of metals from corrosion and/or fouling from exposure to environments such as, for example, seawater. The protection in accordance with this invention is achieved through the use of conductive zinc-containing coatings in combination with applied current cathodic protection.
LAURA A. BEDZYK - Odessa DE, US Daniel R. Demarco - Wilmington DE, US Richard C. Ebersole - Wilmington DE, US Raymond E. Jackson - Newark DE, US Stephen Varkey - Newark DE, US Rick Ye - Hockessin DE, US
Assignee:
E. I. DU PONT DE NEMOURS AND COMPANY - Wilmington DE
International Classification:
C12Q 1/68
US Classification:
435 6
Abstract:
Methods for the direct detection of diagnostic target RNA have been developed, which obviate the need for time consuming RNA purification and isolation procedures.
Process And Device For Collecting Nucleic Acids Of Microorganisms From A Particulate Sample
Stephen Varkey - Newark DE, US Daniel R. DeMarco - Wilmington DE, US Mark A. Jensen - West Chester PA, US
International Classification:
C12Q 1/68 C07H 21/04
US Classification:
435 611, 536 2432
Abstract:
This invention relates to a rapid method for detection and characterization of STEC bacteria based on the presence of nucleic acid sequences, in particular, to a PCR-based method for detection, and to oligonucleotide molecules and reagents and kits useful therefore. This method is preferably employed to detect STEC bacteria in a food or water sample, such as a beef enrichment. The present invention further relates to isolated polynucleotides, replication compositions, kits, and reagent tablets for carrying out the method of the present invention.
Sequences And Their Use For Detection Of Salmonella
E. I. DU PONT DE NEMOURS AND COMPANY - Wilmington DE, US Daniel R. DeMarco - Wilmington DE, US
Assignee:
E. I. DU PONT DE NEMOURS AND COMPANY - Wilmington DE
International Classification:
C12Q 1/68
US Classification:
435 611, 536 2432
Abstract:
This invention relates to a rapid method for detection of in a sample based on the presence of nucleic acid sequences, in particular, to a PCR-based method for detection, and to oligonucleotide molecules and reagents and kits useful therefore. In certain embodiments, the method is employed to detect in a food or water sample. The present invention further relates to isolated polynucleotides, replication compositions, and kits for carrying out the method of the present invention.
Sequences And Their Use For Detection Of Salmonella Enteritidis And/Or Salmonella Typhimurium
- Wilmington DE, US Daniel R. Demarco - Wilmington DE, US Stephen Varkey - Newark DE, US Jun Li - Chadds Ford PA, US
International Classification:
C12Q 1/68
Abstract:
This invention relates to a rapid, accurate method for detection and characterization of and/or based on the presence of nucleic acid sequences, in particular, to a PCR-based method for detection, and to oligonucleotide molecules and reagents and kits useful therefore. This method is preferably employed to detect and/or in an environmental sample. The present invention further relates to replication compositions and kits for carrying out the method of the present invention.
Sequences And Their Use For Detection And Characterization Of Stec Bacteria
- Wilmington DE, US Daniel R. DeMarco - Wilmington DE, US Mark A. Jensen - West Chester PA, US
International Classification:
C12Q 1/68
US Classification:
536 2432
Abstract:
This invention relates to a rapid method for detection and characterization of STEC bacteria based on the presence of nucleic acid sequences, in particular, to a PCR-based method for detection, and to oligonucleotide molecules and reagents and kits useful therefore. This method is preferably employed to detect STEC bacteria in a food or water sample, such as a beef enrichment. The present invention further relates to isolated polynucleotides, replication compositions, kits, and reagent tablets for carrying out the method of the present invention.
Youtube
9. Chamber Of Reflection - Mac DeMarco (Exten...
Extended version of the music "Chamber Of Reflection" by Mac DeMarco.
Duration:
5m 6s
Ask an Expert - Daniel DeMarco
Duration:
44s
Friday Fun Fact - Daniel DeMarco
Duration:
43s
Daniel Caesar - Get You ft. Kali Uchis [Offic...
Written by Daniel Caesar & Kali Uchis Produced by Jordan Evans & Matth...
Duration:
4m 31s
MONLOGO / DANIEL DEMARCO
Gracias por los comentarios y no se olviden de los LIKEs. Suscribite p...
Frank Manfredi, Mary Flanagan, Fred Seestadt, Patrick Connolly, Armando Varone, Margaret Sestrom, Jim Murphy, Robert Barry, Joseph Molloy, Dorian Boer, Christine Carson, Denise Lucarelli
Frank Manfredi, Fred Seestadt, Patrick Connolly, Armando Varone, Margaret Sestrom, Jim Murphy, Ed Gaherty, Robert Barry, Joseph Molloy, Dorian Boer, Christine Carson