Elizabeth R Ward

US Patent:

20200362000, Nov 19, 2020

Filed:

Mar 13, 2020

Appl. No.:

16/818827

Inventors:

- Cambridge MA, US
Cristina Zamora - Medford MA, US
Elizabeth Ward - Somerville MA, US

Assignee:

Massachusetts Institute of Technology - Cambridge MA

International Classification:

C07K 14/47

Abstract:

Glycan-binding proteins, and compositions thereof, are generally described, including methods of making and using such proteins. The proteins may include scaffolds based on easily evolvable DNA-binding proteins, with binding sites able to specifically bind to mono- or disaccharides, such as monosaccharide-binding determinants, disaccharide-binding determinants, more complex carbohydrates, etc. In certain aspects, a protein may be generated starting from a small DNA-binding protein, such as Sso7d. Such glycan-binding proteins may have numerous applications, including in enzyme-linked immunosorbent assays (ELISAs), glycan characterization, cell selection, flow cytometry, histology, imaging, arrays, affinity purification, enzyme-linked visualization, binding to a target for pharmaceutical purposes, etc.