David Bruce Bernstein

US Patent:

51697890, Dec 8, 1992

Filed:

Dec 27, 1991

Appl. No.:

7/818439

Inventors:

David Bernstein - Sykesville MD

Assignee:

New Horizons Diagnostics Corporation - Columbia MD

International Classification:

G01N 33545

US Classification:

436501

Abstract:

A device and method for a self contained solid phase immunodiffusion assay. The device is comprised of a sample collector, and a pre-fabricated laminate which can be used in many different forms. For example, the same collector and laminate can be used with a tube having compartmentalized reagents. The seals can be broken through pressure on the sample collector. The sample collector is pushed through the seals, mixed with reagent, and then pushed into a ligand receptor reaction area which is part of the laminate. The tip of the sample collector contacts diffusable or porous membranes or filters and transfers the reactants to a capture membrane wherein a ligand receptor reaction can be examined by the naked eye or otherwise.

US Patent:

47043555, Nov 3, 1987

Filed:

Mar 27, 1985

Appl. No.:

6/716702

Inventors:

David Bernstein - Sykesville MD

Assignee:

New Horizons Diagnostics Corporation - Columbia MD

International Classification:

G01N 3353
G01N 33532

US Classification:

435 6

Abstract:

An assay utilizing receptor or antibody sensitized liposome particles which have ATP encapsulated therein. The ATP is released by lysing the liposomes, and detected by means of luciferin-luciferase reagent and a luminometer. The assay provides a very sensitive process for detecting the presence of analytes such as antigens and DNA probes.

US Patent:

D448300, Jan 26, 1999

Filed:

Nov 19, 1996

Appl. No.:

D/065444

Inventors:

Mary Ann Childs - Baltimore MD
Thomas He - Ellicott City MD
Greg K. Shipman - Catonsville MD
William Trainor - Hillsboro Beach FL
Erick Gray - Columbia MD
Mohammud Afzal Chowdhury - Columbia MD
Joseph A. McClintock - Baltimore MD
David Bernstein - Eldersburg MD

Assignee:

Universal Healthwatch, Inc. - Columbia MD

International Classification:

2402

US Classification:

D24224

US Patent:

57833990, Jul 21, 1998

Filed:

Nov 17, 1995

Appl. No.:

8/560094

Inventors:

Mary Ann Childs - Baltimore MD
Gregory K. Shipman - Baltimore MD
William P. Trainor - Hillsboro Beach FL
Erick Gray - Columbia MD
David Bernstein - Eldersburg MD

Assignee:

Universal Healthwatch, Inc. - Columbia MD

International Classification:

G01N 3353
G01N 33554
C12Q 100
C12Q 102

US Classification:

435 72

Abstract:

A method which utilize chemiluminescence for analyte detection and the detection of bacteria on surfaces. The method uses a device comprising a sampling portion made of a first adsorbent material, a container, and a second adsorbent material located within the container. The sampling portion collects analytes from a test sample which includes a surface or volume of a liquid. The second adsorbent material holds one or more chemiluminescent components including luciferase enzyme and cofactors in a dry state. In a preferred embodiment, the sampling portion is swabbed over a suspected contaminated surface. A bacteriolytic solution is then added to the adsorbent and releases ATP from any bacteria present. The ATP diffuses into the second adsorbent and mixes with the reagents therein to produce detectable light.

US Patent:

43057209, Dec 15, 1981

Filed:

Aug 20, 1979

Appl. No.:

6/068165

Inventors:

David Bernstein - Sykesville MD

Assignee:

Becton Dickinson & Company - Paramus NJ

International Classification:

G01N 3354

US Classification:

23230B

Abstract:

A gentamicin protein conjugate adsorbed on a solid support, in particular, polystyrene particles, is crosslinked by a crosslinking agent to increase the stability of the supported conjugate. The particles also generally include an adsorbed protein to increase the strength of the crosslinking network. An improved conjugate is provided by the use of orosomucoid as the protein. The sensitized particles can be employed in an agglutination inhibition card test.

US Patent:

57534977, May 19, 1998

Filed:

Dec 22, 1995

Appl. No.:

8/577105

Inventors:

David Bernstein - Eldersburg MD
Mary Ann Childs - Baltimore MD
William Trainor - Hillsboro Beach FL

Assignee:

Universal Health Watch Inc - Columbia MD

International Classification:

C12M 100

US Classification:

4352871

Abstract:

This invention provides a device and method for preparing a plasma enriched sample for use in an immunoassay strip. All parts that contact blood or a blood fraction remain in a container and prevent contamination from used blood. In one embodiment a sampling device collects blood and enters the container. A plasma enriched sample fills a plasma receptor which in turn is transferred to an immunoassay strip. Plasma is washed out of the strip by an aqueous solution. In another embodiment the sampling device doubles as a plasma separator. The invention allows sensitive and safe immunoassays to be performed on whole blood samples outside of the normal clinical laboratory setting.

US Patent:

58242686, Oct 20, 1998

Filed:

Oct 14, 1997

Appl. No.:

8/950331

Inventors:

David Bernstein - Eldersburg MD
Mary Ann Childs - Baltimore MD
William Trainor - Hillsboro Beach FL
Majorie Wier - Columbia MD
Erick Gray - Columbia MD

Assignee:

Universal Health Watch, Inc. - Columbia MD

International Classification:

G01N 33558

US Classification:

422 56

Abstract:

A test strip, a device incorporating the test strip and a method for use of the test strip within the device detecting an analyte in a solution or bodily fluid are disclosed. The test strip was three zones, a reaction zone coated with a substance that binds the analyte, a sample zone with a region for addition of the sample of solution or fluid, and a detection region zone with a region coated with detection reagent. An absorbent pad underlies the reaction zone. The reaction zone is a small membrane and is physically in contact on its upper surface with both the sample zone and the detection zone. The test strip is incorporated into a device which includes a sample collection/transferring device, a housing for holding the test strip, and a container for holding solvent solutions at both outer ends of the test strip such that the solvent solutions can come into contact with the outer ends of the test strip when pressure is applied to the ends. The method involves placing a sample onto the strip using the sample collection/transferring device, causing solvent solutions to come into contact with the sample and detection membranes, and observing the test results. The resulting orthogonal buffer flow is an improvement over current methods because it reduces the number of steps and the time required for performance of a sequential assay.

US Patent:

47708530, Sep 13, 1988

Filed:

Dec 3, 1986

Appl. No.:

6/938003

Inventors:

David Bernstein - Sykesville MD

Assignee:

New Horizons Diagnostics Corporation - Columbia MD

International Classification:

G01N 2101

US Classification:

422 58

Abstract:

A device for a self contained solid phase immunodiffusion assay. The device is comprised of a sample collector, a tube with compartmentalized reagents and a ligand receptor capture membrane filter area. The seals can be broken through pressure on the sample collector. The sample collector is pushed through the seals, mixed with reagent, and then pushed into a ligand receptor reaction area wherein the tip of the sample collector contacts diffusable membranes or filters and transfers the reactants to a capture membrane wherein a ligand receptor reaction can be visualized by the naked eye.

Address:

PO Box 833, Glen Burnie, MD 21060

Phone:

4104282626

VIN:

1D7HU18237J542111

Make:

DODGE

Model:

RAM PICKUP 1500

Year:

2007