Elizabeth R Wheeler

US Patent:

20140057276, Feb 27, 2014

Filed:

Nov 1, 2013

Appl. No.:

14/070395

Inventors:

Roald N. Leif - San Ramon CA, US
Elizabeth Wheeler - Livermore CA, US

Assignee:

Lawrence Livermore National Security, LLC - Livermore CA

International Classification:

C12Q 1/68

US Classification:

435 612

Abstract:

A simulant that includes a carrier and DNA encapsulated in the carrier. Also a method of making a simulant including the steps of providing a carrier and encapsulating DNA in the carrier to produce the simulant.

US Patent:

20180246076, Aug 30, 2018

Filed:

Feb 21, 2018

Appl. No.:

15/901766

Inventors:

- Livermore CA, US
Chao Huang - San Jose CA, US
Anna Nikolaevna Ivanovskaya - Mountain View CA, US
Kris Kulp - Livermore CA, US
Elizabeth K. Wheeler - Livermore CA, US

International Classification:

G01N 33/483
C12M 1/34

Abstract:

A sensor array for simultaneously measuring electrophysiological responses and contractility responses of a tissue includes: a substrate; a multi-electrode array (MEA) disposed in or on the substrate; and a plurality of interdigitized electrodes (IDEs) disposed in or on the substrate. The MEA and the IDEs are interpenetrating within a plane substantially parallel to an upper surface of the substrate. Systems for measuring such responses using the sensor array may also include contacts and/or connectors for interfacing with external control devices, electrodes, and cell culture components such as a chamber and lid. Fabrication techniques for making such systems include: forming a sensor array comprising an interpenetrating arrangement of IDEs and electrodes of a MEA in or on a substrate surface; forming a plurality of contacts for interfacing the system with one or more external devices in or on the substrate surface; and forming leads between the plurality of contacts and the sensor array.

US Patent:

20150361490, Dec 17, 2015

Filed:

Apr 2, 2015

Appl. No.:

14/677840

Inventors:

- Livermore CA, US
Roald N. Leif - San Ramon CA, US
Elizabeth Wheeler - Livermore CA, US

International Classification:

C12Q 1/68

Abstract:

In one embodiment, a product includes a plurality of particles, each particle including: a carrier that includes a non-toxic material; and at least one DNA barcode coupled to the carrier, where the particles each have a diameter in a range from about 1 nanometer to about 100 microns.

US Patent:

20140322701, Oct 30, 2014

Filed:

Apr 29, 2014

Appl. No.:

14/265019

Inventors:

- Livermore CA, US
Haiyin Chen - San Ramon CA, US
Bryan D. Hudson - Livermore CA, US
Kris Kulp - Livermore CA, US
Margaret W. Mcnerney - Pleasanton CA, US
Satinderpall S. Pannu - Pleasanton CA, US
Fang Qian - Santa Cruz CA, US
Angela C. Tooker - Dublin CA, US
Elizabeth K. Wheeler - Livermore CA, US

Assignee:

Lawrence Livermore National Security, LLC - Livermore CA

International Classification:

C12M 3/06
C12M 3/00

US Classification:

435 5, 4352891, 4352872, 4352879, 4353031, 435325, 435 29, 435 61

Abstract:

In one embodiment, a system includes a bioreactor coupled to a substrate. The bioreactor includes: a plurality of walls defining a reservoir; a plurality of fluidic channels in at least some of the walls; a fluidic inlet in fluidic communication with the reservoir via, the fluidic channels; a fluidic outlet in fluidic communication with the reservoir via the fluidic channels; and one or more sensors coupled to the reservoir, each sensor being configured to detect one or more of: environmental conditions in the reservoir; and physiological conditions of one or more cells optionally present in the reservoir. In another embodiment, a method includes delivering media to a reservoir of a bioreactor; delivering a, plurality of cells to the reservoir, controlling a reservoir temperature and a reservoir gas composition; using one or more of the sensors to monitor environmental and physiological conditions; and reporting the environmental and/or physiological conditions.