David W Allman

US Patent:

48700037, Sep 26, 1989

Filed:

Jun 15, 1987

Appl. No.:

7/061979

Inventors:

Kenneth H. Kortright - Cooper City FL
David E. Hofheinz - Homestead FL
David M. Allman - Miami FL
Meryl A. Forman - Miami FL
Song Y. Lee - Plantation FL
Paulette E. Smariga - North Miami FL
Candie S. Stoner - Hollywood FL

Assignee:

Coulter Corporation - Hialeah FL

International Classification:

G01N 33569
G01N 33535
G01N 33545

US Classification:

435 5

Abstract:

A solid-phase immunoassay is provided for determination of members of an immunological pair such as antigen and/or antibody of a single binding pair in human physiological fluid, wherein a single immunoassay enables simultaneous detection of antigen and/or antibody of a single binding pair in a test sample which may have circulating antigen and/or antibody. The immunoassay is characterized by the addition of an amount of antigen or "spike" of the binding pair to the test sample prior to incubation of the test sample in the presence of a solid-phase absorbed antibody of the binding pair. The test sample preferably also is subjected to a lysing reagent prior to the incubation for uniformly dispensing antigens which may be present in the test sample.