David A Lenz

US Patent:

6406876, Jun 18, 2002

Filed:

Apr 26, 2000

Appl. No.:

09/558511

Inventors:

Richard K. Gordon - Potomac MD
Bhupendra P. Doctor - Potomac MD
Ashima Saxena - Fairfax VA
Shawn R. Feaster - Damascus MD
Donald Maxwell - Baltimore MD
Michelle Ross - Edgewood MD
David Lenz - Bel Air MD
Keith LeJeune - Pittsburgh PA
Alan Russell - Wexford PA

Assignee:

The United States of America as represented by the Secretary of the Army - Washington DC

International Classification:

C12Q 146

US Classification:

435 20, 435 19, 435 28, 435 18, 435 25, 4352891, 4352831, 4352871, 4352865, 435963, 435970

Abstract:

Methods, compositions and materials useful in the detection of organophosphorous and organosulfur compounds are disclosed. In particular, biosensors wherein a porous or a non-porous support having an enzyme immobilized upon or within are disclosed. The biosensors exhibit enzymatic stability at extreme temperatures and/or denaturing conditions, and similar kinetic characteristics of the soluble form of the enzymes utilized. The enzyme does not leach from the porous or non-porous support and the material retains enzymatic activity after prolonged storage. Differential biosensors are also disclosed.

US Patent:

6642037, Nov 4, 2003

Filed:

Apr 26, 2000

Appl. No.:

09/558512

Inventors:

Richard K. Gordon - Potomac MD
Bhupendra P. Doctor - Potomac MD
Ashima Saxena - Fairfax VA
Shawn R. Feaster - Damascus MD
Donald Maxwell - Baltimore MD
Michelle Ross - Edgewood MD
David Lenz - Bel Air MD
Keith Lejeune - Pittsburgh PA
Alan Russell - Wexford PA

Assignee:

The United States of America as represented by the Secretary of the Army - Washington DC

International Classification:

C12N 1104

US Classification:

435182, 435180, 4352625

Abstract:

An enzymatically active reusable sponge or foam, capable of regeneration with oximes, made of a polymer such as polyurethane is prepared for detoxification of hazardous compounds such as organophosphorus and organosulfur compounds. The foam or sponge contains a plurality of enzymes including enzymes selected from acetylcholinesterase, butyrylcholinesterase, triesterase, pseudocholinesterase, organophosphate hydrase, phosphotriesterase, paraoxonase and organophosphorus and organosulfur hydrolyzing enzymes. The sponge or foam may additionally contain activated carbon and an enzyme reactivation compound. A kit can be formed containing the sponge or foam and the compound for enzyme reactivation. The enzymatically active foam or sponge may be prepared using a two chamber device where enzymes and prepolymer are passed from separate chambers into a static mixing stator and are subjected to low shear mixing and extrusion to form by the sponge or foam.

US Patent:

7754461, Jul 13, 2010

Filed:

Apr 10, 2007

Appl. No.:

11/733246

Inventors:

Bhupendra P. Doctor - Potomac MD, US
Ashima Saxena - Fairfax VA, US
Wei Sun - Gaithersburg MD, US
Chunyuan Luo - North Potomac MD, US
Prasanthi Tipparaju - Rockville MD, US
Irwin Koplovitz - Bel Air MD, US
David E. Lenz - Belcamp MD, US
Michelle C. Ross - Edgewood MD, US

Assignee:

The United States of America as represented by the Secretary of the Army - Washington DC

International Classification:

C12N 9/16
C12N 9/14
C12N 9/00
A61K 38/46

US Classification:

435196, 435183, 435195, 424 946

Abstract:

Disclosed herein are methods for the large-scale preparation of human butyrylcholinesterase (HuBChE) preparations from Cohn Fraction IV-4. As disclosed, the methods provide HuBChE preparations that are about 99% or more pure with recovery yields of about 60%. Also disclosed are the pharmacokinetics, safety and toxicity, stability and efficacy of the HuBChE preparations.

US Patent:

20040005681, Jan 8, 2004

Filed:

Jul 2, 2003

Appl. No.:

10/610742

Inventors:

Richard Gordon - Potomac MD, US
Bhupendra Doctor - Potomac MD, US
Ashima Saxena - Fairfax VA, US
Shawn Feaster - Damascus MD, US
Donald Maxwell - Baltimore MD, US
Michelle Ross - Edgewood MD, US
David Lenz - Bel Air MD, US
Keith LeJeune - Pittsburgh PA, US
Alan Russell - Wexford PA, US

International Classification:

C12N011/18

US Classification:

435/175000

Abstract:

A material comprising a porous support and a plurality of enzymes for the removal, decontamination or neutralization of hazardous chemicals such as OP compounds is disclosed. The material may be used on a variety of surfaces, including natural, synthetic and biological surfaces such as skin and other delicate membranes. Also disclosed is a process of making the material, kits and various methods and reactivation devices for reactivating the enzymatic activity of the material.

US Patent:

20060194301, Aug 31, 2006

Filed:

Oct 6, 2005

Appl. No.:

11/244173

Inventors:

Bhupendra Doctor - Potomac MD, US
Ashima Saxena - Fairfax VA, US
Wei Sun - Gaithersburg MD, US
Chunyuan Luo - North Potomac MD, US
Prasanthi Tipparaju - Rockville MD, US
Irwin Koplovitz - Bel Air MD, US
David Lenz - Belcamp MD, US
Michelle Ross - Edgewood MD, US

International Classification:

C12N 9/00
C12N 9/16

US Classification:

435196000, 435183000

Abstract:

Disclosed herein are methods for the large-scale preparation of human butyrylcholinesterase (HuBChE) preparations from Cohn Fraction IV-4. As disclosed, the methods provide HuBChE preparations that are about 99% or more pure with recovery yields of about 60%. Also disclosed are the pharmacokinetics, safety and toxicity, stability and efficacy of the HuBChE preparations.