David Wells Snyder

US Patent:

6471962, Oct 29, 2002

Filed:

Oct 2, 1992

Appl. No.:

07/956473

Inventors:

David Snyder - Bowie MD

Assignee:

The University of Maryland - College Park MD

International Classification:

A61K 3942

US Classification:

4241471, 435 7021, 435339, 5303883

Abstract:

Two monoclonal antibodies which neutralize all infectious bursal disease viruses are employed in the preparation of infectious bursal disease vaccines. A panel of monoclonal antibodies can be used to characterize the infectious bursal disease virus strains present, according to neutralization site characteristics.

US Patent:

61563145, Dec 5, 2000

Filed:

Jun 15, 1999

Appl. No.:

9/332978

Inventors:

Vikram Vakharia - Bowie MD
David B. Snyder - Bowie MD

Assignee:

The University of Maryland College Park - College Park MD

International Classification:

A61K 3942
C07H 2104
C07K 1608

US Classification:

4241471

Abstract:

Chimeric cDNA for the expression of immunogenic polypeptides include the genetic epitopic determinants for a base infectious bursal disease virus strain and at least one other infectious bursal disease virus strain. The genetic epitopic determinants encode amino acids or amino acid sequences which define epitopes bound to by previously established monoclonal antibodies. The immunogens expressed by the cDNA may be employed to provide a vaccine against a plurality of IBDV strains. The epitopic determinant of IBDV lethal strains has been detected, and an immunogen for conferring immunity with respect thereto is disclosed. Similarly, a monoclonal antibody specific for IBDV lethal strains is identified, and a vaccine for passive immunization therewith is also disclosed. Immunogens exhibiting conformational epitopes, in the form of virus-like particles, are effective in the preparation of vaccines.

US Patent:

49083058, Mar 13, 1990

Filed:

Oct 21, 1987

Appl. No.:

7/110738

Inventors:

David B. Snyder - Seabrook MD

Assignee:

The University of Maryland - College Park MD

International Classification:

G01N 3353
G01N 33577
C07K 1514
C12N 100

US Classification:

435 5

Abstract:

A method for determining the level of infectious bursal disease virus IBDV neutralizing antibody in poultry comprising a labeled monoclonal antibody R63 having the IBDV neutralizing capability of the monoclonal antibody expressed by hybridoma cell line ATCC HB-9490. Monoclonal antibody R63 is specific to all known IBDV strains and serotypes and competes only with itself and other antibodies recognizing the same neutralizing epitope present in poultry sera. In a competition assay, the increase in unbound labeled monoclonal antibody is an indication of the level of IBDV neutralizing antibody present in poultry sera.

US Patent:

57889708, Aug 4, 1998

Filed:

Mar 29, 1994

Appl. No.:

8/219262

Inventors:

Vikram Vakharia - Bowie MD
David B. Snyder - Bowie MD

Assignee:

The University of Maryland College Park - College Park MD

International Classification:

A61K 39295
C12N 1540
C07K 1408

US Classification:

4241921

Abstract:

Chimeric cDNA for the expression of immunogenic polypeptides include the genetic epitopic determinants for a base infectious bursal disease virus strain and at least one other infectious bursal disease virus strain. The genetic epitopic determinants encode amino acids or amino acid sequences which define epitopes bound to by previously established monoclonal antibodies. The immunogens expressed by the cDNA may be employed to provide a vaccine against a plurality of IBDV strains. The epitopic determinant of IBDV lethal strains has been detected, and an immunogen for conferring immunity is disclosed. Similarly, a monoclonal antibody specific for IBDV lethal strains is identified, and a vaccine for passive immunization is also disclosed. Immunogens exhibiting conformational epitopes, in the form of virus-like particles, are effective in the preparation of vaccines.

US Patent:

49564528, Sep 11, 1990

Filed:

Jun 12, 1987

Appl. No.:

7/061083

Inventors:

David B. Snyder - Lanham MD
Warren W. Marquardt - New Carrollton MD
Sammy L. Gorham - Upper Marlboro MD
Dolores P. Lana - Baltimore MD

Assignee:

The University of Maryland - College Park MD

International Classification:

C07K 1514
C07K 1528
C12N 1500
A61K 39395

US Classification:

530387

Abstract:

Monoclonal antibodies effective in preventing infectious bursal disease in chickens, by neutralizing one or more virus strains thereof, have been isolated and obtained from deposited hybridomas. Vaccination of an entire poultry population with a vaccine prepared from these monoclonal antibodies gives a uniform level of protection against all strains of infectious bursal disease tested. The monoclonal antibodies were effective in inducing priming for an active anti-viral response in a heterologous host.

US Patent:

50646460, Nov 12, 1991

Filed:

Aug 2, 1988

Appl. No.:

7/227311

Inventors:

David B. Snyder - Seabrook MD

Assignee:

The University of Maryland - College Park MD

International Classification:

A61K 3912
C12N 700

US Classification:

424 89

Abstract:

A new virus not neutralized or bound by monoclonal antibodies which bind and neutralize all IBD vaccines of current art, and capable of inducing infectious bursal disease in poultry is identified, in essentially pure form. A test kit, and assay for the presence of the virus is disclosed, together with the vaccine incorporating the virus.

US Patent:

56329890, May 27, 1997

Filed:

Sep 15, 1992

Appl. No.:

7/944525

Inventors:

David B. Snyder - Bowie MD
Vikram Vakharia - Bowie MD
Heinrich D. Luetticken - Boxmeer, NL

Assignee:

University of Maryland - College Park MD

International Classification:

A61K 3942
A61K 3912
C12N 704
C12N 518

US Classification:

4241391

Abstract:

Live, attenuated Delaware infectious bursal disease viruses are screened for effectiveness by a monoclonal antibody specific for said Delaware strain. Those which are not bound by the antibody are not or may not be as effective in conferring protection against homologous wild type infectious bursal disease infection. Currently available live, attenuated vaccines do not include a virus having the binding site specified. A monoclonal antibody specific for the Delaware strain also provides a vaccine for passive immunization.

US Patent:

60177594, Jan 25, 2000

Filed:

Feb 27, 1998

Appl. No.:

9/031655

Inventors:

Vikram Vakharia - Bowie MD
David B. Snyder - Bowie MD

Assignee:

University of Maryland College Park - College Park MD

International Classification:

A61K 39295
C12N 1540
C07K 1408

US Classification:

435325

Abstract:

Chimeric cDNA for the expression of immunogenic polypeptides include the genetic epitopic determinants for a base infectious bursal disease virus strain and at least one other infectious bursal disease virus strain. The genetic epitopic determinants encode amino acids or amino acid sequences which define epitopes bound to by previously established monoclonal antibodies. The immunogens expressed by the cDNA may be employed to provide a vaccine against a plurality of IBDV strains. The epitopic determinant of IBDV lethal strains has been detected, and an immunogen for conferring immunity with respect thereto is disclosed. Similarly, a monoclonal antibody specific for IBDV lethal strains is identified, and a vaccine for passive immunization therewith is also disclosed. Immunogens exhibiting conformational epitopes, in the form of virus-like particles, are effective in the preparation of vaccines.