David Alan Craft

US Patent:

6503734, Jan 7, 2003

Filed:

Jul 24, 2001

Appl. No.:

09/912161

Inventors:

David L. Craft - Fort Thomas KY
Krishna M. Madduri - Westfield IN
John C. Loper - Cincinnati OH

Assignee:

Cognis Corporation - Gulph Mills PA

International Classification:

C12D 2102

US Classification:

435 691, 536 232, 43525411, 4352554, 4353201

Abstract:

A novel gene has been isolated which encodes cytochrome b5 (CYTb5) protein of the -hydroxylase complex of 20336. Vectors including this gene, and transformed host cells are provided. Methods of increasing the production of a CYTb5 protein are also provided which involve transforming a host cell with a gene encoding this protein and culturing the cells. Methods of increasing the production of a dicarboxylic acid are also provided which involve increasing in the host cell the number of genes encoding this protein.

US Patent:

7049112, May 23, 2006

Filed:

May 3, 2002

Appl. No.:

10/139296

Inventors:

C. Ron Wilson - Loveland OH, US
David L. Craft - Fort Thomas KY, US
L. Dudley Eirich - Cincinnati OH, US
Mark Eshoo - Fairfax CA, US
Krishna M. Madduri - Westfield IN, US
Cathy A. Cornett - Crescent Springs KY, US
Alfred A. Brenner - Santa Rosa CA, US
Maria Tang - Fairfield CA, US
John C. Loper - Cincinnati OH, US
Martin Gleeson - San Diego CA, US

Assignee:

Cognis Corporation - Ambler PA

International Classification:

C12N 9/02
C12N 15/00
C12N 1/20
C07H 21/04
C12P 21/04

US Classification:

435189, 435 691, 435 711, 4352523, 4353201, 536 232, 536 2374

Abstract:

Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the ω-hydroxylase complex of 20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.

US Patent:

7063972, Jun 20, 2006

Filed:

May 3, 2002

Appl. No.:

10/138838

Inventors:

C. Ron Wilson - Loveland OH, US
David L. Craft - Fort Thomas KY, US
L. Dudley Eirich - Cincinnati OH, US
Mark Eshoo - Fairfax CA, US
Krishna M. Madduri - Westfield IN, US
Cathy A. Cornett - Crescent Springs KY, US
Alfred A. Brenner - Santa Rosa CA, US
Maria Tang - Fairfield CA, US
John C. Loper - Cincinnati OH, US
Martin Gleeson - San Diego CA, US

Assignee:

Cognis Corporation - Ambler PA

International Classification:

C12N 9/02
C12N 15/00
C12N 1/20
C12P 21/04
C07H 21/04

US Classification:

435189, 435 691, 435 711, 4352523, 4353201, 536 232, 536 2374

Abstract:

Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the ω-hydroxylase complex of 20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.

US Patent:

7109009, Sep 19, 2006

Filed:

Apr 2, 2003

Appl. No.:

10/405660

Inventors:

C. Ron Wilson - Loveland OH, US
David L. Craft - Fort Thomas KY, US
L. Dudley Eirich - Cincinnati OH, US
Mark Eshoo - Fairfax CA, US
Krishna M. Madduri - Westfield IN, US
Cathy A. Cornett - Crescent Springs KY, US
Alfred A. Brenner - Santa Rosa CA, US
Maria Tang - Fairfield CA, US
John C. Loper - Cincinnati OH, US
Martin Gleeson - San Diego CA, US

Assignee:

Cognis Corporation - Ambler PA

International Classification:

C12N 9/02
C12N 1/20
C12N 15/00
C12Q 1/00
C12Q 1/68
C07H 21/02
C07H 21/04
C12P 21/04

US Classification:

435189, 435 4, 435 6, 4352523, 4353201, 435440, 435 691, 435 711, 536 231, 536 232, 536 237

Abstract:

Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the ω-hydroxylase complex of 20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.

US Patent:

7160708, Jan 9, 2007

Filed:

Apr 18, 2003

Appl. No.:

10/418819

Inventors:

L. Dudley Eirich - Milford OH, US
David L. Craft - Fort Thomas KY, US

Assignee:

Cognis Corporation - Ambler PA

International Classification:

C12N 9/02
C12N 15/00
C12N 1/20
C12P 21/04
C12Q 1/00
C12Q 1/34
C07H 21/04
C12Q 1/26
C12Q 1/68

US Classification:

435189, 435 4, 435 6, 435 18, 435 25, 4352523, 4353201, 435440, 435 691, 435 711, 536 232, 536 237

Abstract:

The present invention provides fatty alcohol oxidase (FAO) proteins and nucleic acid molecules encoding the FAO proteins. Also provided are analogs, derivatives, and enzymatically active fragments of the FAO proteins. Vectors and host cells comprising the nucleic acid molecules encoding the FAO proteins, analogs, derivatives and enzymatically active fragments thereof are also provided. In addition, FAO signature peptides and isolated nucleic acid molecules encoding the signature peptides are provided by the present invention. Methods of producing or increasing production of a subject FAO protein, methods for increasing aldehyde production during the second step of the ω-oxidation pathway of fatty acids, methods for increasing production of a ketone from an alcohol during the second step of the ω-oxidation pathway of fatty acids, and methods for increasing production of a dicarboxylic acid are also provided.

US Patent:

20030049822, Mar 13, 2003

Filed:

May 3, 2002

Appl. No.:

10/139031

Inventors:

C. Wilson - Loveland OH, US
David Craft - Fort Thomas KY, US
L. Eirich - Cincinnati OH, US
Mark Eshoo - Fairfax CA, US
Krishna Madduri - Westfield IN, US
Cathy Cornett - Crescent Springs KY, US
Alfred Brenner - Santa Rosa CA, US
Maria Tang - Fairfield CA, US
John Loper - Cincinnati OH, US
Martin Gleeson - San Diego CA, US

International Classification:

C12N009/02
C07H021/04
C12P021/02
C12N001/18
C12N015/74

US Classification:

435/189000, 435/069100, 435/320100, 435/254220, 536/023200, 435/483000

Abstract:

Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the -hydroxylase complex of 20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.

US Patent:

20030068800, Apr 10, 2003

Filed:

May 3, 2002

Appl. No.:

10/138905

Inventors:

C. Wilson - Loveland OH, US
David Craft - Fort Thomas KY, US
L. Eirich - Cincinnati OH, US
Mark Eshoo - Fairfax CA, US
Krishna Madduri - Westfield IN, US
Cathy Cornett - Crescent Springs KY, US
Alfred Brenner - Santa Rosa CA, US
Maria Tang - Fairfield CA, US
John Loper - Cincinnati OH, US
Martin Gleeson - San Diego CA, US

International Classification:

C12N009/02
C12N001/18
C07H021/04
C12P021/02
C12N015/74

US Classification:

435/189000, 435/320100, 435/069100, 435/254220, 536/023200

Abstract:

Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the -hydroxylase complex of 20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.

US Patent:

20030073220, Apr 17, 2003

Filed:

May 3, 2002

Appl. No.:

10/138916

Inventors:

C. Wilson - Loveland OH, US
David Craft - Fort Thomas KY, US
L. Eirich - Cincinnati OH, US
Mark Eshoo - Fairfax CA, US
Krishna Madduri - Westfield IN, US
Cathy Cornett - Crescent Springs KY, US
Alfred Brenner - Santa Rosa CA, US
Maria Tang - Fairfield CA, US
John Loper - Cincinnati OH, US
Martin Gleeson - San Diego CA, US

International Classification:

C12N009/02
C07H021/04
C12N001/18
C12P021/02
C12N015/74

US Classification:

435/189000, 435/320100, 435/069100, 536/023200, 435/254220

Abstract:

Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the -hydroxylase complex of 20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.