Daniel A Keys

US Patent:

6593091, Jul 15, 2003

Filed:

Sep 24, 2001

Appl. No.:

09/962040

Inventors:

Daniel A. Keys - Irvine CA
Firdous Farooqui - Brea CA
M. Parameswara Reddy - Brea CA

Assignee:

Beckman Coulter, Inc. - Fullerton CA

International Classification:

C12Q 168

US Classification:

435 6, 435 912

Abstract:

Oligonucleotide probes, kits, and methods useful for detecting a polynucleotide target in a sample are provided. The method, a mixture is formed by combining a polynucleotide target sample, a first probe that is complementary to the polynucleotide target and having a first fluorescent donor or fluorescent acceptor; and a second probe that is partially complementary to the first probe and having a second fluorescent donor or fluorescent acceptor. The second probe competes with the polynucleotide target for binding to the first probe and the first probe preferentially binds to the polynucleotide target rather than to the second probe. The-first fluorescent donor or acceptor and second fluorescent donor or acceptor form a donor/acceptor pair capable of fluorescence resonance energy transfer (FRET) with each other in response to activation of the fluorescent donor by light of a predetermined wavelength or band of wavelengths.

US Patent:

6867005, Mar 15, 2005

Filed:

Oct 24, 2001

Appl. No.:

10/032790

Inventors:

Daniel A. Keys - Irvine CA, US
Parameswara M. Reddy - Brea CA, US

Assignee:

Beckman Coulter, Inc. - Fullerton CA

International Classification:

G01N033/53

US Classification:

435 71, 4352861, 4352872, 4352887, 436164, 436172

Abstract:

This invention relates to methods for increasing the dynamic range and accuracy of assays in which the presence, absence, activity or concentration of a target analyte is assayed by the emission or quenching of a light signal, or by a change (i. e. , an evolution or loss) of a light signal in two or more time intervals. In preferred embodiments multiple digitized images are captured at varying times, and the images analyzed to identify captured images within the dynamic range of the assay. The invention further relates to apparati capable of implementing such methods.

US Patent:

7195875, Mar 27, 2007

Filed:

Apr 18, 2003

Appl. No.:

10/419020

Inventors:

Daniel A. Keys - Irvine CA, US
Parameswara Meda Reddy - Brea CA, US
Jackie S. Bodnar - Fullerton CA, US
Firdous Farooqui - Brea CA, US

Assignee:

Beckman Coulter, Inc. - Fullerton CA

International Classification:

C12Q 1/68
C07H 21/04

US Classification:

435 6, 536 231

Abstract:

The invention is an oligonucleotide-based assay system and kit useful for sorting, detecting, and identifying analytes. The system utilizes complementary oligonucleotide pairs in which one oligonucleotide of each pair is immobilized to a solid substrate and the other oligonucleotide has an analyte binding agent attached to it. The different oligonucleotide pairs hybridize at substantially the same rate, have substantially the same Tm, have nucleotide sequences designed to minimize cross-hybridization between different pairs, and hybridize together relatively rapidly at ambient temperatures without detectable cross-hybridization.

US Patent:

7229763, Jun 12, 2007

Filed:

Apr 7, 2003

Appl. No.:

10/408626

Inventors:

M. Parameswara Reddy - Brea CA, US
Daniel A. Keys - Irvine CA, US
Firdous Farooqui - Brea CA, US

Assignee:

Beckman Coulter, Inc. - Fullerton CA

International Classification:

C12Q 1/68
G01N 33/53
C12P 19/34
C07H 21/04
A61K 39/395

US Classification:

435 6, 435 71, 435 911, 536 243, 4241301

Abstract:

The present invention provides a useful system for assays that comprises a solid support, a plurality of capture oligonucleotides immobilized onto the solid support, and complementary oligonucleotides attached to capture ligands. A detectable label can be directly attached to the capture oligonucleotides or the complementary oligonucleotides. The labeled oligonucleotides can be detected, and used to determine the quality of the assay. A labeled detector ligand corresponding to a target ligand can also be independently detected apart from the labeled oligonucleotide.

US Patent:

7368082, May 6, 2008

Filed:

Dec 12, 2002

Appl. No.:

10/317950

Inventors:

Daniel Keys - Irvine CA, US
M. Parameswara Reddy - Brea CA, US

International Classification:

G01N 15/06
G01N 33/00
G01N 33/48

US Classification:

422 681, 422 8205, 427 9, 427157

Abstract:

This invention relates to spotting solutions for the production of assay articles with uniform spot size and morphology for the detection of biopolymers, comprising N-lauroyl sarcosine in a buffered solution. Optionally the spotting solution may further comprise a fluorophore or a dye. This invention further provides means for non-destructive quality control of the production of said assay articles. This invention further provides means for non-destructive quality control of the production of said assay articles.

US Patent:

20040229226, Nov 18, 2004

Filed:

May 16, 2003

Appl. No.:

10/439811

Inventors:

M. Reddy - Brea CA, US
Kurt Brillhart - Mission Viejo CA, US
Firdous Farooqui - Brea CA, US
Daniel Keys - Irvine CA, US

International Classification:

C12Q001/68
C12M001/34

US Classification:

435/006000, 435/287200

Abstract:

The present invention provides a system using internal reference spots to reduce microarray assay variation. A microarray device for quantifying a plurality of target ligands in a sample includes a solid support having a plurality of test areas; a plurality of different target capture ligands immobilized onto the test areas; and at least one reference capture ligand immobilized onto the test areas. The reference capture ligand captures a reference ligand not normally present in the sample.

US Patent:

20050170409, Aug 4, 2005

Filed:

Feb 8, 2005

Appl. No.:

11/052219

Inventors:

Daniel Keys - Irvine CA, US
Parameswara Reddy - Brea CA, US

International Classification:

C12Q001/68
G06F019/00
G01N033/48
G01N033/50
C12M001/34

US Classification:

435006000, 702019000, 435287200

Abstract:

This invention relates to methods for increasing the dynamic range and accuracy of assays in which the presence, absence, activity or concentration of a target analyte is assayed by the emission or quenching of a light signal, or by a change (i.e., an evolution or loss) of a light signal in two or more time intervals. In preferred embodiments multiple digitized images are captured at varying times, and the images analyzed to identify captured images within the dynamic range of the assay. The invention further relates to apparati capable of implementing such methods.

US Patent:

20060292559, Dec 28, 2006

Filed:

Jun 7, 2006

Appl. No.:

11/448003

Inventors:

M. Reddy - Brea CA, US
Kurt Brillhart - Mission Viejo CA, US
Daniel Keys - Irvine CA, US

Assignee:

Beckman Coulter, Inc. - Fullerton CA

International Classification:

C12Q 1/70
C12Q 1/68
C12M 1/34

US Classification:

435005000, 435006000, 435287200, 977802000, 977924000

Abstract:

The present invention is in the field of chemistry and biotechnology. The present invention relates to cell-based microarrays, improved methods for forming such arrays, and methods for using such arrays in diagnostics, therapeutics and research. The invention particularly concerns microarrays in which ligands of a target cells are immobilized to the array support via ligand-binding molecules bound to an oligonucleotide that is hybridized to a support-immobilized oligonucleotide.