William J. Lacey - North Andover MA, US Gregory Mathus - Concord MA, US David M. Root - Westford MA, US John A. Ryan - Clinton MA, US
Assignee:
Corning Incorporated - Corning NY
International Classification:
C12M001/12
US Classification:
4352975, 4353052, 422101, 422102
Abstract:
A high-throughput cell or tissue culture apparatus, which is configurable to an industry-standard well plate format, is provided. The apparatus comprises a number of vessels, which may be suspended in wells of a plate. Each vessel has at least one sidewall defining a first opening and a second opening, each of predetermined cross-sectional area. The second opening has an inner cross-sectional area greater than either the inner cross-sectional area of the first opening or a cross-sectional area in a horizontal plane between the first and second openings. A relatively large substrate area is provided in each vessel for supporting tissue cultures in a fluid medium.
Detection Of Reactions And Metabolic Changes With Fluorscent Materials
Valerie Lemee - Sausheim, FR Pascal Marque - Veneux-Ies-Sablons, FR Marylene Pecheul - Souppes Sur Loing, FR David Root - Westford MA, US
International Classification:
G01N021/64
US Classification:
422/057000, 436/172000
Abstract:
A system, method and device for the detection of reactions between analytes, (e.g., DNA, biomolecules, or cells) and a second compound are disclosed. The present invention includes a coating of a fluorescent material having a fluorescence that changes with temperature. The fluorescent material is associated with a substrate, and can be used for any type of surface reaction that requires determination of temperature conditions at an interface between the surface and the reaction analyte subject to assay. The substrate may be, for example, a microarray chip or microplate, preferably suitable for use in high-throughput screening of biomolecules or cells. Substrates containing the fluorescent material also can be used to compensate for temperature variations in refractive index in optical sensors.
Sma Therapy And Cell Based Assay For Identifying Therapies
Brent Stockwell - Boston MA, US David Root - Brookline MA, US
International Classification:
A61K031/404
US Classification:
514/418000
Abstract:
This invention relates to therapies for diseases involving splicing defects, such as spinal muscular atrophy (SMA), and methods to identify compounds for treating this disease. The invention specifically provides for therapies comprised of small molecule compounds identified by cell-based high-throughput screening assays. These assays utilize engineered splicing constructs that fuse pre-mRNA fragments to a reporter gene. The fragments contain exons and at least one intron of a gene mutated in such a way to cause disease. Additionally, the invention provides for methods to monitor the effects of drugs on splicing and gene expression in vivo, in transgenic animals.
Methods For Providing High-Integrity Enrollments Into Biometric Authentication Databases
Without control over the point of enrollment into biometric authentication databases, fraudulent enrollment is an expected consequence. Such enrollment fraud would minimize the potential benefits derived from the superior authentication capabilities offered, in varying degrees, by different biometric technologies. In a world where identity theft and fraud is rising along with the consequences of said behavior, a better enrollment system is needed. This present invention is intended to control the point of enrollment into biometric authentication databases, limiting said enrollments to only those identity/biometric data pairings that have been certified by this invention (process) to some level of identity-integrity. This present invention is further intended to allow a greater level of confidence in the identity-integrity of transactions authenticated with a higher level of certified trust than is available through other authentication methods, or even through biometric authentication provided by this invention at lower certified levels of trust.
Biao Luo - Lexington MA, US David Root - Brookline MA, US Xiaoping Yang - Brookline MA, US Gregory Hinkle - Plymouth MA, US
International Classification:
C40B 40/08 C40B 50/06
US Classification:
435006000, 435091200
Abstract:
Described herein is a method of cloning synthetic oligos (including in situ synthesized oligos) into an (one or more) expression vector for library (e.g., shRNA library) production. The oligos are synthesized with one portion of the first stem of the hairpin, followed by a first loop sequence, the complete second stem, a second loop sequence, and finished with the remaining portion of the first stem of the hairpin. The two portions of the first stem anneal to the second stem, juxtaposing the 5′ end close to the 3′ end of the oligo. The methods described herein selected for hairpins with perfectly base-paired stems. After annealing, a ligase is added to the annealed oligos and the base-paired hairpins are preferentially annealed, and ligated, creating closed circular oligos. The now circularized hairpins served as templates for rolling circle amplification using a polymerase with high processivity. One or more primers complementary to the two strands of the amplified double stranded circular hairpins initiate the rolling circle amplification in the presence of a polymerase. Using primers (e.g., a sense and antisense primer), the rolling circle amplification yields double stranded hairpin sequences. These can be digested (e.g., using restriction enzymes) to produce a double-stranded hairpin fragment encoding a single hairpin. The fragment can be cloned into an appropriately digested vector for a variety of uses including expression.
Substrates For Isolating, Reacting And Microscopically Analyzing Materials
Jean I. Montagu - Brookline MA, US Roger Dowd - Natick MA, US David Root - Chelmsford MA, US
International Classification:
B01J 19/00
US Classification:
422102
Abstract:
An immobilizing device for biological material comprises a rigid support () carrying a substrate layer (′) of polymer having biological immobilizing properties, e.g. for amino and nucleic acids. Substantially solid ultra-thin substrate layers (′) having a thickness less than about 5 micron, preferably between about 0.1 and 0.5 micron, and micro-porous, ultra-thin substrate layers (′) having a thickness less than about 5 micron, preferably less than 3 micron, 2 or 1 micron are shown, which may be segmented by isolating moats M. The substrate layer is on a microscope slide (), round disc (), bio-cassette, at the bottom of a well of a multiwell plate, and as a coating inside a tube. Fluorescence or luminescence intensity and geometric calibration spots () are shown. Reading is enhanced by the intensity calibration spots () to enable normalization of readings under uneven illumination conditions, as when reading by dark field, side illumination mode. The reference spots are shown being printed simultaneously with printing an array of biological spots or with the same equipment. Methods of forming layers of the device include controlled drawing from a bath of coating composition and drying, and spinning of C-D shaped substrates. Post-forming treatment is shown by corona treatment and radiation. Adherent metal oxides (), silica-based materials and other materials are used to unite layers of the composite. In multiwell plates the oxide promotes joining of a bottom plate (′) and upper, well-defining structure () of dissimilar material. The oxides () also provide beneficial opacity to prevent light entering the glass support, for applying potential to the substrate, etc.
This invention relates to therapies for diseases involving splicing defects, such as spinal muscular atrophy (SMA), and methods to identify compounds for treating this disease. The invention specifically provides for therapies comprised of small molecule compounds identified by cell-based high-throughput screening assays. These assays utilize engineered splicing constructs that fuse pre-mRNA fragments to a reporter gene. The fragments contain exons and at least one intron of a gene mutated in such a way to cause disease. Additionally, the invention provides for methods to monitor the effects of drugs on splicing and gene expression in vivo, in transgenic animals.
Substrates For Isolating, Reacting And Microscopically Analyzing Materials
An immobilizing device for biological material comprises a rigid support () carrying a substrate layer (′) of polymer having biological immobilizing properties, e.g. for amino and nucleic acids. Substantially solid ultra-thin substrate layers (′) having a thickness less than about 5 micron, preferably between about 0.1 and 0.5 micron, and microporous, ultra-thin substrate layers (′) having a thickness less than about 5 micron, preferably less than 3 micron, 2 or 1 micron are shown, which may be segmented by isolating moats M. The substrate layer is on a microscope slide (), round disc (), bio-cassette, at the bottom of a well of a multiwell plate, and as a coating inside a tube. Fluorescence or luminescence intensity and geometric calibration spots () are shown. Reading is enhanced by the intensity calibration spots () to enable normalization of readings under uneven illumination conditions, as when reading by dark field, side illumination mode. The reference spots are shown being printed simultaneously with printing an array of biological spots or with the same equipment. Methods of forming layers of the device include controlled drawing from a bath of coating composition and drying, and spinning of C-D shaped substrates. Post-forming treatment is shown by corona treatment and radiation. Adherent metal oxides (), silica-based materials and other materials are used to unite layers of the composite. In multiwell plates the oxide promotes joining of a bottom plate (′) and upper, well-defining structure () of dissimilar material. The oxides () also provide beneficial opacity to prevent light entering the glass support, for applying potential to the substrate, etc.
Roots Siding Inc Macedon, NY Jan 2012 to Jan 2014 OwnerExterior Specialists Inc. Palmyra, NY Jan 2009 to Jan 2012 ForemanExterior Specialists Inc. Palmyra, NY Jan 2006 to Jan 2009 Laborer/ InstallerMobil on the Run Palmyra, NY Jan 2005 to Jan 2006 Clerk/Cashier
Education:
Finger Lakes Community College Canandaigua, NY 2005 to 2007 Business AdministrationPalmyra-Macedon High School Palmyra, NJ 2002 to 2005 Regents Diploma
Sacramento Occupational Medical Group 1550 Hbr Blvd STE 110, West Sacramento, CA 95691 9163729893 (phone), 9163720630 (fax)
Sacramento Occupational Medical Group 5665 Power Inn Rd STE C120, Sacramento, CA 95824 9163876929 (phone), 9163876977 (fax)
Education:
Medical School Wake Forest University School of Medicine Graduated: 1962
Languages:
English Spanish
Description:
Dr. Root graduated from the Wake Forest University School of Medicine in 1962. He works in Sacramento, CA and 1 other location and specializes in Occupational Medicine.
Palo Alto, CA New York City, NY Houston, TX Orlando, FL Vail, CO Las Vegas, NV Greenwich, CT Boise, ID Santa Fe, NM
Work:
Scratch Restaurant - Chef
Education:
CIA
Relationship:
Married
About:
Master of kitchen evolution, advocate of farm to table, follower of Kenpo, love to put tires in the dirt, husband of an amazing woman, traveler of roads less explored, gypsy at heart, interested citiz...
David Root
Work:
Cases2Go - President (1982) Professional Packing & Crating - Owner (1983-2000)
Education:
University of South Florida - MBA, Business Administration
About:
Founder and owner of Root International, Inc. dba Cases2Go. My career in the packaging industry began in 1982 with the founding of Professional Packaging & Crating, providing export and military ...
David Root
About:
RootRocks is a unique solo music act boasting a full live rock and blues band sound. Featuring popular music from the Fifties, Sixties, Seventies and Eighties, RootRocks live guitar and vocals perfor...
Tagline:
RootRocks - Full live rock 'n roll band sound in a solo act
David Root
David Root
David Root
David Root
David Root
Youtube
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