Emily Lin

US Patent:

20130164740, Jun 27, 2013

Filed:

Dec 19, 2012

Appl. No.:

13/720615

Inventors:

Abbott Laboratories - Abbott Park IL, US
Emily H. Lin - Cupertino CA, US
Jihping Yang - Palo Alto CA, US

Assignee:

ABBOTT LABORATORIES - Abbott Park IL

International Classification:

C12Q 1/04

US Classification:

435 61, 435377, 4352872

Abstract:

Herein is provided a simple, reliable and accurate method for cellular analysis on hematology analyzers. In various aspects, the methods provide separation and/or differentiation between red blood cells (RBCs) and white blood cells (WBCs) by utilizing a fluorescent dye to selectively stain WBCs such that they emit stronger fluorescence signals. The method provides optimal detection limits on WBCs and RBCs, thereby allowing analysis of samples with sparse cellular concentrations. As few as one reagent may be used to prepare a single dilution for body fluid analysis, in order to simplify the body fluid analysis. Minimal damage to WBCs is attained using the lysis-free approach described in aspects of the disclosure.

US Patent:

20200141858, May 7, 2020

Filed:

Oct 15, 2019

Appl. No.:

16/653489

Inventors:

- Abbott Park IL, US
Marilou Coleman - Newark CA, US
Emily H. Lin - Cupertino CA, US
Michael R. Buhl - San Ramon CA, US
Giacomo Vacca - San Jose CA, US

International Classification:

G01N 15/14
G01N 33/80
G01N 21/64
G01N 33/49

Abstract:

Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs.

US Patent:

20180128732, May 10, 2018

Filed:

Sep 14, 2017

Appl. No.:

15/704974

Inventors:

- Abbott Park IL, US
Marilou Coleman - Newark CA, US
Emily H. Lin - Cupertino CA, US
Michael R. Buhl - San Ramon CA, US
Giacomo Vacca - San Jose CA, US

International Classification:

G01N 15/14
G01N 21/64
G01N 33/49
G01N 33/80
G01N 15/00
G01N 15/10

Abstract:

Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs.

US Patent:

20160018312, Jan 21, 2016

Filed:

Jul 24, 2015

Appl. No.:

14/808720

Inventors:

- Abbott Park IL, US
Marilou Coleman - Newark CA, US
Emily H. Lin - Cupertino CA, US
Michael R. Buhl - San Ramon CA, US
Giacomo Vacca - San Jose CA, US

International Classification:

G01N 15/14
G01N 21/64
G01N 33/49

Abstract:

Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs.