Edward J Doherty

US Patent:

57767475, Jul 7, 1998

Filed:

May 23, 1995

Appl. No.:

8/447778

Inventors:

Malcolm Schinstine - Bensalem PA
Molly S. Shoichet - Toronto, CA
Frank T. Gentile - Warwick RI
Joseph P. Hammang - Barrington RI
Laura M. Holland - Horsham PA
Brian M. Cain - Everett MA
Edward J. Doherty - Mansfield MA
Shelley R. Winn - Smithfield RI
Patrick Aebischer - Lutry, CH

Assignee:

Cytotherapeutics, Inc.

International Classification:

C12N 1102
C12N 1108
C12N 1106
C12N 1104

US Classification:

435177

Abstract:

This invention relates to methods and compositions of controlling cell distribution within a bioartificial organ by exposing the cells to a treatment that inhibits cell proliferation, promotes cell differentiation, or affects cell attachment to a growth surface within the bioartificial organ. Such treatments include (1) genetically manipulating cells, (2) exposing the cells to a proliferation-inhibiting compound or a differentiation-inducing compound or removing the cells from exposure to a proliferation-stimulating compound or a differentiation-inhibiting compound; exposing the cells to irradiation, and (3) modifying a growth surface of the BAO with ECM molecules, molecules affecting cell proliferation or adhesion, or an inert scaffold, or a combination thereof. These treatments may be used in combination. A particular embodiment is directed to derivatizing or adsorbing polyethylene oxide-poly(dimethylsiloxane) copolymer (PEO-PDMS) onto a surface within the bioartificial organ to inhibit cellular attachment.