Ellen F Guthrie

US Patent:

7319014, Jan 15, 2008

Filed:

Feb 20, 2004

Appl. No.:

10/545905

Inventors:

Ellen Guthrie - Andover MA, US
Theodore Davis - Boxford MA, US
Jack Benner, II - South Hamilton MA, US

Assignee:

New England Biolabs, Inc. - Ipswich MA

International Classification:

C12Q 1/42
C12P 21/06
C12N 9/16

US Classification:

435 21, 435 681, 435196

Abstract:

Compositions for an alkaline phosphatase and methods for over-expression and purification of thermolabile Antarctic phosphatase (TAP) are provided. Uses for TAP include dephosphorylation of nucleic acids, sugars, peptides and proteins. TAP as described herein has advantages over phosphatases from other sources with respect to thermolability at 65 C. and efficiency of dephosphorylation activity at approximately neutral pH.

US Patent:

20110053215, Mar 3, 2011

Filed:

Apr 7, 2009

Appl. No.:

12/988344

Inventors:

Dimitris Koutsioulis - Larissa, GR
Ellen Guthrie - Andover MA, US

Assignee:

NEW ENGLAND BIOLABS, INC. - Ipswich MA

International Classification:

C12P 21/00
C12N 9/24
C07H 21/00

US Classification:

435 681, 435200, 536 232

Abstract:

Methods and compositions have been described that relate to a newly identified polypeptide family wherein each member has O-glycosidase activity and specified sequence characteristics. This family of enzymes can be used for example for cleaving O-linked glycans and for synthesis of neoglycopeptides or neoglycoproteins.

US Patent:

52926516, Mar 8, 1994

Filed:

May 12, 1992

Appl. No.:

7/880913

Inventors:

Ellen P. Guthrie - Andover MA
Elizabeth M. Van Cott - Malden MA
Christopher H. Taron - Marblehead MA

Assignee:

New England Biolabs, Inc. - Beverly MA

International Classification:

C12N 922
C12N 1555

US Classification:

435199

Abstract:

The present invention is directed to a method for cloning and producing the NaeI restriction endonuclease by 1) introducing the restriction endonuclease gene from Nocardia aerocolonigenes into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the NaeI restriction endonuclease activity, and 3) purifying the NaeI restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the NaeI restriction endonuclease activity.

US Patent:

52623181, Nov 16, 1993

Filed:

Aug 20, 1992

Appl. No.:

7/932454

Inventors:

Ellen P. Guthrie - Andover MA

Assignee:

New England Biolabs, Inc. - Beverly MA

International Classification:

C12N 922
C12N 1555

US Classification:

435199

Abstract:

The present invention is directed to a method for cloning and producing the SphI restriction endonuclease by 1) introducing the restriction endonuclease gene from Streptomyces phaeochromogenes into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the SphI restriction endonuclease activity, and 3) purifying the SphI restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the SphI restriction endonuclease activity.

US Patent:

52886964, Feb 22, 1994

Filed:

Sep 7, 1990

Appl. No.:

7/578822

Inventors:

Ellen P. Guthrie - Swampscott MA
Marta M. Meda - Beverly MA

Assignee:

New England Biolabs, Inc. - Beverly MA

International Classification:

C12N 910
C12N 1554
C12N 1570
C12N 1576

US Classification:

435199

Abstract:

The present invention is directed to a method for cloning and producing the SacII restriction endonuclease by 1) introducing the restriction endonuclease gene from Streptomyces achromogenes into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the SacII restriction endonuclease activity, and 3) purifying the SacII restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the SacII restriction endonuclease activity.

US Patent:

20170138959, May 18, 2017

Filed:

Dec 28, 2016

Appl. No.:

15/392908

Inventors:

- Ipswich MA, US
Ellen Guthrie - Andover MA, US
Christopher H. Taron - Essex MA, US
John Buswell - Byfield MA, US

Assignee:

New England Biolabs, Inc. - Ipswich MA

International Classification:

G01N 33/68
C07K 1/12
C07K 16/28
C07K 16/24
C12N 9/96
C12N 9/80
C12N 9/76
C12N 9/10
G01N 33/535
G01N 33/543
C07K 16/00
C07K 14/525

Abstract:

Compositions and methods are provided for efficiently preparing a completely deglycosylated antibody where efficiency is measured in relative amounts of reagents in soluble or lyophilized form, and time and temperature of the reaction. Compositions and methods are also provided for separating substantially all N-linked glycans from a glycosylated antibody and for preserving functionality of the antibody. The methods are compatible with glycan labeling and protease digestion without the need for prior purification steps.

US Patent:

20150346194, Dec 3, 2015

Filed:

May 29, 2015

Appl. No.:

14/725614

Inventors:

- Ipswich MA, US
Ellen Guthrie - Andover MA, US
Christopher H. Taron - Essex MA, US
John Buswell - Byfield MA, US

Assignee:

NEW ENGLAND BIOLABS, INC. - Ipswich MA

International Classification:

G01N 33/531
C12N 9/96
C07K 14/525
C07K 16/00
C07K 16/28
G01N 33/53
C12P 21/00

Abstract:

Compositions and methods are provided for efficiently preparing a completely deglycosylated antibody where efficiency is measured in relative amounts of reagents in soluble or lyophilized form, and time and temperature of the reaction. Compositions and methods are also provided for separating substantially all N-linked glycans from a glycosylated antibody and for preserving functionality of the antibody. The methods are compatible with glycan labeling and protease digestion without the need for prior purification steps.