Elliot E Hui

US Patent:

20100167330, Jul 1, 2010

Filed:

Mar 10, 2008

Appl. No.:

12/449994

Inventors:

Sangeeta N. Bhatia - Lexington MA, US
Elliot Hui - Irvine CA, US

International Classification:

C12Q 1/02
C12N 5/071
C12N 5/0735
C12N 5/073
C12N 5/02
C12N 5/074

US Classification:

435 29, 435366, 435368, 435369, 435370, 435371, 435372, 435373

Abstract:

The development and function of living tissues depends largely on interactions between cells that can vary in both time and space; however, temporal control of cell-cell interaction is experimentally challenging. By employing a micromachined silicon substrate with moving parts, herein is disclosed the dynamic regulation of cell-cell interactions via direct manipulation of adherent cells with micron-scale precision. The inventive devices and methods allow mechanical control of both tissue composition and spatial organization. The inventive device and methods enable the investigation of dynamic cell-cell interaction in a multitude of applications, such as intercellular communication, spanning embryogenesis, homeostasis, and pathogenic processes.

US Patent:

20200283723, Sep 10, 2020

Filed:

May 6, 2020

Appl. No.:

16/868474

Inventors:

- Oakland CA, US
Janice De Jesus - San Diego CA, US
Elliot En-Yu Hui - Irvine CA, US
Jiang Li - Diamond Bar CA, US

Assignee:

THE REGENTS OF THE UNIVERSITY OF CALIFORNIA - Oakland CA

International Classification:

C12M 1/00
G01N 1/28
C12M 1/40
C12M 3/00
B01L 3/00

Abstract:

A tissue dissociation device includes an inlet coupled to a first stage having a single channel having an upstream end and a downstream end; a plurality of serially arranged intermediate stages, wherein a first intermediate stage of the plurality is fluidically coupled to the downstream end of the first stage, and wherein each subsequent intermediate stage of the plurality has an increasing number of channels (with channels of smaller dimensions); and an outlet coupled to a last stage of the intermediate stages.

US Patent:

20190070605, Mar 7, 2019

Filed:

Aug 28, 2018

Appl. No.:

16/115434

Inventors:

- Oakland CA, US
Xiaolong Qiu - Chula Vista CA, US
Elliot Hui - South Pasadena CA, US
Amrith Karunaratne - Irvine CA, US
Erik Werner - Irvine CA, US

Assignee:

THE REGENTS OF THE UNIVERSITY OF CALIFORNIA - Oakland CA

International Classification:

B01L 3/00
C12M 1/33
G01N 1/28

Abstract:

A microfluidic device uses hydrodynamic shear forces on a sample to improve the speed and efficiency of tissue digestion is disclosed. The microfluidic channels are designed to apply hydrodynamic shear forces at discrete locations on tissue specimens up to 1 cm in length and 1 mm in diameter, thereby accelerating digestion through hydrodynamic shear forces and improved enzyme-tissue contact. Experiments using animal organs show that the digestion device with hydro-mincing capabilities is superior to conventional scalpel mincing and digestion based on recovery of DNA and viable single cells. The microfluidic digestion device can eliminate or reduce the need to mince tissue samples with a scalpel, while reducing sample processing time and preserving cell viability. Another advantage is that downstream microfluidic operations could be integrated to enable advanced cell processing and analysis capabilities. The device may be used in research and clinical settings to promote single cell-based analysis technologies, as well as to isolate primary, progenitor, and stem cells for use in the fields of tissue engineering and regenerative medicine.

US Patent:

20180023552, Jan 25, 2018

Filed:

Sep 21, 2017

Appl. No.:

15/711946

Inventors:

Elliot En-Yu Hui - South Pasadena CA, US
Philip N. Duncan - Fullerton CA, US
Transon V. Nguyen - Cambridge MA, US

International Classification:

F04B 19/00
F04B 53/10
F16K 99/00
F04B 9/12

Abstract:

Microfluidic oscillator circuits and pumps for microfluidic devices are provided. The microfluidic pump may include a plurality of fluid valves and a microfluidic oscillator circuit having an oscillation frequency. The fluid valves may be configured to move fluids. Each fluid valve may be connected to a node of the microfluidic oscillator circuit. The pumps may be driven by the oscillator circuits such that fluid movement is accomplished entirely by circuits on a microfluidic chip, without the need for off-chip controls.

US Patent:

20170131187, May 11, 2017

Filed:

Jan 23, 2017

Appl. No.:

15/413201

Inventors:

- Oakland CA, US
Janice De Jesus - San Diego CA, US
Elliot En-Yu Hui - Irvine CA, US
Jiang Li - Diamond Bar CA, US

Assignee:

THE REGENTS OF THE UNIVERSITY OF CALIFORNIA - Oakland CA

International Classification:

G01N 1/28

Abstract:

A tissue dissociation device includes an inlet coupled to a first stage having a single channel having an upstream end and a downstream end; a plurality of serially arranged intermediate stages, wherein a first intermediate stage of the plurality is fluidically coupled to the downstream end of the first stage, and wherein each subsequent intermediate stage of the plurality has an increasing number of channels (with channels of smaller dimensions); and an outlet coupled to a last stage of the intermediate stages.

US Patent:

20140377866, Dec 25, 2014

Filed:

Jun 19, 2014

Appl. No.:

14/309720

Inventors:

- Oakland CA, US
Janice De Jesus - San Diego CA, US
Elliot En-Yu Hui - Irvine CA, US
Jiang Li - Diamond Bar CA, US

International Classification:

C12N 5/09
C12M 1/40
C12M 1/00
G01N 1/28

US Classification:

435379, 4353091

Abstract:

A tissue dissociation device includes an inlet coupled to a first stage having a single channel having an upstream end and a downstream end; a plurality of serially arranged intermediate stages, wherein a first intermediate stage of the plurality is fluidically coupled to the downstream end of the first stage, and wherein each subsequent intermediate stage of the plurality has an increasing number of channels (with channels of smaller dimensions); and an outlet coupled to a last stage of the intermediate stages.