Deborah Ann Hawkins

US Patent:

6822141, Nov 23, 2004

Filed:

Apr 15, 2002

Appl. No.:

10/121857

Inventors:

Kathryn Dennis Lardizabal - Woodland CA
Gregory A. Thompson - Clarkston WA
Deborah Hawkins - Davis CA

Assignee:

Calgene LLC - Davis CA

International Classification:

A01H 500

US Classification:

800281, 800288, 800298, 536 231, 536 232, 536 236, 536 237, 435419

Abstract:

The invention provides diacylglycerol acyltransferase (DAGAT) proteins, wherein said proteins are active in the formation of triacylglycerol from fatty acyl and diacylglycerol substrates. In one aspect, DAGAT proteins have been isolated and have molecular weights of between approximately 36 and 37 kDa as measured by SDS-PAGE. The invention also provides novel DAGAT polynucleotide and polypeptide sequences and to methods of producing such polypeptides using recombinant techniques. In addition, methods are provided for using such sequences to alter triacylglycerol levels in plants and to treat diseases associated with altered DAGAT activity or expression.

US Patent:

7135617, Nov 14, 2006

Filed:

Jul 31, 2002

Appl. No.:

10/208018

Inventors:

Kathryn Dennis Lardizabal - Woodland CA, US
Gregory A. Thompson - Clarkston WA, US
Deborah Hawkins - Davis CA, US

Assignee:

Calgene LLC - Davis CA

International Classification:

C12N 15/31
C12N 15/54
C12N 15/82
A01H 5/00

US Classification:

800281, 536 231, 536 232, 536 237, 800278, 800288, 800298, 435419, 4353201

Abstract:

The invention provides diacylglycerol acyltransferase (DAGAT) proteins, wherein said proteins are active in the formation of triacylglycerol from fatty acyl and diacylglycerol substrates. In one aspect, DAGAT proteins have been isolated and have molecular weights of between approximately 36 and 37 kDa as measured by SDS-PAGE. The invention also provides novel DAGAT polynucleotide and polypeptide sequences and to methods of producing such polypeptides using recombinannt techniques. In addition, methods are provided for using such sequences to alter triacylglycerol levels in plants and to treat diseases associated with altered DAGAT activity or expression.

US Patent:

7741532, Jun 22, 2010

Filed:

Jul 28, 2008

Appl. No.:

12/181149

Inventors:

Kathryn Dennis Lardizabal - Woodland CA, US
Gregory A. Thompson - Clarkston WA, US
Deborah Hawkins - Davis CA, US

Assignee:

Monsanto Technology LLC - St. Louis MO

International Classification:

C12N 15/31
C12N 15/52
C12N 15/82
A01H 5/00

US Classification:

800281, 800288, 800312, 800314, 8003201, 800322, 435183, 4353201, 435419, 536 231, 536 232, 536 237

Abstract:

The invention provides diacylglycerol acyltransferase (DAGAT) proteins, wherein said proteins are active in the formation of triacylglycerol from fatty acyl and diacylglycerol substrates. In one aspect, DAGAT proteins have been isolated and have molecular weights of between approximately 36 and 37 kDa as measured by SDS-PAGE. The invention also provides novel DAGAT polynucleotide and polypeptide sequences and to methods of producing such polypeptides using recombinant techniques. In addition, methods are provided for using such sequences to alter triacylglycerol levels in plants and to treat diseases associated with altered DAGAT activity or expression.

US Patent:

20040221335, Nov 4, 2004

Filed:

Aug 12, 2003

Appl. No.:

10/604708

Inventors:

Christine Shewmaker - Woodland CA, US
Alison Van Eenennaam - Davis CA, US
Deborah Hawkins - Davis CA, US
Rick Sanders - Davis CA, US

Assignee:

MONSANTO TECHNOLOGY LLC - St. Louis MO

International Classification:

A01H001/00

US Classification:

800/281000

Abstract:

The present invention is in the field of plant genetics and biochemistry. More specifically, the present invention relates to genes affecting the level and composition of oil in plants. In particular, the present invention is directed to methods for increasing the oil level in plants and seeds. Moreover, the present invention includes and provides methods for producing plants and obtaining seeds with altered fatty acid composition.

US Patent:

58248587, Oct 20, 1998

Filed:

Jun 6, 1994

Appl. No.:

8/254404

Inventors:

Huw Maelor Davies - Davis CA
Deborah Hawkins - Davis CA
Janet Nelsen - Davis CA

Assignee:

Calgene, Inc. - Davis CA

International Classification:

A01H 500
C12N 504
C12N 1582

US Classification:

800205

Abstract:

This invention relates to plant LPAATs, means to identify such proteins, amino acid and nucleic acid sequences associated with such protein, and methods to obtain, make and/or use such plant LPAATs. Purification, especially the removal of plant membranes and the substantial separation away from other plant proteins, and use of the plant LPAAT is provided, including the use of the protein as a tool in gene isolation for biotechnological applications. In addition, nucleic acid sequences encoding LPAAT protein regions are provided, and uses of such sequences for isolation of LPAAT genes from plants and for modification of plant triglyceride compositions are considered.

US Patent:

59106301, Jun 8, 1999

Filed:

Oct 21, 1994

Appl. No.:

8/327451

Inventors:

Huw Maelor Davies - Davis CA
Deborah Hawkins - Davis CA
Janet Nelsen - Davis CA
Michael Lassner - Davis CA

International Classification:

A01H 500
C12N 1582

US Classification:

800295

Abstract:

This invention relates to plant LPAATs, means to identify such proteins, amino acid and nucleic acid sequences associated with such protein, and methods to obtain, make and/or use such plant LPAATs. Purification, especially the removal of plant membranes and the substantial separation away from other plant proteins, and use of the plant LPAAT is provided, including the use of the protein as a tool in gene isolation for biotechnological applications. In addition, nucleic acid sequences encoding LPAAT protein regions are provided, and uses of such sequences for isolation of LPAAT genes from plants and for modification of plant triglyceride compositions are considered.

US Patent:

57237611, Mar 3, 1998

Filed:

Jun 2, 1995

Appl. No.:

8/464523

Inventors:

Toni A. Voelker - Davis CA
Ling Yuan - Vacaville CA
Jean Kridl - Davis CA
Deborah Hawkins - Davis CA
Aubrey Jones - Woodland CA

Assignee:

Calgene, Inc. - Davis CA

International Classification:

A01H 500
A01H 1510
C12N 514
C12N 1529
C12N 1552
C12N 1582

US Classification:

800205

Abstract:

This invention relates to plant thioesterases, specifically plant acyl-ACP thioesterases having substantial activity on palmitoyl-ACP substrates. DNA constructs useful for the expression of a plant palmitoyl-ACP thioesterase in a plant seed cell are described. Such constructs will contain a DNA sequence encoding the plant palmitoyl-ACP thioesterase of interest under the control of regulatory elements capable of preferentially directing the expression of the plant palmitoyl-ACP thioesterase in seed tissue, as compared with other plant tissues, when such a construct is expressed in a transgenic plant. This invention also relates to methods of using a DNA sequence encoding a plant palmitoyl-ACP thioesterase for the modification of the proportion of free fatty acids produced in a plant seed cell. Plant palmitoyl-ACP thioesterase sequences exemplified herein include Cuphea, leek, mango and elm. Transgenic plants having increased levels of C16:0 fatty acids in their seeds as the result of expression of these palmitoyl-ACP thioesterase sequences are also provided.

US Patent:

59687916, Oct 19, 1999

Filed:

Jun 1, 1995

Appl. No.:

8/458109

Inventors:

Huw Maelor Davies - Davis CA
Deborah Hawkins - Davis CA
Janet Nelson - Davis CA
Michael Lassner - Davis CA

Assignee:

Calgene, Inc. - Davis CA

International Classification:

C12P 764
C12N 910

US Classification:

435134

Abstract:

This invention relates to plant LPAATs, means to identify such proteins, amino acid and nucleic acid sequences associated with such protein, and methods to obtain, make and/or use such plant LPAATs. Purification, especially the removal of plant membranes and the substantial separation away from other plant proteins, and use of the plant LPAAT is provided, including the use of the protein as a tool in gene isolation for biotechnological applications. In addition, nucleic acid sequences encoding LPAAT protein regions are provided, and uses of such sequences for isolation of LPAAT genes from plants and for modification of plant triglyceride compositions are described.