Elizabeth F Collins

US Patent:

20140024024, Jan 23, 2014

Filed:

Jul 17, 2012

Appl. No.:

13/551190

Inventors:

Anup Sood - Clifton Park NY, US
Michael John Gerdes - Albany NY, US
Antti Eljas Seppo - Altamont NY, US
Wei Gao - Clifton Park NY, US
Fiona Mary Ginty - Saratoga Springs NY, US
Elizabeth Mary Collins - Albany NY, US

Assignee:

General Electric Company - Schenectady NY

International Classification:

C12Q 1/68

US Classification:

435 611

Abstract:

Novel methods of probing multiple targets in a biological sample are provide whereby the targets are DNA, RNA and protein. The method comprises subjecting the sample to an in situ hybridization reaction using a labeled nucleic acid probe that binds an RNA target, observing a signal, and optionally removing the signal. The method further comprises an antigen retrieval protocol, observing a signal, removing the signal, and optionally applying a protease treatment to access the sample's DNA targets by subjecting the sample to an in situ hybridization reaction using a labeled nucleic acid probe, observing a signal from the labeled DNA targets, and optionally removing the signal.

Address:

4000 NW 51 St APT L212, Gainesville, FL 32606

VIN:

JHMCP26348C008620

Make:

HONDA

Model:

ACCORD

Year:

2008