Darlene Snell Lawson

US Patent:

20220213496, Jul 7, 2022

Filed:

Jun 5, 2020

Appl. No.:

17/605506

Inventors:

- Lexington KY, US
- Winston-Salem NC, US
Sitakanta Pattanaik - Lexington KY, US
Darlene Madeline Lawson - Chapel Hill NC, US

International Classification:

C12N 15/82
C07K 14/415

Abstract:

A method of decreasing conversion of nicotine to nornicotine is provided herein. The methods includes administering at least one basic region/leucine zipper (bZIP) type transcription factor inhibitor to an organism in need thereof. Also provided herein is a method of decreasing conversion of nicotine to nornicotine including mutating a bZIP type transcription factor binding site on a promoter of a nicotine N-demethylase (NND). Further provided herein is a method of decreasing conversion of nicotine to nornicotine including mutating a plant genome to knockout at least one bZIP type transcription factor.