David Y Light

US Patent:

8574835, Nov 5, 2013

Filed:

May 24, 2010

Appl. No.:

12/785685

Inventors:

Wolfgang Hinz - New Haven CT, US
John Leamon - Clinton CT, US
David Light - Guilford CT, US
Jonathan Rothberg - Guilford CT, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

C12Q 1/68
C12M 1/00
C12M 1/34
C12M 3/00

US Classification:

435 61, 4352831, 4352871, 4352872, 257253

Abstract:

The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

US Patent:

20110195252, Aug 11, 2011

Filed:

Feb 14, 2011

Appl. No.:

13/026707

Inventors:

Wolfgang HINZ - New Haven CT, US
John Leamon - Guilford CT, US
David Light - Guilford CT, US
Jonathan Rothberg - Guilford CT, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

B32B 5/00
C08F 265/10

US Classification:

428402, 525 542

Abstract:

The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

US Patent:

20110195253, Aug 11, 2011

Filed:

Feb 14, 2011

Appl. No.:

13/026759

Inventors:

Wolfgang HINZ - New Haven CT, US
John LEAMON - Guilford CT, US
David LIGHT - Guilford CT, US
Jonathan ROTHBERG - Guilford CT, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

B32B 5/00
C08F 265/10

US Classification:

428402, 525 543

Abstract:

The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

US Patent:

20110195459, Aug 11, 2011

Filed:

Feb 17, 2011

Appl. No.:

13/029664

Inventors:

Wolfgang HINZ - New Haven CT, US
John Leamon - Guilford CT, US
David Light - Guilford CT, US
Jonathan Rothberg - Guilford CT, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12P 19/34

US Classification:

435 912

Abstract:

The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

US Patent:

20110201506, Aug 18, 2011

Filed:

Feb 17, 2011

Appl. No.:

13/029611

Inventors:

Wolfgang HINZ - New Haven CT, US
John Leamon - Guilford CT, US
David Light - Guilford CT, US
Jonathan Rothberg - Guilford CT, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C40B 10/00
C40B 50/00

US Classification:

506 1, 506 23

Abstract:

The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

US Patent:

20110201508, Aug 18, 2011

Filed:

Feb 17, 2011

Appl. No.:

13/029566

Inventors:

WOLFGANG HINZ - NEW HAVEN CT, US
JOHN LEAMON - GUILFORD CT, US
DAVID LIGHT - Guilford CT, US
JONATHAN ROTHBERG - GUILFORD CT, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C40B 30/00
C12Q 1/68

US Classification:

506 7, 435 61

Abstract:

The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

US Patent:

20110201523, Aug 18, 2011

Filed:

Feb 15, 2011

Appl. No.:

13/027336

Inventors:

Wolfgang HINZ - New Haven CT, US
John Leamon - Guilford CT, US
David Light - Guilford CT, US
Jonathan Rothberg - Guilford CT, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C40B 40/06

US Classification:

506 16

Abstract:

The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

US Patent:

20110318820, Dec 29, 2011

Filed:

Jun 29, 2011

Appl. No.:

13/172048

Inventors:

Wolfgang Hinz - Killingworth CT, US
David Light - New Haven CT, US
Todd Rearick - Cheshire CT, US
James Ball - Ledyard CT, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12M 1/34

US Classification:

4352872

Abstract:

The disclosure relates to novel particle compositions and methods of making said compositions having applications in nucleic acid analysis, as well as apparatuses and systems for the same.