Daniel E Mills

US Patent:

20190054468, Feb 21, 2019

Filed:

Oct 21, 2016

Appl. No.:

15/770413

Inventors:

- Charlottesville VA, US
Kimberly Renee JACKSON - Atlanta GA, US
Daniel MILLS - Charlottesville VA, US
Gavin T. GARNER - Charlottesville VA, US
Jacquelyn A. DuVall - Raleigh NC, US
Jingyi LI - Charlottesville VA, US

International Classification:

B01L 3/00
C12Q 1/6809
C12Q 1/6816
C12Q 1/6827
G01N 33/52
G01N 33/543
G01N 33/68
G01N 35/00

Abstract:

This disclosure provides for apparatuses, systems and methods for in vitro sample detection. For example in one embodiment, this disclosure provides an automated Pe-toner microfluidic device (and related method) on a centrifugal platform for DNA sample lysis and DNA extraction. A second embodiment provides a system and method for qualitative detection, quantification, and real-time monitoring of nucleic acid amplification products using magnetic bead aggregation inhibition. A third embodiment provides a platform for simultaneous detection of mRNA markers from blood, cell-free semen, sperm, saliva, and vaginal fluid. The third embodiment comprises a system and method that provide for simple, rapid, and fluorescence-free detection of body fluids using mRNA marker amplification and optical detection for mRNA marker analysis with a smart phone with image analysis.