David Alan Lockhart

US Patent:

6573044, Jun 3, 2003

Filed:

Dec 22, 1998

Appl. No.:

09/221406

Inventors:

Nathanael S. Gray - Berkeley CA
Peter Schultz - Oakland CA
Lisa Wodicka - Santa Clara CA
Laurent Meijer - Roscoff, FR
David J. Lockhart - Mountain View CA

Assignee:

The Regents of the University of California - Oakland CA
Affymetrix, Inc. - Santa Clara CA
Centre National de la Recherche Scientifique - Paris

International Classification:

C12Q 168

US Classification:

435 6, 435 912, 435DIG 17, 544268, 544276, 544277

Abstract:

The generation of selective inhibitors for specific protein kinases would provide new tools for analyzing signal transduction pathways and possibly new therapeutic agents. We have invented an approach to the development of selective protein kinase inhibitors based on the unexpected binding mode of 2,6,9-trisubstituted purines to the ATP binding site of human CDK2. The most potent inhibitor, purvalanol B (IC =6 nM), binds with a 30-fold greater affinity than the known CDK2 inhibitor, flavopiridol. The cellular effects of this class of compounds were examined and compared to those of flavopiridol by monitoring changes in mRNA expression levels for all genes in treated cells of using high-density oligonucleotide probe arrays.

US Patent:

6582906, Jun 24, 2003

Filed:

Apr 5, 1999

Appl. No.:

09/285658

Inventors:

Yanxiang Cao - Palo Alto CA
David J. Lockhart - Mountain View CA
Rui Mei - Santa Clara CA
Xing Su - Cupterino CA

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C12Q 168

US Classification:

435 6, 435 912, 435 9151

Abstract:

The proportional amplification of nucleic acids can increase the amount of nucleic acids while preserving the relative abundance of the individual nucleic acid species, or portions thereof, in the original sample. A proportionally amplified nucleic acid preparation may be analyzed in a gene expression monitoring system, preferably involving a nucleic acid probe array.

US Patent:

6821724, Nov 23, 2004

Filed:

Sep 15, 1999

Appl. No.:

09/396196

Inventors:

Michael Mittman - Palo Alto CA
David J. Mack - Menlo Park CA
David J. Lockhart - Del Mar CA

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C12Q 168

US Classification:

435 6, 422 681, 536 231, 536 243, 536 2431

Abstract:

The invention provides nucleic acid sequences which are complementary, in one embodiment, to a wide variety of murine genes. The invention provides the sequences in such a way as to make them available for a variety of analyses. As such, the invention related to diverse fields impacted by the nature of molecular interaction, including chemistry, biology, medicine, and medical diagnostics.

US Patent:

7157564, Jan 2, 2007

Filed:

Apr 4, 2001

Appl. No.:

09/827383

Inventors:

Michael Mittmann - Palo Alto CA, US
MacDonald Morris - Felton CA, US
Thomas B. Ryder - Los Gatos CA, US
David Lockhart - Del Mar CA, US

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C07H 21/02

US Classification:

536 231

Abstract:

The invention provides a unique set of nucleic acid sequences which is appropriate for use for a wide variety of applications requiring nucleic acid tags. As such, the sequence tags of the presently claimed invention may be used, for example, to label biological and nonbiological materials, in genotyping applications and in a variety of other analyses.

US Patent:

20030203399, Oct 30, 2003

Filed:

Jun 18, 2003

Appl. No.:

10/465363

Inventors:

Yanxiang Cao - Palo Alto CA, US
David Lockhart - Mountain View CA, US
Rui Mei - Santa Clara CA, US
Xing Su - Cupertino CA, US

International Classification:

C12Q001/68
C12P019/34
C07H021/04

US Classification:

435/006000, 435/091200, 536/023100

Abstract:

The proportional amplification of nucleic acids can increase the amount of nucleic acids while preserving the relative abundance of the individual nucleic acid species, or portions thereof, in the original sample. A proportionally amplified nucleic acid preparation may be analyzed in a gene expression monitoring system, preferably involving a nucleic acid probe array.

US Patent:

20050048531, Mar 3, 2005

Filed:

Mar 25, 2004

Appl. No.:

10/809189

Inventors:

Michael Mittman - Palo Alto CA, US
David Mack - Menlo Park CA, US
David Lockhart - Del Mar CA, US

Assignee:

Affymetrix, INC. - Santa Clara CA

International Classification:

C12Q001/68
C12M001/34

US Classification:

435006000, 435287200

Abstract:

The invention provides nucleic acid sequences which are complementary, in one embodiment, to a wide variety of murine genes. The invention provides the sequences in such a way as to make them available for a variety of analyses. As such, the invention related to diverse fields impacted by the nature of molecular interaction, including chemistry, biology, medicine, and medical diagnostics.

US Patent:

20060024658, Feb 2, 2006

Filed:

Dec 23, 2003

Appl. No.:

10/746794

Inventors:

Michael Miles - Mill Valley CA, US
Chao-Qiang Lai - Belmont MA, US
David Lockhart - Del Mar CA, US

International Classification:

C12Q 1/00
C12Q 1/68

US Classification:

435004000, 435006000

Abstract:

This invention pertains to the identification of genes whose expression levels are altered by chronic exposure of a cell, tissue, or organism to one or more drugs of abuse (e.g. alcohol, stimulants, opiates, etc.). In one embodiment, this invention provides a method of monitoring the response of a cell a drug of abuse. The method involves contacting the cell with the drug of abuse; providing a biological sample comprising the cell; and detecting, in the sample, the expression of one or more genes or ESTs identified herein, where a difference between the expression of one or more of said genes or ESTs in said sample and one or more of said genes or ESTs in a biological sample not contacted with said drug of abuse indicates a response of the cell to the drug of abuse

US Patent:

20080032408, Feb 7, 2008

Filed:

Nov 22, 2006

Appl. No.:

11/562548

Inventors:

Michael Mittmann - Palo Alto CA, US
MacDonald Morris - Felton CA, US
Thomas B. Ryder - Los Gatos CA, US
David Lockhart - Del Mar CA, US

Assignee:

Affymetrix, INC. - Santa Clara CA

International Classification:

G01N 33/00

US Classification:

436 56

Abstract:

The invention provides a unique set of nucleic acid sequences which is appropriate for use for a wide variety of applications requiring nucleic acid tags. As such, the sequence tags of the presently claimed invention may be used, for example, to label biological and nonbiological materials, in genotyping applications and in a variety of other analyses.