Skills:
ligand-binding assay development (ELISA, MSD Sector Imager, Gyrolab), radiolabeling of proteins and nucleic acids, cell-based assays, Western blot, eukaryotic tissue culture, hybridoma cultivation, host cell protein assays, radioimmunoassay, cAMP cellular assays, intracellular calcium flux assays, analysis of biological samples from radiodosed animals, cytotoxicity assay, RNA isolation from eukaryotic cell cultures, cDNA preparation, electrophoresis of nucleic acids and SDS-PAGE, densitometry, TLC, Ficoll Paque sedimentation, PCR, sequencing of Ig genes, small animal handling, protein purification, FACS analysis (with CellQuest software), Staclot, bacterial growth and transformation, plasmid preparation, HPLC, dosing formulation for in vivo studies, SAS programming, SOP generation, MS Excel, MS Word, Graphpad Prism, and Minitab