David C Sternberg

US Patent:

7476516, Jan 13, 2009

Filed:

Jul 25, 2003

Appl. No.:

10/627124

Inventors:

Maria Tang - Fairfield CA, US
Alan Sloma - Davis CA, US
David Sternberg - Davis CA, US
Regine Behr - Roseville CA, US

Assignee:

Novozymes, Inc. - Davis CA

International Classification:

C12P 21/06

US Classification:

435 691, 435 693, 435 697, 435 711, 435243, 4352521, 4352523, 43525231

Abstract:

The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parent cell under conditions conducive for the production of the heterologous biological substance, wherein (i) the mutant cell comprises a first nucleic acid sequence directing synthesis of the heterologous biological substance and a second nucleic acid sequence comprising a modification of at least one of the genes cypX and yvmC, which are involved in the production of a red pigment, and (ii) the mutant cell is deficient in the production of the red pigment compared to the parent cell when cultivated under the same conditions; and (b) recovering the heterologous biological substance from the cultivation medium. The present invention also relates to mutants of cells and methods for producing the mutants.

US Patent:

7666625, Feb 23, 2010

Filed:

Dec 9, 2008

Appl. No.:

12/331323

Inventors:

Maria Tang - Fairfield CA, US
Alan Sloma - Davis CA, US
David Sternberg - Davis CA, US
Linda Sternberg, legal representative - Davis CA, US
Regine Behr - Roseville CA, US

Assignee:

Novozymes, Inc. - Davis CA

International Classification:

C12P 21/06

US Classification:

435 691, 435 693, 435 697, 435 711, 435243, 4352521, 4352523, 43525231

Abstract:

The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parent cell under conditions conducive for the production of the heterologous biological substance, wherein (i) the mutant cell comprises a first nucleic acid sequence directing synthesis of the heterologous biological substance and a second nucleic acid sequence comprising a modification of at least one of the genes cypX and yvmC, which are involved in the production of a red pigment, and (ii) the mutant cell is deficient in the production of the red pigment compared to the parent cell when cultivated under the same conditions; and (b) recovering the heterologous biological substance from the cultivation medium. The present invention also relates to mutants of cells and methods for producing the mutants.

US Patent:

7811806, Oct 12, 2010

Filed:

Dec 20, 2002

Appl. No.:

10/326185

Inventors:

Alan Sloma - Davis CA, US
Regine Behr - Roseville CA, US
William Widner - Davis CA, US
Maria Tang - Fairfield CA, US
David Sternberg - Davis CA, US
Stephen Brown - Davis CA, US

Assignee:

Novozymes, Inc. - Davis CA

International Classification:

C12N 1/20
C12N 15/74
C12N 15/00
C12P 21/06
C12P 19/04
C12P 19/26

US Classification:

43525231, 435471, 4353201, 435 691, 435101, 435 84

Abstract:

The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a host cell under conditions suitable for production of the hyaluronic acid, wherein the host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene. The present invention also relates to isolated nucleic acid sequences encoding a UDP-glucose 6-dehydrogenase, UDP-glucose pyrophosphorylase, and UDP-N-acetylglucosamine pyrophosphorylase.

US Patent:

8093036, Jan 10, 2012

Filed:

Sep 27, 2010

Appl. No.:

12/891548

Inventors:

Alan Sloma - Davis CA, US
Leslie Naggiar, legal representative - Suffern NY, US
Regine Behr - Roseville CA, US
William Widner - Davis CA, US
Maria Tang - Fairfield CA, US
David Sternberg - Davis CA, US
Linda Sternberg, legal representative - Davis CA, US
Stephen Brown - Davis CA, US

Assignee:

Novozymes, Inc. - Davis CA

International Classification:

C12N 1/20
C12N 15/74
C12N 15/00
C12P 21/06
C12P 19/04
C12P 19/36
C12P 1/00
C12N 9/00
C12N 9/24
C12N 9/88
C07H 21/04

US Classification:

43525231, 435471, 4353201, 435 691, 435101, 435 84, 435 41, 435183, 435200, 435232, 4352523, 536 232, 536 231

Abstract:

The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a host cell under conditions suitable for production of the hyaluronic acid, wherein the host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene. The present invention also relates to isolated nucleic acid sequences encoding a UDP-glucose 6-dehydrogenase, UDP-glucose pyrophosphorylase, and UDP-N-acetylglucosamine pyrophosphorylase.

US Patent:

8124376, Feb 28, 2012

Filed:

Feb 10, 2010

Appl. No.:

12/703550

Inventors:

Maria Tang - Fairfield CA, US
Alan Sloma - Davis CA, US
Leslie Naggiar, legal representative - Suffern NY, US
David Sternberg - Davis CA, US
Linda Sternberg, legal representative - Davis CA, US
Regine Behr - Roseville CA, US

Assignee:

Novozymes, Inc. - Davis CA

International Classification:

C12P 21/06

US Classification:

435 691, 435243, 4352521, 4352523, 43525231, 435 693, 435 697, 435 711

Abstract:

The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parent cell under conditions conducive for the production of the heterologous biological substance, wherein (i) the mutant cell comprises a first nucleic acid sequence directing synthesis of the heterologous biological substance and a second nucleic acid sequence comprising a modification of at least one of the genes cypX and yvmC, which are involved in the production of a red pigment, and (ii) the mutant cell is deficient in the production of the red pigment compared to the parent cell when cultivated under the same conditions; and (b) recovering the heterologous biological substance from the cultivation medium. The present invention also relates to mutants of cells and methods for producing the mutants.

US Patent:

8137951, Mar 20, 2012

Filed:

Apr 11, 2011

Appl. No.:

13/084230

Inventors:

Alan Sloma - Davis CA, US
Leslie Naggiar, legal representative - Suffern NY, US
Regine Behr - Roseville CA, US
William Widner - Davis CA, US
Maria Tang - Fairfield CA, US
David Sternberg - Davis CA, US
Linda Sternberg, legal representative - Davis CA, US
Stephen Brown - Davis CA, US

Assignee:

Novozymes, Inc. - Davis CA

International Classification:

C12N 1/20
C12N 15/74
C12N 15/00
C12P 21/00
C12P 9/26
C12P 1/00
C12N 9/00
C12N 9/24
C12N 9/88
C07H 21/04

US Classification:

43525231, 4352523, 435471, 4353201, 435 691, 435101, 435 84, 435 41, 435183, 435200, 435232, 536 232, 536 237

Abstract:

The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a host cell under conditions suitable for production of the hyaluronic acid, wherein the host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene. The present invention also relates to isolated nucleic acid sequences encoding a UDP-glucose 6-dehydrogenase, UDP-glucose pyrophosphorylase, and UDP-N-acetylglucosamine pyrophosphorylase.

US Patent:

20120149067, Jun 14, 2012

Filed:

Feb 21, 2012

Appl. No.:

13/401663

Inventors:

Alan Sloma - Davis CA, US
Leslie Naggiar - Suffern NY, US
Regine Behr - Roseville CA, US
William Widner - Davis CA, US
Maria Tang - Fairfield CA, US
David Sternberg - Davis CA, US
Linda Sternberg - Davis CA, US
Stephen Brown - Davis CA, US

Assignee:

Novozymes, Inc. - Davis CA

International Classification:

C12P 19/28

US Classification:

435 85

Abstract:

The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a host cell under conditions suitable for production of the hyaluronic acid, wherein the host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene. The present invention also relates to isolated nucleic acid sequences encoding a UDP-glucose 6-dehydrogenase, UDP-glucose pyrophosphorylase, and UDP-N-acetylglucosamine pyrophosphorylase

US Patent:

20140038235, Feb 6, 2014

Filed:

Oct 10, 2013

Appl. No.:

14/050866

Inventors:

- Bagsvaerd, DK
Regine Behr - Roseville CA, US
William Widner - Davis CA, US
Stephen Brown - Davis CA, US
Maria Tang - Fairfield CA, US
David Sternberg - Davis CA, US

Assignee:

Novozymes Biopharma DK A/S - Bagsvaerd

International Classification:

C12P 19/26

US Classification:

435 84, 43525231

Abstract:

The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a host cell under conditions suitable for production of the hyaluronic acid, wherein the host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene. The present invention also relates to isolated nucleic acid sequences encoding a UDP-glucose 6-dehydrogenase, UDP-glucose pyrophosphorylase, and UDP-N-acetylglucosamine pyrophosphorylase