David Simon Dahan

US Patent:

20070161052, Jul 12, 2007

Filed:

Oct 19, 2006

Appl. No.:

11/583086

Inventors:

Guy Servant - San Diego CA, US
Mark Williams - Carlsbad CA, US
Andrew Patron - San Marcos CA, US
Poonit Kamdar - San Diego CA, US
Qing Chen - San Diego CA, US
Bryan Moyer - San Diego CA, US
David Dahan - Oceanside CA, US
Tanya Ditschun - San Diego CA, US
Sumita Ray - San Diego CA, US
Amy Ligani - San Diego CA, US

Assignee:

SENOMYX, INC. - LaJolla CA

International Classification:

G01N 33/567

US Classification:

435007200

Abstract:

Robust cell based assays are provided that use cells that express wild-type or modified TRPM5 nucleic acid sequences in order to identify putative taste modulators, preferably sweet, bitter and umami taste modulators. The preferred assays use HEK-293 cells that express TRPM5, optionally at least one GPCR, preferably a taste specific GPCR, and a G protein that couples therewith. These assays detect TRPM5 modulators by use of membrane potential dyes that emit fluorescence on changes in TRPM5 activity based on changes in membrane potential and these changes in fluorescence are detectable using Fluorimetric Imaging Plate Readers (FLIPR).

US Patent:

20070259354, Nov 8, 2007

Filed:

Sep 28, 2006

Appl. No.:

11/536394

Inventors:

Guy Servant - San Diego CA, US
Paul Brust - San Diego CA, US
Bryan Moyer - San Diego CA, US
Min Lu - San Diego CA, US
Fernando Echeverri - Chula Vista CA, US
David Dahan - Oceanside CA, US
Mark Zoller - La Jolla CA, US
Mark Williams - Carlsbad CA, US
Rachel Kimmich - Carlsbad CA, US
Poonit Kamdar - San Diego CA, US
Tanya Ditschun - San Diego CA, US
Andrew Patron - San Marcos CA, US

Assignee:

Senomyx, Inc. - San Diego CA

International Classification:

C12Q 1/68
C07H 21/02
C07H 21/04
C12N 15/85
C12N 5/10

US Classification:

435006000, 435320100, 435358000, 435363000, 435365000, 435366000, 435367000, 536023100, 536024100

Abstract:

Modified human TRPM8 nucleic acid sequences which are efficiently expressed in human cells and cell-based assays and test kits containing same are provided. These assays identify TRPM8 modulators using cells that express a modified human TRPM8 nucleic acid sequence according to the invention, wherein said sequence has been modified relative to a wild-type human TRPM8 nucleic acid sequence in order to optimize ion channel expression in desired cells. Assays using these modified TRPM8 sequences have been shown to identify compounds that modulate the human TRPM8 ion channel better or comparably to known coolants such as menthol and icilin.