David P Spencer

US Patent:

7632983, Dec 15, 2009

Filed:

Mar 5, 2004

Appl. No.:

10/794615

Inventors:

Lynn F. Dickey - Cary NC, US
John R. Gasdaska - Carrboro NC, US
Kevin M. Cox - Raleigh NC, US
Charles G. Peele - Apex NC, US
David Spencer - Chapel Hill NC, US

Assignee:

Biolex Therapeutics, Inc. - Durham NC

International Classification:

C12N 15/82
A01H 5/00

US Classification:

800288, 435468

Abstract:

Methods, nucleic acid sequences, and transformed duckweed plant or duckweed nodule cultures for the expression and the secretion of biologically active polypeptides from genetically engineered duckweed are provided. Expression of recombinant polypeptides in duckweed is improved by modifying the nucleotide sequence of the expression cassette encoding the polypeptide for improved expression in duckweed. Recovery of biologically active polypeptides from duckweed is improved by linking the biologically active polypeptide to a signal peptide that directs the secretion of the polypeptide into the culture medium. Methods of producing recombinant monoclonal antibodies having effector function, and antibodies produced by these methods are provided.

US Patent:

7659445, Feb 9, 2010

Filed:

Feb 11, 2005

Appl. No.:

11/056621

Inventors:

David Spencer - Chapel Hill NC, US
Lynn F. Dickey - Cary NC, US
John R. Gasdaska - Carrboro NC, US
Xiaowei Wang - Carrboro NC, US
Kevin M. Cox - Raleigh NC, US
Charles G. Peele - Apex NC, US

Assignee:

Biolex Therapeutics, Inc. - Pittsboro NC

International Classification:

C12N 15/82
C12N 15/12
C12N 15/62

US Classification:

800288, 435 696, 435 698, 536 241

Abstract:

The present invention provides methods and compositions for the production of recombinant plasminogen, microplasminogen, and fragments thereof in a duckweed expression system. It is the novel finding of the present invention that a duckweed expression system may be used to produce high levels of plasminogen and microplasminogen. The duckweed-produced plasminogen and microplasminogen can be activated to produce a polypeptide having protease activity. Thus, the invention encompasses methods for the expression of plasminogen, microplasminogen, and fragments thereof in duckweed, duckweed plants that are transformed with expression cassettes for the expression of plasminogen, microplasminogen, and fragments thereof, and nucleic acids comprising nucleotide sequences encoding plasminogen, microplasminogen, and fragments thereof, where these nucleotide sequences are modified to enhance their expression in duckweed.

US Patent:

8017836, Sep 13, 2011

Filed:

Dec 22, 2009

Appl. No.:

12/644640

Inventors:

David Spencer - Chapel Hill NC, US
Lynn F. Dickey - Cary NC, US
John R. Gasdaska - Carrboro NC, US
Xiaowei Wang - Carrboro NC, US
Kevin M. Cox - Raleigh NC, US
Charles G. Peele - Apex NC, US

Assignee:

Biolex Therapeutics, Inc. - Pittsboro NC

International Classification:

A01H 5/00
C12N 15/82
C12N 5/04
C12N 5/10
C12N 15/12
C12N 15/62

US Classification:

800288, 435 696, 435 698

Abstract:

The present invention provides methods and compositions for the production of recombinant plasminogen, microplasminogen, and fragments thereof in a duckweed expression system. It is the novel finding of the present invention that a duckweed expression system may be used to produce high levels of plasminogen and microplasminogen. The duckweed-produced plasminogen and microplasminogen can be activated to produce a polypeptide having protease activity. Thus, the invention encompasses methods for the expression of plasminogen, microplasminogen, and fragments thereof in duckweed, duckweed plants that are transformed with expression cassettes for the expression of plasminogen, microplasminogen, and fragments thereof, and nucleic acids comprising nucleotide sequences encoding plasminogen, microplasminogen, and fragments thereof, where these nucleotide sequences are modified to enhance their expression in duckweed.

US Patent:

20220323083, Oct 13, 2022

Filed:

Jan 21, 2022

Appl. No.:

17/581134

Inventors:

- Boerne TX, US
Todd J. KRUMMENACHER - Lakewood CO, US
Jeremy REYNOLDS - Lakewood CO, US
David SPENCER - Boerne TX, US
Luke William FISHER - Lakewood CO, US

International Classification:

A61B 17/12
A61M 25/00
A61M 25/10
A61M 25/02

Abstract:

An occlusion catheter system includes an inflation catheter member and an occlusion balloon. The proximal and distal balloon ends are connected to the inflation catheter between the proximal and distal catheter ends. A distal pressure sensor is attached to the inflation catheter member between the proximal balloon end and the atraumatic tip. An inflatable spine is connected to the inflation catheter. The proximal spine end is connected to the inflation catheter near the proximal balloon end and the distal spine end is connected to the inflation catheter near the distal balloon end. The occlusion balloon and the inflatable spine are configured to define blood flow channels with the internal surface and the external balloon surface when the occlusion catheter system is at least partially positioned in the vessel and the occlusion balloon and the inflatable spine are in a partially inflated configuration.

US Patent:

20190307462, Oct 10, 2019

Filed:

Jun 24, 2019

Appl. No.:

16/450067

Inventors:

- Boerne TX, US
Todd J. KRUMMENACHER - Lakewood CO, US
Jeremy REYNOLDS - Lakewood CO, US
David SPENCER - Boerne TX, US
Luke William FISHER - Lakewood CO, US

International Classification:

A61B 17/12
A61M 25/10
A61M 25/00
A61M 25/02

Abstract:

An occlusion catheter system includes an inflation catheter member and an occlusion balloon. The proximal and distal balloon ends are connected to the inflation catheter between the proximal and distal catheter ends. A distal pressure sensor is attached to the inflation catheter member between the proximal balloon end and the atraumatic tip. An inflatable spine is connected to the inflation catheter. The proximal spine end is connected to the inflation catheter near the proximal balloon end and the distal spine end is connected to the inflation catheter near the distal balloon end. The occlusion balloon and the inflatable spine are configured to define blood flow channels with the internal surface and the external balloon surface when the occlusion catheter system is at least partially positioned in the vessel and the occlusion balloon and the inflatable spine are in a partially inflated configuration.

US Patent:

20190076152, Mar 14, 2019

Filed:

Jun 2, 2017

Appl. No.:

15/573054

Inventors:

- Boerne TX, US
Todd J. KRUMMENACHER - Lakewood CO, US
Jeremy REYNOLDS - Lakewood CO, US
David SPENCER - Boerne TX, US
Luke William FISHER - Lakewood CO, US

Assignee:

Prytime Medical Devices, Inc. - Boerne TX

International Classification:

A61B 17/12
A61M 25/10

Abstract:

An occlusion catheter system includes an inflation catheter member and an occlusion balloon. The proximal and distal balloon ends are connected to the inflation catheter between the proximal and distal catheter ends. A distal pressure sensor is attached to the inflation catheter member between the proximal balloon end and the atraumatic tip. An inflatable spine is connected to the inflation catheter. The proximal spine end is connected to the inflation catheter near the proximal balloon end and the distal spine end is connected to the inflation catheter near the distal balloon end. The occlusion balloon and the inflatable spine are configured to define blood flow channels with the internal surface and the external balloon surface when the occlusion catheter system is at least partially positioned in the vessel and the occlusion balloon and the inflatable spine are in a partially inflated configuration.