Diane T Russell

US Patent:

45827940, Apr 15, 1986

Filed:

Sep 15, 1982

Appl. No.:

6/418247

Inventors:

Diane H. Russell - Tucson AZ

Assignee:

The University of Arizona Foundation - Tucson AZ

International Classification:

C12Q 152

US Classification:

435 16

Abstract:

This application describes a method of determining the amount of organic polyamine in an aqueous solution to be assayed, which comprises (1) incubating a measured volume of said solution with a solution containing a protein with glutamine residues and a small, known amount of radiolabeled polyamine, in the presence of transglutaminase, (2) placing aliquots of the incubated solution on thin layer chromatographic strips treated with aqueous acid, (3) treating said strips with dilute aqueous acid solution to cause ascending chromatography, (4) cutting from the strips the sections where the aliquots had been applied thereto, (5) counting the radioactive scintillations of said sections, (6) carrying out steps (1) through (5) with the same volume of a standard solution containing a known amount of said polyamine, and (7) calculating the amount of polyamine in the solution to be assayed from the scintillation count made in the test procedure thereon in comparison to the scintillation count made in the test procedure conducted on the standard solution containing the known amount of said polyamine.

US Patent:

42017882, May 6, 1980

Filed:

Aug 11, 1978

Appl. No.:

5/932878

Inventors:

John J. Voorhees - Ann Arbor MI
Diane H. Russell - Tucson AZ

Assignee:

University Patents, Inc. - Stamford CT
The Regents of the University of Michigan - Ann Arbor MI

International Classification:

A61K 31275
A61K 3115

US Classification:

424304

Abstract:

A process and pharmaceutical compositions for alleviating proliferative skin diseases such as psoriasis, atopic dermatitis, etc. , comprising administering to humans or domesticated animals, topically and/or parenterally, and/or systemically a composition comprising a pharmaceutical carrier and at least one diamino compound selected from the diamines of saturated and unsaturated aliphatic hydrocarbons having 3 to 6 carbon atoms and the diamines of saturated monocarboxylic acids having 4 to 6 carbon atoms and the alkyl esters thereof containing from 1 to 4 carbon atoms. These diamino compounds are usable alone or in combination with certain prostaglandin compounds, certain phosphodiesterase inhibitor compounds and certain cyclic adenosine monophosphate compounds.