David A Lockhart

US Patent:

20050074787, Apr 7, 2005

Filed:

Dec 8, 2003

Appl. No.:

10/730771

Inventors:

Joel Hirschhorn - Newton MA, US
Xiaohua Huang - Mountain View CA, US
Paul Kaplan - Campbell CA, US
Eric Lander - Cambridge MA, US
David Lockhart - Del Mar CA, US
Thomas Ryder - Los Gatos CA, US
Pamela Sklar - Brookline MA, US

Assignee:

Whitehead Institute for Biomedical Research - Cambridge MA
The General Hospital Corporation - Boston MA
Affymetrix, Inc - Santa Clara CA

International Classification:

C12Q001/68

US Classification:

435006000

Abstract:

An array of oligonucleotides on a solid substrate is disclosed, which can be used for multiple purposes. Methods and reagents are provided for performing genotyping to determine the identity or ratio of allelic forms of a gene in a sample. A single base extension primer is coupled to a sequence identity code. During the primer extension reaction a distinctive label is incorporated which identifies the allelic form present in the sample. This permits multiple simultaneous analyses to be performed easily and efficiently.