Deborah L Anderson

US Patent:

20030032022, Feb 13, 2003

Filed:

Sep 12, 2001

Appl. No.:

09/950935

Inventors:

Joseph Hill - Brookline MA, US
Zhigang Wang - West Roxbury MA, US
Deborah Anderson - Brookline MA, US
Edmond Yunis - Concord MA, US

Assignee:

The Brigham and Women's Hospital, Inc. - Boston MA

International Classification:

C12Q001/68
G01N033/53

US Classification:

435/006000, 436/510000

Abstract:

The discovery of an association between two variants of the human interleukin-1 gene (IL1B) beta promoter region and a variant of the CD46 gene intron 1 and T-helper type 1 immunity in unexplained recurrent pregnancy loss (URPL) is disclosed. These two IL1B variants are characterized by a base C at position-511 (IL1B-511C) and a base T at position-31 (IL1B-31T) from the transcriptional start site of the IL1B gene. The CD46 gene intron 1 variant is characterized by a change in the HindIII site in this intron. These IL1B promoter variants and CD46 gene intron 1 variants, and reagents for detecting said variants, are useful as diagnostic markers for the diagnosis and management of recurrent pregnancy loss. Accordingly, the invention provides methods and compositions which identify these variants for determining a subject's propensity for having reproductive failure and, particularly, reproductive failure attributed to Th1 cytokines.

US Patent:

54749271, Dec 12, 1995

Filed:

Oct 19, 1993

Appl. No.:

8/137820

Inventors:

Deborah J. Anderson - Brookline MA
Peter M. Johnson - Merseyside, GB2
Richard M. Jack - Brighton MA

Assignee:

Brigham and Women's Hospital - Boston MA

International Classification:

G01N 33554

US Classification:

435 721

Abstract:

Methods for detecting and isolating acrosome-reacted sperm and complement receptor-bearing oocytes using the complement component C3, fragments, or variants thereof, antibodies to a complement receptor, or antibodies to C3, are disclosed. These methods have application in the assessment of fertility, in the preparation of sperm or oocytes for in vitro fertilization or for gamete intrafallopian tube transfer, in promoting or inhibiting fertilization in vitro and in vivo, and in diagnosing and treating infertility.

US Patent:

56655568, Sep 9, 1997

Filed:

May 15, 1995

Appl. No.:

8/441067

Inventors:

Deborah J. Anderson - Brookline MA
Peter M. Johnson - West Kirby, GB2
Richard M. Jack - Brighton MA

Assignee:

Brigham and Women's Hospital - Boston MA

International Classification:

G01N 3353

US Classification:

435 721

Abstract:

Methods for detecting and isolating acrosome-reacted sperm and complement receptor-bearing oocytes using the complement component C3, fragments, or variants thereof, antibodies to a complement receptor, or antibodies to C3, are disclosed. These methods have application in the assessment of fertility, in the preparation of sperm or oocytes for in vitro fertilization or for gamete intrafallopian tube transfer, in promoting or inhibiting fertilization in vitro and in vivo, and in diagnosing and treating infertility.