Elizabeth R Gray

US Patent:

20210340519, Nov 4, 2021

Filed:

Jun 2, 2021

Appl. No.:

17/336889

Inventors:

- Seattle WA, US
- Salt Lake City UT, US
Joerg VOTTELER - Salt Lake City UT, US
Yang HSIA - Seattle WA, US
David BAKER - Seattle WA, US
Jacob BALE - Seattle WA, US
Marc LAJOIE - Seattle WA, US
Gabriel BUTTERFIELD - Seattle WA, US
Elizabeth GRAY - Seattle WA, US
Daniel STETSON - Seattle WA, US

International Classification:

C12N 9/88
C07K 14/00
C07K 14/435

Abstract:

The application discloses multimeric assemblies including multiple oligomeric substructures, where each oligomeric substructure includes multiple proteins that self-interact around at least one axis of rotational symmetry, where each protein includes one or more polypeptide-polypeptide interface (“O interface”); and one or more polypeptide domain that is capable of effecting membrane scission and release of an enveloped multimeric assembly from a cell by recruiting the ESCRT machinery to the site of budding by binding to one or more proteins in the eukaryotic ESCRT complex (“L domain”); and where the multimeric assembly includes one or more subunits comprising one or more polypeptide domain that is capable of interacting with a lipid bilayer (“M domain”), as well as membrane-enveloped versions of the multimeric assemblies.

US Patent:

20200224186, Jul 16, 2020

Filed:

Nov 6, 2019

Appl. No.:

16/676253

Inventors:

- Seattle WA, US
- Salt Lake City UT, US
Joerg VOTTELER - Salt Lake City UT, US
Yang HSIA - Seattle WA, US
David BAKER - Seattle WA, US
Jacob BALE - Seattle WA, US
Marc LAJOIE - Seattle WA, US
Gabriel BUTTERFIELD - Seattle WA, US
Elizabeth GRAY - Seattle WA, US
Daniel STETSON - Seattle WA, US

International Classification:

C12N 9/88
C07K 14/00
C07K 14/435

Abstract:

The application discloses multimeric assemblies including multiple oligomeric substructures, where each oligomeric substructure includes multiple proteins that self-interact around at least one axis of rotational symmetry, where each protein includes one or more polypeptide-polypeptide interface (“O interface”); and one or more polypeptide domain that is capable of effecting membrane scission and release of an enveloped multimeric assembly from a cell by recruiting the ESCRT machinery to the site of budding by binding to one or more proteins in the eukaryotic ESCRT complex (“L domain”); and where the multimeric assembly includes one or more subunits comprising one or more polypeptide domain that is capable of interacting with a lipid bilayer (“M domain”), as well as membrane-enveloped versions of the multimeric assemblies.

US Patent:

20180030429, Feb 1, 2018

Filed:

Feb 29, 2016

Appl. No.:

15/541201

Inventors:

- Seattle WA, US
- Salt Lake City UT, US
Joerg VOTTELER - Salt Lake City UT, US
Yang HSIA - Seattle WA, US
David BAKER - Seattle WA, US
Jacob BALE - Seattle WA, US
Marc LAJOIE - Seattle WA, US
Gabriel BUTTERFIELD - Seattle WA, US
Elizabeth GRAY - Seattle WA, US
Daniel STETSON - Seattle WA, US

International Classification:

C12N 9/88

Abstract:

The application discloses multimeric assemblies including multiple oligomeric substructures, where each oligomeric substructure includes multiple proteins that self-interact around at least one axis of rotational symmetry, where each protein includes one or more polypeptide-polypeptide interface (“O interface”); and one or more polypeptide domain that is capable of effecting membrane scission and release of an enveloped multimeric assembly from a cell by recruiting the ESCRT machinery to the site of budding by binding to one or more proteins in the eukaryotic ESCRT complex (“L domain”); and where the multimeric assembly includes one or more subunits comprising one or more polypeptide domain that is capable of interacting with a lipid bilayer (“M domain”), as well as membrane-enveloped versions of the multimeric assemblies.