Emily S Gibson

US Patent:

20130149734, Jun 13, 2013

Filed:

May 31, 2011

Appl. No.:

13/700682

Inventors:

David Ammar - Denver CO, US
Malik Kahook - Denver CO, US
Tim Lei - Thornton CO, US
Emily Gibson - Boulder CO, US
Omid Masihzadeh - Lakewood CO, US
Naresh Mandava - Denver CO, US

Assignee:

The Regents of the University of Colorado, a body corporate - Denver CO

International Classification:

A61B 3/00
G01N 21/65
G01N 21/64

US Classification:

435 29, 600476, 2504581, 356 73, 2504591

Abstract:

A multimodal method for imaging tissue comprising: aligning an excitation light source with at least a portion of the tissue; selecting at least two modalities of image acquisition; imaging the tissue portion with each of the modalities of image acquisition; and constructing a dual mode image using images from each of the modalities of image acquisition. A multimodal system for imaging tissue comprising: an excitation light source or light sources; an optical and alignment system for directing the excitation beam or beams to a sample and receiving an emission beam from the sample; at least one detector for receiving the emission beam from the sample; and a spectral filtering or dispersing device for providing at least two imaging modalities at the at least one detector; and a processor for analyzing the detected emission beam and constructing a dual mode image using images from each of the modalities of image acquisition.

US Patent:

20110207207, Aug 25, 2011

Filed:

Oct 28, 2009

Appl. No.:

13/126412

Inventors:

Emily A. Gibson - Boulder CO, US
Tim C. Lei - Thornton CO, US

International Classification:

C12M 1/34

US Classification:

4352883, 4352887

Abstract:

A microfluidic cell sorter has a microfluidic structure () with a sample input channel () leading into an observation region (), two buffer channels () configured to hydrodynamically focus a sample target cell () within the observation region, and at least two output channels (). Apparatus directs the target cell into a selected output channel based on a cell sorting control signal (). A CARS pulse source () generates CARS pulses (), which are directed to the target cell within the observation region. A detector () detects CARS illumination scattered from the target signal and generates a spectrum signal based on the detected illumination. A processor () identifies the target cell based on the spectrum signal and generates the cell sorting control signal based on the identity of the target cell.

US Patent:

20210372927, Dec 2, 2021

Filed:

Mar 1, 2019

Appl. No.:

16/976946

Inventors:

- Denver CO, US
Brendan M. HEFFERNAN - Boulder CO, US
Robert NIEDERRITER - Los Angeles CA, US
Stephanie A. MEYER - Denver CO, US
Diego RESTREPO - Littleton CO, US
Emily A. GIBSON - Boulder CO, US
Mark E. SIEMENS - Denver CO, US

International Classification:

G01N 21/64
G02B 21/00
G02B 27/58
G02B 3/00

Abstract:

Sub-diffraction limited fluorescent images using a fiber-based stimulated emission depletion (STED) microscope are reported. Both excitation and depletion beams are transported through polarization-maintaining fiber and a lateral resolution of 100 nm has been achieved.

US Patent:

20200348505, Nov 5, 2020

Filed:

Apr 20, 2020

Appl. No.:

16/853512

Inventors:

- Denver CO, US
Emily A. Gibson - Boulder CO, US
Victor M. Bright - Boulder CO, US
Richard Weir - Longmont CO, US
Diego Restrepo - Littleton CO, US
Baris Ozbay - Denver CO, US

International Classification:

G02B 23/24
G02B 3/14
G02B 26/00
G02B 6/06
G02B 21/00
G02B 23/26
H04N 5/225

Abstract:

The present disclosure relates optical imaging devices and methods useful in biological and medical imaging applications. In one embodiment, an optical imaging device includes a flexible lightguide having a first end and a second end, the output of the source of pulsed infrared radiation being optically coupled to the first end of the flexible lightguide; a lens assembly attached to and optically coupled to the second end of the flexible lightguide, the lens assembly comprising a variable-focus lens element, the a variable-focus lens element having a tunable focal length; and a photodetector coupled to the flexible lightguide to detect radiation propagating from the second end toward the first end of the flexible lightguide. The optical imaging devices and methods can be used in both confocal and multi-photon techniques.

US Patent:

20200292810, Sep 17, 2020

Filed:

Oct 3, 2018

Appl. No.:

16/753615

Inventors:

- Denver CO, US
Mo Zohrabi - Boulder CO, US
Victor M. Bright - Boulder CO, US
Omkar Supekar - Boulder CO, US
Robert H. Cormack - Erie CO, US
Emily Gibson - Boulder CO, US
Connor McCullough - Denver CO, US

International Classification:

G02B 27/00
G02B 21/06

Abstract:

The present disclosure relates generally to methods and systems useful in imaging applications, especially biological imaging applications, and applications in the metrology, atmospheric, scientific and medical fields. In one aspect, the disclosure provides a method of imaging an object, including illuminating the object with incident radiation through one or more adaptive optical elements; receiving transmitted radiation from the object at a photodetector to provide a base image; and performing the following steps one or more times: adjusting the one or more adaptive optical elements, the adjustment including modifying an optical transfer function of the one or more adaptive optical elements, and receiving transmitted radiation from the object at the photodetector to provide an adjusted image; wherein the adjustment and receiving steps are performed until the adjusted image has substantially reduced aberrations compared to the base image.

US Patent:

20170356827, Dec 14, 2017

Filed:

Nov 19, 2015

Appl. No.:

15/527217

Inventors:

- Denver CO, US
Emily A. GIBSON - Boulder CO, US
Kian BEHBAKHT - Greenwood Village CO, US

International Classification:

G01N 1/31
G06K 9/00
G01N 1/40

Abstract:

Devices and methods for labeling and mounting suspended cells in a controllable area are disclosed. The devices and methods utilize polycarbonate filters. The filters are employed both to capture the cells and as a substrate for labeling. This disclosure provides a device for cell capture and staining. This device utilizes a stack comprising a filter sandwiched between two o-rings (an “OFO stack”) in which the o-rings both seat the device and, based on their outer diameter and cross-section, determine the cell capture area. In one embodiment, an alignment plate is affixed to an output head of the device, the alignment plate having one or more through holes, a diameter of the one or more through holes matching an outer diameter of the OFO stack.

US Patent:

20170010456, Jan 12, 2017

Filed:

Jan 22, 2015

Appl. No.:

15/113303

Inventors:

- Denver CO, US
Emily A. Gibson - Boulder CO, US
Victor M. Bright - Boulder CO, US
Richard Weir - Longmont CO, US
Diego Restrepo - Littleton CO, US

International Classification:

G02B 23/24
G02B 21/00
H04N 5/225
G02B 3/14
G02B 6/06
G02B 23/26
G02B 26/00

Abstract:

The present disclosure relates optical imaging devices and methods useful in biological and medical imaging applications. In one embodiment, an optical imaging device includes a flexible lightguide having a first end and a second end, the output of the source of pulsed infrared radiation being optically coupled to the first end of the flexible lightguide; a lens assembly attached to and optically coupled to the second end of the flexible lightguide, the lens assembly comprising a variable-focus lens element, the a variable-focus lens element having a tunable focal length; and a photodetector coupled to the flexible lightguide to detect radiation propagating from the second end toward the first end of the flexible lightguide. The optical imaging devices and methods can be used in both confocal and multi-photon techniques.