Elizabeth M Wilkins

US Patent:

6448091, Sep 10, 2002

Filed:

Nov 7, 1994

Appl. No.:

08/335183

Inventors:

Richard J. Massey - Rockville MD
Gary F. Blackburn - Gaithersburg MD
Elizabeth W. Wilkins - Germantown MD
Haresh P. Shah - Gaithersburg MD

Assignee:

IGEN International, Inc. - Gaithersburg MD

International Classification:

G01N 2176

US Classification:

436501, 436524, 436526, 436528, 436172

Abstract:

Methods and apparatus for performing a binding assay for an analyte of interest present in a sample are described. The methods include the steps of: forming a composition containing the sample, an assay-performance-substance which contains a component linked to a label compound capable of chemiluminescing when triggered, and a plurality of particles capable of specifically binding with the analyte and/or the assay-performance-substance; incubating the composition to form a complex which includes a particle and the labeled component; collecting the complex in a collection zone; introducing into the collection zone a trigger capable of triggering the label such that the label luminesces; and measuring the emitted luminescence to measure the presence of the analyte of interest in the sample.

US Patent:

20030008339, Jan 9, 2003

Filed:

Sep 5, 2002

Appl. No.:

10/235127

Inventors:

Richard J. Massey - Rockville MD, US
Gary Blackburn - Gaithersburg MD, US
Elizabeth W. Wilkins - Germantown MD, US
Haresh P. Shah - Gaithersburg MD, US

Assignee:

Igen, Inc.

International Classification:

C12Q001/54

US Classification:

435/014000

Abstract:

What is described are methods and apparatus for performing a binding assay for an analyte of interest present in a sample. The methods include the steps of: forming a composition containing the sample, an assay-performance-substance which contains a component linked to a label compound capable of chemiluminescing when triggered, and a plurality of particles capable of specifically binding with the analyte and/or the assay-performance-substance; incubating the composition to form a complex which includes a particle and the labeled component; collecting the complex in a collection zone; introducing into the collection zone a trigger capable of triggering the label such that the label luminesces; and measuring the emitted luminescence to measure the presence of the analyte of interest in the sample.

US Patent:

57704597, Jun 23, 1998

Filed:

Dec 1, 1994

Appl. No.:

8/348749

Inventors:

Richard J. Massey - Rockville MD
Gary F. Blackburn - Gaithersburg MD
Elizabeth W. Wilkins - Germantown MD
Jonathan K. Leland - Laurel MD

Assignee:

IGEN International, Inc. - Gaithersburg MD

International Classification:

G01N 3353

US Classification:

436526

Abstract:

What is described are methods and apparatus for performing a binding assay for an analyte of interest present in a sample. The methods include the steps of: forming a composition containing said sample, an assay-performance-substance which contains a component linked to a label compound capable of chemiluminescing when triggered, and a plurality of coated magnetic particles capable of specifically binding with the analyte and/or said assay-performance-substance; incubating said composition to form a complex which includes a particle and said labeled component; magnetically collecting said complex at the surface of an electrode; inducing said label to luminesce by contacting it with a trigger, said trigger being formed in-situ by conversion of a precursor molecule upon introduction of electrochemical energy; and measuring the emitted luminescence to measure the presence of the analyte of interest in the sample.

US Patent:

57469741, May 5, 1998

Filed:

Jun 6, 1995

Appl. No.:

8/467028

Inventors:

Richard J. Massey - Rockville MD
Gary F. Blackburn - Gaithersburg MD
Elizabeth W. Wilkins - Germantown MD
Jonathan K. Leland - Laurel MD

Assignee:

IGEN International, Inc. - Gaithersburg MD

International Classification:

G01N 2166

US Classification:

422 52

Abstract:

What is described are methods and apparatus for performing a binding assay for an analyte of interest present in a sample. The methods include the steps of: forming a composition containing said sample, an assay-performance-substance which contains a component linked to a label compound capable of chemiluminescing when triggered, and a plurality of coated magnetic particles capable of specifically binding with the analyte and/or said assay-performance-substance; incubating said composition to form a complex which includes a particle and said labeled component; magnetically collecting said complex at the surface of an electrode; inducing said label to luminesce by contacting it with a trigger, said trigger being formed in-situ by conversion of a precursor molecule upon introduction of electrochemical energy; and measuring the emitted luminescence to measure the presence of the analyte of interest in the sample.

US Patent:

57980837, Aug 25, 1998

Filed:

Jun 6, 1995

Appl. No.:

8/469464

Inventors:

Richard J. Massey - Rockville MD
Gary F. Blackburn - Gaithersburg MD
Elizabeth W. Wilkins - Germantown MD
Haresh P. Shah - Gaithersburg MD

Assignee:

IGEN International, Inc. - Gaithersburg MA

International Classification:

G01N 2176

US Classification:

422 52

Abstract:

What is described are methods and apparatus for performing a binding assay for an analyte of interest present in a sample. The methods include the steps of: forming a composition containing the sample, an assay-performance-substance which contains a component linked to a label compound capable of chemiluminescing when triggered, and a plurality of particles capable of specifically binding with the analyte and/or the assay-performance-substance; incubating the composition to form a complex which includes a particle and the labeled component; collecting the complex in a collection zone; introducing into the collection zone a trigger capable of triggering the label such that the label luminesces; and measuring the emitted luminescence to measure the presence of the analyte of interest in the sample.