Dipak K Dube

US Patent:

20070111242, May 17, 2007

Filed:

Oct 18, 2006

Appl. No.:

11/582372

Inventors:

Dipak Dube - Seattle WA, US
Marshall Horwitz - Medina WA, US
Lawrence Loeb - Bellevue WA, US

International Classification:

C12Q 1/68
G06F 19/00

US Classification:

435006000, 702020000

Abstract:

A method of obtaining an oligonucleotide capable of carrying out a predetermined biological function. A heterogeneous pool of oligonucleotides, x+y+z nucleotides in length, is first generated. Each oligonucleotide has a 5′ randomized sequence, x nucleotides in length, a central preselected sequence, y nucleotides in length, and a 3′ randomized sequence, z nucleotides in length. The resulting heterogeneous pool contains nucleic acid sequences representing a random sampling of the 4possible sequences for oligonucleotides of the stated length. A random sampling of the heterogeneous pool of oligonucleotides is introduced Into a population of cells that do not exhibit the predetermined biological function. The population of engineered cells Is then screened for a subpopulation of cells exhibiting the predetermined biological function. From that subpopulation of cells Is Isolated an oligonucleotide containing the preselected sequence and capable of carrying out the predetermined biological function.

US Patent:

20060008806, Jan 12, 2006

Filed:

Jan 15, 2004

Appl. No.:

10/757590

Inventors:

Dipak Dube - Seattle WA, US
Marshall Horwitz - Meding WA, US
Lawrence Loeb - Bellevue WA, US

Assignee:

University of Washington - Seattle WA

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435006000, 435091200

Abstract:

A method of obtaining an oligonucleotide capable of carrying out a predetermined biological function. A heterogeneous pool of oligonucleotides, x+y+z nucleotides in length, is first generated. Each oligonucleotide has a 5′ randomized sequence, x nucleotides in length, a central preselected sequence, y nucleotides in length, and a 3′ randomized sequence, z nucleotides in length. The resulting heterogeneous pool contains nucleic acid sequences representing a random sampling of the 4possible sequences for oligonucleotides of the stated length. A random sampling of the heterogeneous pool of oligonucleotides is introduced into a population of cells that do not exhibit the predetermined biological function. The population of engineered cells is then screened for a subpopulation of cells exhibiting the predetermined biological function. From that subpopulation of cells is isolated an oligonucleotide containing the preselected sequence and capable of carrying out the predetermined biological function.