Daniel K Howe

US Patent:

7256282, Aug 14, 2007

Filed:

Jun 1, 2006

Appl. No.:

11/445045

Inventors:

Daniel K. Howe - Lexington KY, US

Assignee:

University of Kentucky Research Foundation - Lexington KY

International Classification:

C07H 21/04

US Classification:

536 237, 536 241, 536 242, 536 2432, 4353201

Abstract:

The present invention provides novel isolated nucleic acids encoding antigenic proteins derived from , or unique fragments thereof. In particular, the invention provides novel isolated nucleic acids encoding membrane-associated polypeptides SnSAG2, SnSAG3, and SnSAG4. Also provided are purified antigenic polypeptide fragments encoded by the novel nucleic acid sequences set forth herein that encode for SnSAG2, SnSAG3, and SnSAG4. Also provided are isolated nucleic acids capable of selectively hybridizing with the nucleic acid from. The invention also provides vectors comprising the nucleic acids of the invention encoding an antigenic protein derived from or a unique fragment thereof and provides the vector in a host capable of expressing the polypeptide encoded by that nucleic acid. Finally, the invention provides purified polyclonal and/or monoclonal antibodies specifically reactive with and a method of detection of utilizing the antibodies of the invention.

US Patent:

7524946, Apr 28, 2009

Filed:

Jul 9, 2007

Appl. No.:

11/774830

Inventors:

Daniel K. Howe - Lexington KY, US

Assignee:

University of Kentucky Research Foundation - Lexington KY

International Classification:

C07H 21/04

US Classification:

536 237, 4353201, 536 241, 536 242, 536 2432

Abstract:

The present invention provides novel isolated nucleic acids encoding antigenic proteins derived from or unique fragments thereof. In particular, the invention provides novel isolated nucleic acids encoding membrane-associated polypeptides SnSAG2, SnSAG3, and SnSAG 4. Also provided are purified antigenic polypeptide fragments encoded by the novel nucleic acid sequences set forth herein that encode for SnSAG2, SnSAG3, and SnSAG 4. Also provided are isolated nucleic acids capable of selectively hybridizing with the nucleic acid from The invention also provides vectors comprising the nucleic acids of the invention encoding an antigenic protein derived from or a unique fragment thereof and provides the vector in a host capable of expressing the polypeptide encoded by that nucleic acid. Finally, the invention provides purified polyclonal and/or monoclonal antibodies specifically reactive with and a method of detection of utilizing the antibodies of the invention.

US Patent:

20020146748, Oct 10, 2002

Filed:

Jan 22, 2001

Appl. No.:

09/767037

Inventors:

L. David Sibley - University City MO, US
Daniel Howe - Lexington KY, US

International Classification:

G01N033/53
G01N033/569
C07H021/04
C12N009/00
C07K016/20

US Classification:

435/007220, 435/183000, 536/023200, 530/388200

Abstract:

An immunological assay method is disclosed which utilizes recombinant antigen, rNcp29, derived from an immunodominant surface antigen of tachyzoites. Specifically, an ELISA method is disclosed. The method provides sensitive and specific detection of antibodies in sera of infected animals and does not exhibit cross-reaction with antisera against related parasites such as . The ELISA method is used to screen animals for the presence of serum antibodies specific to recombinant Ncp29.

US Patent:

20040162418, Aug 19, 2004

Filed:

Feb 19, 2003

Appl. No.:

10/369430

Inventors:

Daniel Howe - Lexington KY, US

International Classification:

C07H021/02
C07H021/04
A61K039/002
A61K039/00

US Classification:

536/023100

Abstract:

The present invention provides novel isolated nucleic acids encoding antigenic proteins derived from , or unique fragments thereof. In particular, the invention provides novel isolated nucleic acids encoding membrane-associated polypeptides SnSAG2, SnSAG3, and SnSAG 4. Also provided are purified antigenic polypeptide fragments encoded by the novel nucleic acid sequences set forth herein that encode for . In particular, the invention provides purified antigenic polypeptide fragments encoded by the novel nucleic acid sequences set forth herein that encode for SnSAG2, SnSAG3, and SnSAG 4. Further, the invention provides a purified antigenic polypeptide fragment encoded by the nucleic acid sequences set forth herein or a selective portion thereof, in a pharmaceutically acceptable carrier. Also provided isolated nucleic acids capable of selectively hybridizing with the nucleic acid from . The invention also provides vectors comprising the nucleic acids of the invention encoding or a unique fragment thereof and provides the vector in a host capable of expressing the polypeptide encoded by that nucleic acid. Finally, the invention provides a purified polyclonal and/or a monoclonal antibody specifically reactive with and a method of detection of utilizing the antibodies of the invention.