David P Mcgee

US Patent:

6426185, Jul 30, 2002

Filed:

Jul 21, 1999

Appl. No.:

09/359301

Inventors:

Monto H. Kumagai - Davis CA
Guy R. della-Cioppa - Vacaville CA
Robert L. Erwin - Vacaville CA
David R. McGee - Vacaville CA

Assignee:

Large Scale Biology Corporation - Vacaville CA

International Classification:

C12Q 168

US Classification:

435 6, 435 911, 435468, 536 231, 536 236, 536 2372, 536 241, 536 245

Abstract:

The present invention provides a method of compiling a plant functional gene profile, a method of changing the phenotype or biochemistry of a plant, a method of determining a change in phenotype or biochemistry of a plant, and a method of determining the presence of a trait in plant. The methods comprise expressing transiently a nucleic acid sequence of a plant into a host plant to affect phenotypic or biochemical changes in the host plant. A viral vector functional genomic screen has been developed to identify nucleotide sequences in transfected plants by systemically knocking out endogenous gene expression in an antisense mechanism. Once the presence of a trait in a plant is identified by phenotypic or biochemical changes in the host plant, the nucleic acid insert in the cDNA clone or in the vector that results in the changes is then sequenced. The present invention exemplifies that genes encoding GTP binding proteins in one plant can silence endogenous gene expression in a different plant.

US Patent:

20030024008, Jan 30, 2003

Filed:

Apr 24, 2002

Appl. No.:

10/133934

Inventors:

Monto Kumagai - Davis CA, US
Guy Della-Cioppa - Vacaville CA, US
Robert Erwin - Vacaville CA, US
David McGee - Vacaville CA, US

International Classification:

A01H005/00

US Classification:

800/278000, 800/320200

Abstract:

The present invention provides a method of compiling a plant functional gene profile, a method of changing the phenotype or biochemistry of a plant, a method of determining a change in phenotype or biochemistry of a plant, a method of determining the presence of a trait in plant, and a method of increasing grain crop. The methods comprise expressing transiently a nucleic acid sequence of a plant into a host plant to affect phenotypic or biochemical changes in the host plant. A viral vector functional genomic screen has been developed to identify nucleotide sequences in transfected plants by systemically knocking out endogenous gene expression in an antisense mechanism. Once the presence of a trait in a plant is identified by phenotypic or biochemical changes in the host plant, the nucleic acid insert in the cDNA clone or in the vector that results in the changes is then sequenced. The present invention exemplifies that genes encoding GTP binding proteins in one plant can silence endogenous gene expression in a different plant.

US Patent:

20030027173, Feb 6, 2003

Filed:

Feb 5, 2002

Appl. No.:

10/072438

Inventors:

Guy Della-Cioppa - Vacaville CA, US
Robert Erwin - Vacaville CA, US
Wayne Fitzmaurice - Vacaville CA, US
Kathleen Hanley - Vacaville CA, US
Monto Kumagai - Davis CA, US
John Lindbo - Vacaville CA, US
David McGee - Vacaville CA, US
Hal Padgett - Vacaville CA, US
Gregory Pogue - Vacaville CA, US

International Classification:

C12Q001/68
A01H005/00

US Classification:

435/006000, 800/278000

Abstract:

The present invention provides methods for rapidly determining the function of nucleic acid sequences by transfecting the same into a host organism to effect expression. Phenotypic and biochemical changes produced thereby are then analyzed to ascertain the function of the nucleic acids which have been transfected into the host organism. The invention also provides methods for silencing endogenous genes by transfecting hosts with nucleic acid sequences to effect expression of the same. The present invention also provides methods for selecting desired functions of RNAs and proteins by the use of virus vectors to express libraries of nucleic acid sequence variants. Moreover, the present invention provides methods for inhibiting an endogenous protease of a plant host.

US Patent:

20030027182, Feb 6, 2003

Filed:

Mar 21, 2002

Appl. No.:

10/105697

Inventors:

Monto Kumagai - Davis CA, US
Guy Della-Cioppa - Vacaville CA, US
Robert Erwin - Vacaville CA, US
David McGee - Vacaville CA, US

International Classification:

C12Q001/68
A01H001/00

US Classification:

435/006000, 800/288000

Abstract:

The present invention provides a method of compiling a plant functional gene profile, a method of changing the phenotype or biochemistry of a plant, a method of determining a change in phenotype or biochemistry of a plant, and a method of determining the presence of a trait in plant. The methods comprise expressing transiently a nucleic acid sequence of a plant into a host plant to affect phenotypic or biochemical changes in the host plant. A viral vector functional genomic screen has been developed to identify nucleotide sequences in transfected plants by systemically knocking out endogenous gene expression in an antisense mechanism. Once the presence of a trait in a plant is identified by phenotypic or biochemical changes in the host plant, the nucleic acid insert in the cDNA clone or in the vector that results in the changes is then sequenced. The present invention exemplifies that genes encoding GTP binding proteins in one plant can silence endogenous gene expression in a different plant.

US Patent:

20030027183, Feb 6, 2003

Filed:

Apr 10, 2002

Appl. No.:

10/120630

Inventors:

Monto Kumagai - Davis CA, US
Guy della-Cioppa - Vacaville CA, US
Robert Erwin - Vacaville CA, US
David McGee - Vacaville CA, US

International Classification:

C12Q001/68
A01H001/00

US Classification:

435/006000, 800/278000, 800/280000

Abstract:

The present invention provides a method of compiling a plant functional gene profile, a method of changing the phenotype or biochemistry of a plant, a method of determining a change in phenotype or biochemistry of a plant, and a method of determining the presence of a trait in plant. The methods comprise expressing transiently a nucleic acid sequence of a plant into a host plant to affect phenotypic or biochemical changes in the host plant. A viral vector functional genomic screen has been developed to identify nucleotide sequences in transfected plants by systemically knocking out endogenous gene expression in an antisense mechanism. Once the presence of a trait in a plant is identified by phenotypic or biochemical changes in the host plant, the nucleic acid insert in the cDNA clone or in the vector that results in the changes is then sequenced. The present invention exemplifies that genes encoding GTP binding proteins in one plant can silence endogenous gene expression in a different plant.

US Patent:

20030028926, Feb 6, 2003

Filed:

May 1, 2002

Appl. No.:

10/137765

Inventors:

Monto Kumagai - Davis CA, US
Guy della-Cioppa - Vacaville CA, US
Robert Erwin - Vacaville CA, US
David McGee - Vacaville CA, US

International Classification:

C12Q001/68
C12N015/87

US Classification:

800/288000, 435/006000

Abstract:

The present invention provides a method of compiling a positive sense functional gene profile of an organism, a method of changing the phenotype or biochemistry of an organism, a method of determining a change in phenotype or biochemistry of an organism, and a method of determining the presence of a trait in an organism, and a method of isolating human cDNA. The methods comprise expressing transiently a nucleic acid sequence of a donor organism into a host plant to affect phenotypic or biochemical changes in the host organism. A viral vector functional genomic screen has been developed to identify nucleotide sequences in transfected plants by enhancing or supressing an endogenous gene expression in a positive sense mechanism, or by overexpressing a new protein. Once the presence of a trait in a plant is identified by phenotypic or biochemical changes in the host plant, the nucleic acid insert in the cDNA clone or in the vector that results in the changes is then sequenced. The present invention provides a method for discovering new gene and its function in a donor organism such as human by transfecting a nucleic acid sequence of the donor organism into a host organism in a positive sense.

US Patent:

20030041355, Feb 27, 2003

Filed:

May 14, 2002

Appl. No.:

10/146337

Inventors:

Monto Kumagai - Davis CA, US
Guy della-Cioppa - Vacaville CA, US
Robert Erwin - Vacaville CA, US
David McGee - Vacaville CA, US

International Classification:

C12Q001/68
A01H001/00

US Classification:

800/288000, 435/006000

Abstract:

The present invention provides a method of compiling a positive sense functional gene profile of an organism, a method of changing the phenotype or biochemistry of an organism, a method of determining the presence of a trait in an organism, and a method of humanizing a plant cDNA. The methods comprise expressing transiently a nucleic acid sequence of a donor organism into a host plant to affect phenotypic or biochemical changes in the host organism. Once the presence of a trait in a plant is identified by phenotypic or biochemical changes in the host plant, the nucleic acid insert in the cDNA clone or in the vector that results in the changes is then sequenced. The present invention provides a method for discovering new gene and its function in a donor organism such as human by transfecting a nucleic acid sequence of the donor organism into a host organism in a positive sense.

US Patent:

20030064392, Apr 3, 2003

Filed:

May 22, 2002

Appl. No.:

10/154671

Inventors:

Monto Kumagai - Davis CA, US
Guy della-Cioppa - Vacaville CA, US
Robert Erwin - Vacaville CA, US
David McGee - Vacaville CA, US

International Classification:

C12Q001/68
A01H001/00
A01H005/00

US Classification:

435/006000, 800/288000

Abstract:

The present invention provides a method of compiling a functional gene profile of an organism, a method of changing the phenotype or biochemistry of a plant, a method of determining a change in phenotype or biochemistry of a plant, a method of determining the presence of a trait in plant, and a method of humanizing plant cDNA. The methods comprise expressing transiently a nucleic acid sequence of a non-plant donor organism into a host plant by a viral vector to affect phenotypic or biochemical changes in the host plant. The present invention provides a method for discovering the presence of a new gene and determining its function and sequence in a donor organism such as human by transfecting a nucleic acid sequence of the donor organism into a host plant to knock out the endogenous gene expression.