David G Lockhart

US Patent:

6391550, May 21, 2002

Filed:

May 27, 1999

Appl. No.:

09/321481

Inventors:

David J. Lockhart - Del Mar CA
Gordon G. Wong - Brookline MA

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C12Q 168

US Classification:

435 6, 536 231, 536 243, 935 76, 935 77, 935 78

Abstract:

Novel means and methods for analyzing hybridization data derived from hybridization assays between a target nucleic acid and differently sequenced polynucleotide probes involve selecting probe sets that define reference sequences for sequence signatures and deriving useful data about the nature of the target nucleic acid molecule based on its hybridization to the probes. The methods are useful for determining whether the target contains a nucleic acid or polypeptide sequence signature, whether the target encodes a member of a gene family, or whether the target is derived from one of any number of genes.

US Patent:

6524800, Feb 25, 2003

Filed:

Jul 6, 2001

Appl. No.:

09/900845

Inventors:

David J. Lockhart - Del Mar CA
Lisa Wodicka - San Diego CA
Ming Hsiu Ho - San Jose CA

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C12Q 168

US Classification:

435 6, 435 4, 435 71, 435 912, 435288, 536 231, 536 243

Abstract:

The cellular effects of potentially therapeutic compounds are characterized in mammalian cells and yeast. In the latter case the effects can be characterized on a genome-wide scale by monitoring changes in messenger RNA levels in treated cells with high-density oligonucleotide probe arrays.

US Patent:

6638719, Oct 28, 2003

Filed:

Jun 26, 2000

Appl. No.:

09/603999

Inventors:

Kevin Gunderson - San Diego CA
David J. Lockhart - Del Mar CA

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C12Q 168

US Classification:

435 6, 435 4, 435 911, 435 912, 536 231, 536 235, 536 243, 536 2433

Abstract:

A method is described for determining the genotype of one or more individuals at a biallelic marker. The method employs amplification of a region of genomic DNA using color tagged, allele-specific primers and hybridization of the products to an array of allele-specific probes. The genotype is identified from the pattern of hybridization.

US Patent:

6927032, Aug 9, 2005

Filed:

Jan 28, 2003

Appl. No.:

10/353792

Inventors:

David J. Lockhart - Del Mar CA, US
Eugene L. Brown - Newton Highlands MA, US
Gordon G. Wong - Brookline MA, US
Mark Chee - Palo Alto CA, US
Thomas R. Gingeras - Encinitas CA, US

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C12Q001/68
C12P019/34
C12M001/34
C07H021/02
C07H021/04

US Classification:

435 6, 435 911, 4352811, 4352812, 536 231, 536 243

Abstract:

This invention provides methods of monitoring the expression levels of a multiplicity of genes. The methods involve hybridizing a nucleic acid sample to a high density array of oligonucleotide probes where the high density array contains oligonucleotide probes complementary to subsequences of target nucleic acids in the nucleic acid sample. In one embodiment, the method involves providing a pool of target nucleic acids comprising RNA transcripts of one or more target genes, or nucleic acids derived from the RNA transcripts, hybridizing said pool of nucleic acids to an array of oligonucleotide probes immobilized on surface, where the array comprising more than 100 different oligonucleotides and each different oligonucleotide is localized in a predetermined region of the surface, the density of the different oligonucleotides is greater than about 60 different oligonucleotides per 1 cm, and the olignucleotide probes are complementary to the RNA transcripts or nucleic acids derived from the RNA transcripts; and quantifying the hybridized nucleic acids in the array.

US Patent:

7112435, Sep 26, 2006

Filed:

Aug 7, 2002

Appl. No.:

10/214654

Inventors:

Pietro Ciceri - La Jolla CA, US
Patrick Parvis Zarrinkar - San Diego CA, US
Daniel Kelly Treiber - San Diego CA, US
David J. Lockhart - Del Mar CA, US

Assignee:

Ambit Biosciences Corporation - San Diego CA

International Classification:

C12N 15/62
C12Q 1/68
C12Q 1/70
C07H 21/04

US Classification:

4353201, 435 5, 435 6, 435472, 435475, 435476, 536 234

Abstract:

The present invention provides an advance in phage display technology by permitting the uncoupling of the propagation of phages containing inserted sequences encoding heterologous polypeptides from the expression of said polypeptides. The invention provides phage constructs and methods for their use to permit phage coat protein expression, and thus phage propagation, in the absence of display of heterologous polypeptides, which may be expressed as a fusion with said coat protein in a regulated manner.

US Patent:

7135285, Nov 14, 2006

Filed:

Mar 14, 2002

Appl. No.:

10/099442

Inventors:

David J. Lockhart - Del Mar CA, US
Gordon G. Wong - Brookline MA, US
Pennina Langer-Safer - Rehovot, IL

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C12Q 1/68
C07H 21/02
C07H 21/04

US Classification:

435 6, 536 231, 536 243

Abstract:

Novel means and methods for analyzing hybridization data derived from hybridization assays between a target nucleic acid and differently sequenced polynucleotide probes involve selecting probe sets that define reference sequences for sequence signatures and deriving useful data about the nature of the target nucleic acid molecule based on its hybridization to the probes. The methods are useful for determining whether the target contains a nucleic acid or polypeptide sequence signature, whether the target encodes a member of a gene family, or whether the target is derived from one of any number of genes.

US Patent:

7323307, Jan 29, 2008

Filed:

Aug 4, 2004

Appl. No.:

10/911065

Inventors:

David J. Lockhart - Del Mar CA, US
Gordon G. Wong - Brookline MA, US
Pennina Langer-Safer - Rehovot, IL

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C12Q 1/68
C07H 21/02
C07H 21/04

US Classification:

435 6, 536 231, 536 243

Abstract:

Novel means and methods for analyzing hybridization data derived from hybridization assays between a target nucleic acid and differently sequenced polynucleotide probes involve selecting probe sets that define reference sequences for sequence signatures and deriving useful data about the nature of the target nucleic acid molecule based on its hybridization to the probes. The methods are useful for determining whether the target contains a nucleic acid or polypeptide sequence signature, whether the target encodes a member of a gene family, or whether the target is derived from one of any number of genes.

US Patent:

7323490, Jan 29, 2008

Filed:

May 17, 2004

Appl. No.:

10/848584

Inventors:

David J. Lockhart - Del Mar CA, US
Hitesh K. Patel - Encinitas CA, US
Zdravko V. Milanov - San Diego CA, US
Shamal Anil Mehta - San Diego CA, US
Patrick Parvis Zarrinkar - San Diego CA, US
Pietro Ciceri - La Jolla CA, US
Miles A. Fabian - La Jolla CA, US
Daniel K. Treiber - San Diego CA, US

Assignee:

Ambit Biosciences Corporation - San Diego CA

International Classification:

A61K 31/40
C07D 207/32

US Classification:

514423, 514427, 514428, 514429, 548537, 548561, 548563

Abstract:

The invention provides pyrrole-containing compounds and methods of use thereof. Kits and pharmaceutical compositions comprising the pyrrole compounds of the invention are also provided. The compounds and compositions disclosed herein are preferably used in the treatment of neurodegenerative diseases, cardiovascular diseases, proliferative diseases, and visual disorders. In particular, methods and compositions for the treatment of stroke are disclosed herein.