David Alan Nielsen

US Patent:

20140057325, Feb 27, 2014

Filed:

Mar 8, 2012

Appl. No.:

14/003713

Inventors:

David Ross Nielsen - Tempe AZ, US
Rebekah McKenna - Mesa AZ, US

Assignee:

Arizona Board of Regents, a body corporate of the State of Arizona, acting for and on behalf of - Scottsdale AZ

International Classification:

C12P 5/00
C12P 17/02

US Classification:

435123, 435166

Abstract:

A method for the in vivo production of styrene from renewable substrates using a recombinant microorganism is disclosed. Additionally, a method for the in vivo production of styrene oxide from renewable substrates using a recombinant microorganism is also disclosed. In both cases, the host cell expresses at least one gene encoding a polypeptide that possesses phenylalanine ammonia lyase activity in addition to at least one gene encoding a polypeptide that possesses trans-cinnamic acid decarboxylase activity. In the case of styrene oxide, the host cell must additionally express at least one gene encoding a polypeptide that possesses styrene monooxygenase activity.

US Patent:

20200385761, Dec 10, 2020

Filed:

Jun 5, 2020

Appl. No.:

16/894416

Inventors:

David Nielsen - Tempe AZ, US
Christopher Jones - Tempe AZ, US

Assignee:

Arizona Board of Regents on Behalf of Arizona State University - Tempe AZ

International Classification:

C12N 15/90
C12Q 1/6874
C12N 15/86
C12Q 1/6897

Abstract:

Compositions and methods for genetically modifying at least one nucleic acid sequence of interest in a polyploid organism.

US Patent:

20180312884, Nov 1, 2018

Filed:

Mar 28, 2018

Appl. No.:

15/938742

Inventors:

- Scottsdale AZ, US
Weiwen Zhang - Chandler AZ, US
Deirdre Meldrum - Phoenix AZ, US
David Nielsen - Tempe AZ, US

International Classification:

C12P 7/52
C07C 59/01
C12N 9/04
C12N 9/16
C12N 9/10

Abstract:

Construction and expression of synthetic pathways to produce (S) or (R)-3-hydroxybutyrate (3HB) as enantiomerically-pure products by genetically engineering cyanobacterium sp. PCC 6803. Under optimized growth conditions, the pathway employing phaA and phaB from was the most effective, producing up to 533.45.5 mg/l (R)-3HB after 21 days photosynthetic cultivation. For the first time, the feasibility and high efficiency of producing 3HB using solar energy and COas sole energy and carbon sources by engineered cyanobacteria is demonstrated.

US Patent:

20160230194, Aug 11, 2016

Filed:

Aug 13, 2014

Appl. No.:

15/022540

Inventors:

David NIELSEN - Tempe AZ, US
Shawn PUGH - Mesa AZ, US
Rebekah MCKENNA - Mesa AZ, US

Assignee:

Arizona Board of Regents on behalf of Arizona State University - Scottsdale AZ

International Classification:

C12P 5/02
C12N 9/88

Abstract:

A method for the in vivo production of 1,3-butadiene from 2,4-pentadienoate is described (FIG. ). Enzymes capable of decarboxylating 2,4-pentadienoate to 1,3-butadiene have been discovered. Recombinant expression of these newly discovered enzymes has resulted in the engineering of microorganisms capable of producing 1,3-butadiene when cultured in the presence of 2,4-pentadienoate. 1,3-butadienoate is an important monomer used in the manufacturing of rubbers and plastics. This invention will help to enable the biological production of 1,3-butadiene from renewable resources such as sugar, for example.

US Patent:

20160160244, Jun 9, 2016

Filed:

Aug 12, 2014

Appl. No.:

14/908967

Inventors:

David NIELSEN - Tempe AZ, US
Shawn PUGH - Mesa AZ, US
Brian THOMPSON - Tempe AZ, US

Assignee:

Arizona Board of Regents on behalf of Arizona Stat e University - Scottsdale AZ

International Classification:

C12P 7/22
C12P 7/44

Abstract:

Novel methods for the in vivo production of phenol from renewable substrates using a recombinant microorganism (FIG. ). Additionally, methods for the in vivo production of catechol and cis,cis-muconic acid from renewable substrates using a recombinant microorganism are disclosed. A host cell expresses at least one gene encoding a polypeptide that possesses isochorismate synthase activity, at least one gene encoding a polypeptide that possesses isochorismate pyruvate lyase activity, and at least one gene encoding a polypeptide that possesses salicylic acid decarboxylase activity. In the case of catechol, the host cell must additionally express at least one gene encoding a polypeptide that possesses phenol 2-monooxy-genase activity. In the case of cis,cis-muconic acid, the host cell must additionally express at least one gene encoding a polypeptide that possesses phenol 2-monooxygenase activity and at least one gene encoding a polypeptide that possesses catechol-1,2-dioxygenase activity.