Elizabeth R Waters

US Patent:

20060259993, Nov 16, 2006

Filed:

Jan 28, 2003

Appl. No.:

10/502832

Inventors:

Nelson Barton - SAN DIEGO CA, US
David Weiner - Del Mar CA, US
William Greenberg - San Diego CA, US
Samantha Luu - San Diego CA, US
Kristine Chang - San Diego CA, US
Elizabeth Waters - San Diego CA, US

International Classification:

A01K 67/027
A01H 5/00
C12N 1/21
C12Q 1/68
C07H 21/04
C07D 501/00
C12N 9/80
C07D 501/14
C12N 15/74
C12N 15/82
C12P 35/02

US Classification:

800018000, 536023200, 435006000, 435228000, 435252300, 435471000, 800288000, 540222000, 435051000

Abstract:

This invention provides amidases, polynucleotides encoding the amidases, methods of making and using these polynucleotides and polypeptides. In one aspect, the invention provides enzymes having secondary amidase activity, e.g., having activity in the hydrolysis of amides, including enzymes having peptidase, protease and/or hydantoinase activity. In alternative aspects, the enzymes of the invention can be used to used to increase flavor in food (e.g., enzyme ripened cheese), promote bacterial and fungal killing, modify and de-protect fine chemical intermediates, synthesize peptide bonds, carry out chiral resolutions, hydrolyze Cephalosporin C. The enzymes of the invention can be used to generate 7-aminocephalosporanic acid (7-ACA) and semi-synthetic cephalosporin antibiotics, including caphalothin, cephaloridine and cefuroxime. The enzymes of the invention can be used as antimicrobial agents, e.g., as cell wall hydrolytic agents. The invention also provides a fluorescent amidase substrate comprising 7-(ε-D-2-aminoadipoyladipoylamido)-4-methylcoumarin.

US Patent:

20070056053, Mar 8, 2007

Filed:

Mar 18, 2004

Appl. No.:

10/550155

Inventors:

Kevin Gray - San Diego CA, US
James Garrett - San Diego CA, US
Nahla Aboushadi - Katy TX, US
Ryan Knowles - Chula Vista CA, US
Eileen O'Donoghue - San Diego CA, US
Elizabeth Waters - San Diego CA, US

Assignee:

DIVERSA CORPORATION - San Diego CA

International Classification:

A01K 67/027
C12Q 1/68
C07H 21/04
C12P 21/06
C12N 9/42
A01H 1/00
C12N 5/04

US Classification:

800018000, 435069100, 435209000, 435320100, 435325000, 536023200, 435006000, 800284000, 435419000

Abstract:

The invention is directed to polypeptides having a glucosidase activity, including an alpha-glucosidase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention are used as alpha-glucosidases to catalyze the hydrolysis of starch into sugars, e.g., to convert liquefied starch to glucose. In one aspect, the polypeptides of the invention can catalyze the hydrolysis of both alpha-(1,4) and alpha-(1,6) glucose linkages. In one aspect, the polypeptides of the invention can catalyze the hydrolysis of both malto-oligosaccharides and liquefied starch.

US Patent:

20080168572, Jul 10, 2008

Filed:

May 1, 2003

Appl. No.:

10/428340

Inventors:

Karl O. Stetter - Regensubrg, DE
Elizabeth Waters - San Diego CA, US
Keith Kretz - San Marcos CA, US
Mircea Podar - San Diego CA, US
Toby Richardson - San Diego CA, US
Michiel Noordewier - San Diego CA, US

Assignee:

Diversa Corporation - San Diego CA

International Classification:

A01K 67/00
C07H 21/04
C07K 16/00
C12N 15/00
C12N 1/34
C12Q 1/68
C40B 50/06
G01N 33/48
G06G 7/48
C40B 40/10
G01N 33/53
C12N 5/06
C12N 5/00
A01H 5/00

US Classification:

800 8, 536 232, 536 243, 536 2433, 530350, 4353201, 435325, 800298, 536 245, 435375, 435183, 5303873, 506 18, 435 71, 435 6, 703 11, 702 19, 536 2541, 506 26, 800288, 4352521

Abstract:

The invention provides a genome of the hyperthermophile , polypeptides, including enzymes, structural protein and binding proteins, derived from this genome, polynucleotides encoding these polypeptides, methods of making and using these polynucleotides and polypeptides. The invention also provides isolated hyperthermophile

US Patent:

20040023257, Feb 5, 2004

Filed:

Jan 28, 2003

Appl. No.:

10/354437

Inventors:

Nelson Barton - San Diego CA, US
David Weiner - Del Mar CA, US
William Greenberg - San Diego CA, US
Samantha Luu - San Diego CA, US
Kristine Chang - San Diego CA, US
Elizabeth Waters - San Diego CA, US

Assignee:

Diversa Corporation - San Diego CA

International Classification:

C12Q001/68
G06F019/00
G01N033/48
G01N033/50
C07H021/04
C12N009/80

US Classification:

435/006000, 435/069100, 435/228000, 435/320100, 435/325000, 536/023200, 702/019000

Abstract:

This invention provides amidases, polynucleotides encoding the amidases, methods of making and using these polynucleotides and polypeptides. In one aspect, the invention provides enzymes having secondary amidase activity, e.g., having activity in the hydrolysis of amides, including enzymes having peptidase, protease and/or hydantoinase activity. In alternative aspects, the enzymes of the invention can be used to used to increase flavor in food (e.g., enzyme ripened cheese), promote bacterial and fungal killing, modify and de-protect fine chemical intermediates, synthesize peptide bonds, carry out chiral resolutions, hydrolyze Cephalosporin C. The enzymes of the invention can be used to generate 7-aminocephalosporanic acid (7-ACA) and semi-synthetic cephalosporin antibiotics, including caphalothin, cephaloridine and cefuroxime. The enzymes of the invention can be used as antimicrobial agents, e.g., as cell wall hydrolytic agents. The invention also provides a fluorescent amidase substrate comprising 7-( -D-2-aminoadipoylamido)-4-methylcoumarin.