Don S Mcclure

US Patent:

8198084, Jun 12, 2012

Filed:

Jun 14, 2005

Appl. No.:

11/151647

Inventors:

Stephen F. Gorfien - Williamsville NY, US
Richard M. Fike - Clarence NY, US
Glenn P. Godwin - North Tonawanda NY, US
Joyce L. Dzimian - Depew NY, US
David A. Epstein - East Amherst NY, US
Dale Gruber - East Amherst NY, US
Don McClure - Indianapolis IN, US
Paul J. Price - Grand Island NY, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

C12N 5/00
C12N 5/02
C12N 5/07
C12N 5/10
C12N 11/00
C12N 11/16

US Classification:

435358, 435174, 435383, 435389, 435404

Abstract:

The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.

US Patent:

20070190046, Aug 16, 2007

Filed:

Feb 17, 2005

Appl. No.:

10/590411

Inventors:

Uma Kuchibhotia - Indianapolis IN, US
Don McClure - Indianapolis IN, US

International Classification:

A61K 39/395
C07K 16/18
C07H 21/04
C12P 21/06

US Classification:

424133100, 435069100, 435326000, 435320100, 530388100, 536023530

Abstract:

This present invention provides a composition that is suitable for administration to a human subject comprising an anti-Aβ antibody that is free of Aβ peptide or that has acceptably low levels thereof, free of non-human Aβ peptide or that has acceptably low levels thereof, or having an undetectable concentration of Aβ peptide.

US Patent:

20090280532, Nov 12, 2009

Filed:

May 8, 2009

Appl. No.:

12/463351

Inventors:

Stephen F. GORFIEN - Williamsville NY, US
Richard M. FIKE - Clarence NY, US
Glenn P. GODWIN - North Tonawanda NY, US
Joyce L. DZIMIAN - Lancaster NY, US
David A. EPSTEIN - East Amherst NY, US
Dale GRUBER - East Amherst NY, US
Don McCLURE - Indianapolis IN, US
Paul J. PRICE - Grand Island NY, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12P 21/00

US Classification:

435 691

Abstract:

The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.

US Patent:

20090280533, Nov 12, 2009

Filed:

May 8, 2009

Appl. No.:

12/463371

Inventors:

Stephen F. Gorfien - Williamsville NY, US
Richard M. Fike - Clarence NY, US
Glenn P. Godwin - North Tonawanda NY, US
Joyce L. Dzimian - Lancaster NY, US
David A. Epstein - East Amherst NY, US
Dale Gruber - East Amherst NY, US
Don McClure - Indianapolis IN, US
Paul J. Price - Grand Island NY, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12P 21/00

US Classification:

435 691

Abstract:

The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.

US Patent:

20130065275, Mar 14, 2013

Filed:

Sep 10, 2012

Appl. No.:

13/608803

Inventors:

Stephen F. GORFIEN - Williamsville NY, US
Richard M. Fike - Clarence NY, US
Glenn P. Godwin - Holbrook MA, US
Joyce L. Dzimian - Lancaster NY, US
David A. Epstein - East Amherst NY, US
Dale Gruber - Leesburg FL, US
Don McClure - Indianapolis IN, US
Paul J. Price - Mount Pleasant SC, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12N 5/075
C12N 7/00
C07K 2/00
C12P 21/00

US Classification:

435 691, 435358, 4352351, 530300

Abstract:

The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.

US Patent:

20150079659, Mar 19, 2015

Filed:

May 22, 2014

Appl. No.:

14/285047

Inventors:

- Carlsbad CA, US
Richard FIKE - Clarence NY, US
GLENN GODWIN - Holbrook MA, US
JOYCE WOLANSKE - Lancaster NY, US
David EPSTEIN - Philadelphia PA, US
Dale GRUBER - Leesburg FL, US
Don MCCLURE - Indianapolis IN, US
Paul PRICE - Mount Pleasant SC, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12N 5/071
C12N 9/24

US Classification:

435201, 435384, 435358

Abstract:

The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.