Denise M Jackson

US Patent:

42372313, Dec 2, 1980

Filed:

Nov 13, 1979

Appl. No.:

6/093570

Inventors:

Denise M. Jackson - Chicago IL
Yoshihisa Tsuda - Highland Park IL

Assignee:

UOP Inc. - Des Plaines IL

International Classification:

C12N 992

US Classification:

435234

Abstract:

A process for purifying glucose isomerase comprises the steps of acid treatment and salt fractionation. An enzyme solution is treated with an acid, such as acetic acid, to a pH from about 3. 5 to about 5. The proteinaceous solids are collected and extracted with a buffer, such as imidazole, whose solution has a pH of about 6 to about 8. The solution is then collected and a salt, such as ammonium sulfate, is dissolved therein from about 40% to about 50% of its saturation point. The proteinaceous solids which form are removed and additional ammonium sulfate is dissolved to attain from about 41% to about 60% of its saturation point, followed by collection of the solids containing purified enzyme. A composition which preserves enzyme activity upon storage of glucose isomerase and which imparts resistance to thermal deactivation of said enzyme comprises an aqueous solution of glycerol, a buffer whose solution is at a pH of about 6 to about 8, divalent cobalt ions and magnesium ions.

US Patent:

45514321, Nov 5, 1985

Filed:

Nov 14, 1983

Appl. No.:

6/551646

Inventors:

Yoshihisa Tsuda - Wilmette IL
Denise M. Jackson - Chicago IL

Assignee:

UOP Inc. - Des Plaines IL

International Classification:

C12N 988
C12N 120
C12P 742
C12R 101

US Classification:

435232

Abstract:

A strain of Paracoccus denitrificans, NRRL B-15710, is an overproducer of a fumarase which is readily purified and relatively thermostable. The fumarase so produced converts fumaric acid to L-malic acid without formation of any significant amounts of a coproduct.

US Patent:

42568382, Mar 17, 1981

Filed:

Nov 13, 1979

Appl. No.:

6/093569

Inventors:

Denise M. Jackson - Chicago IL
Yoshihisa Tsuda - Highland Park IL
Vida Winans - Downers Grove IL

Assignee:

UOP Inc. - Des Plaines IL

International Classification:

C12N 992

US Classification:

435234

Abstract:

A process for purifying an enzyme such as glucose isomerase which comprises precipitating nucleic acids from a cell-free, heat-treated enzyme solution in a suitable buffer and chromatographing the supernatant on a cellulosic medium. Subsequent chromatography on a hydrophilic, molecular-sieve medium affords enzyme of about 90% purity.