Skills:
Cloning - restriction enzyme digestion, ligation, transformation, site-directed recombination (for BAC-transgenic mouse line targeting constructs) DNA/RNA/Protein - isolation, quantification, bio-analysis, purification, electrophoresis, SDS-PAGE, PCR/qPCR cDNA library prep, primer design Microarray sample prep, basic Partek Genomic Suite analysis Imaging IHC/IF tissue cryosectioning, imaging using LSM confocal and Zeiss Apotome microscopes Live cell imaging calcium imaging, dye uptake experiments with temperature controlled liquid perfusion manipulation DAB labeling INSITU hybridization Cell Culture Mammalian cell line culture sterile technique, culturing, transfection Primary sensory neuron culture tissue dissection, cell dispersion Viral plaque assay PFU titer analysis Immunology Southern and Western blotting including duplex western blotting using a LiCoR Odyssey imager Immunoprecipitation ELISA familiar with processing human clinical samples measuring plasma cytokine levels as well as mouse serum samples measuring metabolic peptides Animal Models/methods Mouse Husbandry breeding, genotyping In vivo models - I.P. and subcutaneous drug administration, glucose tolerance test (GTT), insulin tolerance test (ITT), pancreatic islet isolation, blood sampling/glucose monitoring, general isoflurane anesthesia Tissue dissection including transcardial perfusion/fixation Behavioral pain models Hargreaves radiant heat assay, Von Frey, temperature preference, acetone evaporative cooling assay, grip strength, rotarod Computer/Data Analysis Familiarity with Novell GroupWise and Microsoft Outlook Microsoft Office - Word, Excel graph generation and statistical analysis STD,SEM, TT-TEST etc., PowerPoint extensive experience in presenting work in PowerPoint format at regular lab meetings and departmental talks Adobe Photoshop, Canvas, OriginLab, ImageJ Vector NTI - In-depth knowledge of tools for sequence analysis and alignment, primer design, plasmid construction and database organization