Dennis J Gray

US Patent:

20050188432, Aug 25, 2005

Filed:

Feb 13, 2002

Appl. No.:

10/075105

Inventors:

Zhijian Li - Altamonte Springs FL, US
Dennis Gray - Howey in the Hills FL, US

International Classification:

A01H001/00
C12N015/82
C12N005/04

US Classification:

800278000, 435419000, 435468000

Abstract:

The present invention is directed to bidirectional promoter complexes that are effective for enhancing transcriptional activity of transgenes. The bidirectional promoters of the invention include a modified enhancer region with at least two core promoters on either side of the modified enhancer in a divergent orientation.

US Patent:

20070283455, Dec 6, 2007

Filed:

May 31, 2006

Appl. No.:

11/421122

Inventors:

Dennis Gray - Howey In The Hills FL, US
Manjul Dutt - Apopka FL, US

International Classification:

A01H 5/00
C12N 15/82

US Classification:

800278000, 435468000

Abstract:

Disclosed herein are methods of transforming grapevine. The methods involve the culturing of grapevine explant to induce shoot formation having meristematic regions and transforming meristematic tissue using Agrobacterium or particle bombardment.

US Patent:

20140082766, Mar 20, 2014

Filed:

Nov 19, 2013

Appl. No.:

14/084390

Inventors:

- GAINESVILLE FL, US
DENNIS J. GRAY - HOWEY-IN-THE-HILLS FL, US

International Classification:

C12N 15/82
C07K 14/435

US Classification:

800279, 530324, 800301, 435419

Abstract:

The subject invention pertains to methods and materials for enhancing microbial resistance in plants. Specifically exemplified herein are grapevines transformed with polynucleotides that express a peptide which confers antimicrobial activity.

US Patent:

20100138957, Jun 3, 2010

Filed:

Feb 1, 2008

Appl. No.:

12/524541

Inventors:

Zhijian T. Li - Altamonte Springs FL, US
Dennis J Gray - Howey-in-the-Hills FL, US

International Classification:

A01H 1/00
C07K 14/00
C07H 21/04
C12N 5/10
A01P 15/00
A01H 5/00
A61K 38/16
A61K 31/7088

US Classification:

800279, 530324, 536 231, 435419, 800298, 514 12, 514 44 R

Abstract:

The present invention concerns the development and utilization of hybrid lytic peptides derived from non-venomous molecular sources to confer a high level of sustainable resistance to phytopathogens in transgenic plants. In an exemplified embodiment, a composition of the invention comprises a cecropin-pleurocidin hybrid peptide of 27 amino acids. The peptide was designed based on optimization of critical molecular and physiochemical parameters. Peptides of the invention offer significantly enhanced antimicrobial activity and molecular properties associated with low cytotoxicity. Transgenic plants of grapevine () that express a peptide of the invention show antimicrobial activity against xylem-limited phytopathogenic bacterium at a level significantly higher than that from other existing lytic peptides. Thus, the hybrid peptides of the invention can be utilized as an antimicrobial agent for agricultural use.

US Patent:

20100024068, Jan 28, 2010

Filed:

Jun 13, 2006

Appl. No.:

11/922048

Inventors:

Zhijian T. Li - Altamonte Springs FL, US
Dennis J. Gray - Howey-in-the-Hills FL, US

International Classification:

A01H 1/00
C12N 5/10
C07K 14/00
C07H 21/04
A01H 5/00

US Classification:

800279, 435419, 530324, 536 231, 800301

Abstract:

The subject invention pertains to methods and materials for enhancing microbial resistance in plants. Specifically exemplified herein are grapevines transformed with polynucleotides that express a peptide which confers antimicrobial resistance.

US Patent:

20060035377, Feb 16, 2006

Filed:

Oct 4, 2005

Appl. No.:

11/243080

Inventors:

Jayasankar Subramanian - Tavares FL, US
Zhijian Li - Altamonde Springs FL, US
Dennis Gray - Howey-in-the-Hills FL, US

International Classification:

C12N 15/82
C12N 5/00
C12N 15/87
A01H 1/00

US Classification:

435430100, 800279000

Abstract:

The invention features a method of producing a grape somatic embryo having resistance to a plant pathogen, the method including the steps of (a) culturing a grape somatic embryo in a first liquid culture medium that includes a plant growth regulator and a phytotoxin from a plant pathogen; (b) exchanging the first liquid culture medium for a second liquid culture medium not including the phytotoxin; (c) recovering a living grape cell or grape cell cluster from the second liquid culture, the living cell or cell cluster being resistant to the pathogen; and (d) culturing the grape cell or grape cell cluster in a third culture medium to produce a grape somatic embryo.

US Patent:

20050246790, Nov 3, 2005

Filed:

Apr 15, 2005

Appl. No.:

11/107959

Inventors:

Dennis Gray - Howey-in-the Hills FL, US
Jayasankar Subramanian - Tavares FL, US
Richard Litz - Homestead FL, US

Assignee:

University of Florida - Gainsville FL

International Classification:

A01H001/00
C12N015/82
C12N005/04

US Classification:

800279000, 435419000

Abstract:

The invention features methods of producing a plant or a mature somatic embryo from an embryogenic cell or embryogenic culture. The method includes the steps of: (a) providing a liquid culture that includes an embryogenic cell; (b) recovering embryogenic cell from the culture; (c) transferring the embryogenic cell to a second culture; and (d) growing a mature somatic grape embryo from the embryogenic cell.

US Patent:

20050050592, Mar 3, 2005

Filed:

Sep 10, 2004

Appl. No.:

10/939061

Inventors:

Dennis Gray - Howey-in-the-Hills FL, US
Jayasankar Subramanian - Tavares FL, US
Richard Litz - Homestead FL, US

International Classification:

C12N005/04

US Classification:

800295000, 435419000

Abstract:

The invention features methods of producing a plant or a mature somatic embryo from an embryogenic cell or embryogenic culture. The method includes the steps of: (a) providing a liquid culture that includes an embryogenic cell; (b) recovering embryogenic cell from the culture; (c) transferring the embryogenic cell to a second culture; and (d) growing a mature somatic grape embryo from the embryogenic cell.