A process for cultivating photosynthetic microbes comprising Closed Systems for continuous cultivation and Open Systems for batch cultivation, in which (a) the Closed System Area occupies no more than 20% of the Total Land Area of the cultivation facility; (b) batch cultures in the Open Systems are initiated with an inoculum from the Closed Systems containing a cell biomass of no less than 5% of the carrying capacity of said Open System; (c) the doubling rate of said photosynthetic microbe is no less than once every 16 hours; and (d) the residence time of the batch culture in said Open System is no more than a period of 5 days.
Continuous-Batch Hybrid Process For Production Of Oil And Other Useful Products From Photosynthetic Microbes
Mark Edward Huntley - Kailua-Kona HI, US Donald C. Redalje - Diamondhead MS, US
International Classification:
C12P 5/00 C12N 1/20 C12M 1/00 C12N 1/12
US Classification:
435166, 435243, 4352521, 4352571, 4352921
Abstract:
A process for cultivating photosynthetic microbes comprising Closed Systems for continuous cultivation and Open Systems for batch cultivation, in which (a) the Closed System Area occupies no more than 20% of the Total Land Area of the cultivation facility; (b) batch cultures in the Open Systems are initiated with an inoculum from the Closed Systems containing a cell biomass of no less than 5% of the carrying capacity of said Open System; (c) the doubling rate of said photosynthetic microbe is no less than once every 16 hours; and (d) the residence time of the batch culture in said Open System is no more than a period of 5 days.
Continuous-Batch Hybrid Process For Production Of Oil And Other Useful Products From Photosynthetic Microbes
Mark Edward Huntley - Kailua-Kona HI, US Donald G. Redalje - Diamondhead MS, US
Assignee:
HR Biopetroleum, Inc. - Honolulu HI
International Classification:
C12M 1/42
US Classification:
4352921
Abstract:
A process for cultivating photosynthetic microbes comprising closed Systems for continuous cultivation and Open Systems for batch cultivation, in which (a) the Closed System Area occupies no more than 20% of the Total Land Area of the cultivation facility; (b) batch cultures in the Open Systems are initiated with an inoculum from the Closed Systems containing a cell biomass of no less than 5% of the carrying capacity of said Open System; (c) the doubling rate of said photosynthetic microbe is no less than once every 16 hours; and (d) the residence time of the batch culture in said Open System is no more than a period of 5 days.
Mark E. Huntley - Honolulu HI Pearn P. Niiler - La Jolla CA Donald Redalje - Pass Christian MS
Assignee:
Aquasearch, Inc. - La Jolla CA
International Classification:
C12Q 300 C12M 136
US Classification:
435 3
Abstract:
A control method for operating aqueous microorganism growth processes is disclosed which can maintain viable growth conditions for many types of microorganisms which have heretofore not been easily reproduced in commercially valuable quantities. The primary control parameters are the degree of turbulence in the aqueous growth medium and the scale of the apparatus relative to the scale of the turbulent eddies in vessels which are partially filled with the aqueous medium directly affect conditions which are required for optimum growth: light exposure, nutrient supply, sedimentation rate, bulk temperature, gas exchange rate and cell integrity. These control elements can be cast in terms of the Reynolds number (N. sub. Re) and controlled dimensions of the apparatus (L. sub. K) in relationship to the scales of turbulent eddies to define the dissipation. lambda. sub. K as L. sub. K /L.